GeneXpert on patients with human immunodeficiency virus and smear-negative pulmonary tuberculosis.

<h4>Objectives</h4>Vietnam is a high-prevalence country for tuberculosis (TB). Xpert MTB/RIF is a novel PCR-based diagnostic test that is substantially more sensitive for detecting M. tuberculosis than traditional smear-based techniques. However, locally-derived evidence of Xpert MTB/RIF...

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Autores principales:	Nguyen Kim Cuong, Nguyen Bao Ngoc, Nguyen Binh Hoa, Vu Quoc Dat, Nguyen Viet Nhung
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/49d9d47d80454a85aae5d2b314613fca
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spelling	oai:doaj.org-article:49d9d47d80454a85aae5d2b314613fca2021-12-02T20:09:39ZGeneXpert on patients with human immunodeficiency virus and smear-negative pulmonary tuberculosis.1932-620310.1371/journal.pone.0253961https://doaj.org/article/49d9d47d80454a85aae5d2b314613fca2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0253961https://doaj.org/toc/1932-6203<h4>Objectives</h4>Vietnam is a high-prevalence country for tuberculosis (TB). Xpert MTB/RIF is a novel PCR-based diagnostic test that is substantially more sensitive for detecting M. tuberculosis than traditional smear-based techniques. However, locally-derived evidence of Xpert MTB/RIF in HIV-infected people is limited. This study evaluates the performance of the Xpert MTB/RIF in HIV-infected patients with smear-negative pulmonary TB (SNTB).<h4>Methods</h4>This was a cross-sectional study in 3 hospitals. The performance of Xpert MTB/RIF was compared with the reference standard of liquid culture and phenotypic drug-susceptibility testing for rifampicin (RIF) resistance.<h4>Results</h4>Out of 123 patients, the median age was 37.0 (IQR: 32.0-41.0) and 81.3% were male. The area under the receiver operating characteristic curve, sensitivity (Se) and specificity (Sp) of Xpert MTB/RIF for pulmonary TB diagnosis were 0.72 (95% confidence interval [CI]: 0.63-0.81), 66.7% (95%CI: 54.8-77.1) and 77.1% (95%CI: 62.7-88.0), respectively, while Se and Sp of Xpert MTB/RIF in detecting RIF resistance were 50.0 (11.8-88.2) and 86.4% (95%CI: 72.7-94.8).<h4>Conclusion</h4>The performance of Xpert MTB/RIF in HIV-infected patients with SNTB for the diagnosis of TB and RIF-resistance was low. Further studies are required to evaluate the results of Xpert MTB/RIF assay in HIV-infected patients with SNTB and the role of Xpert repetition on the same specimens.Nguyen Kim CuongNguyen Bao NgocNguyen Binh HoaVu Quoc DatNguyen Viet NhungPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0253961 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nguyen Kim Cuong Nguyen Bao Ngoc Nguyen Binh Hoa Vu Quoc Dat Nguyen Viet Nhung GeneXpert on patients with human immunodeficiency virus and smear-negative pulmonary tuberculosis.
description	<h4>Objectives</h4>Vietnam is a high-prevalence country for tuberculosis (TB). Xpert MTB/RIF is a novel PCR-based diagnostic test that is substantially more sensitive for detecting M. tuberculosis than traditional smear-based techniques. However, locally-derived evidence of Xpert MTB/RIF in HIV-infected people is limited. This study evaluates the performance of the Xpert MTB/RIF in HIV-infected patients with smear-negative pulmonary TB (SNTB).<h4>Methods</h4>This was a cross-sectional study in 3 hospitals. The performance of Xpert MTB/RIF was compared with the reference standard of liquid culture and phenotypic drug-susceptibility testing for rifampicin (RIF) resistance.<h4>Results</h4>Out of 123 patients, the median age was 37.0 (IQR: 32.0-41.0) and 81.3% were male. The area under the receiver operating characteristic curve, sensitivity (Se) and specificity (Sp) of Xpert MTB/RIF for pulmonary TB diagnosis were 0.72 (95% confidence interval [CI]: 0.63-0.81), 66.7% (95%CI: 54.8-77.1) and 77.1% (95%CI: 62.7-88.0), respectively, while Se and Sp of Xpert MTB/RIF in detecting RIF resistance were 50.0 (11.8-88.2) and 86.4% (95%CI: 72.7-94.8).<h4>Conclusion</h4>The performance of Xpert MTB/RIF in HIV-infected patients with SNTB for the diagnosis of TB and RIF-resistance was low. Further studies are required to evaluate the results of Xpert MTB/RIF assay in HIV-infected patients with SNTB and the role of Xpert repetition on the same specimens.
format	article
author	Nguyen Kim Cuong Nguyen Bao Ngoc Nguyen Binh Hoa Vu Quoc Dat Nguyen Viet Nhung
author_facet	Nguyen Kim Cuong Nguyen Bao Ngoc Nguyen Binh Hoa Vu Quoc Dat Nguyen Viet Nhung
author_sort	Nguyen Kim Cuong
title	GeneXpert on patients with human immunodeficiency virus and smear-negative pulmonary tuberculosis.
title_short	GeneXpert on patients with human immunodeficiency virus and smear-negative pulmonary tuberculosis.
title_full	GeneXpert on patients with human immunodeficiency virus and smear-negative pulmonary tuberculosis.
title_fullStr	GeneXpert on patients with human immunodeficiency virus and smear-negative pulmonary tuberculosis.
title_full_unstemmed	GeneXpert on patients with human immunodeficiency virus and smear-negative pulmonary tuberculosis.
title_sort	genexpert on patients with human immunodeficiency virus and smear-negative pulmonary tuberculosis.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/49d9d47d80454a85aae5d2b314613fca
work_keys_str_mv	AT nguyenkimcuong genexpertonpatientswithhumanimmunodeficiencyvirusandsmearnegativepulmonarytuberculosis AT nguyenbaongoc genexpertonpatientswithhumanimmunodeficiencyvirusandsmearnegativepulmonarytuberculosis AT nguyenbinhhoa genexpertonpatientswithhumanimmunodeficiencyvirusandsmearnegativepulmonarytuberculosis AT vuquocdat genexpertonpatientswithhumanimmunodeficiencyvirusandsmearnegativepulmonarytuberculosis AT nguyenvietnhung genexpertonpatientswithhumanimmunodeficiencyvirusandsmearnegativepulmonarytuberculosis
_version_	1718375058718588928

GeneXpert on patients with human immunodeficiency virus and smear-negative pulmonary tuberculosis.

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