Increased cis-to-trans urocanic acid ratio in the skin of chronic spontaneous urticaria patients

Abstract Increased filaggrin expression was found to be correlated with severity scores in chronic spontaneous urticaria (CSU); however, the role of filaggrin breakdown products (FBPs) in CSU has not been studied. We collected stratum corneum (SC) specimens from the volar forearms of 10 CSU patients...

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Autores principales:	Duy Le Pham, Kyung-Min Lim, Kyung-Mi Joo, Hae-Sim Park, Donald Y. M. Leung, Young-Min Ye
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/49dc399cd680436ebabcd0c53ac90718
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spelling	oai:doaj.org-article:49dc399cd680436ebabcd0c53ac907182021-12-02T12:32:03ZIncreased cis-to-trans urocanic acid ratio in the skin of chronic spontaneous urticaria patients10.1038/s41598-017-01487-92045-2322https://doaj.org/article/49dc399cd680436ebabcd0c53ac907182017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01487-9https://doaj.org/toc/2045-2322Abstract Increased filaggrin expression was found to be correlated with severity scores in chronic spontaneous urticaria (CSU); however, the role of filaggrin breakdown products (FBPs) in CSU has not been studied. We collected stratum corneum (SC) specimens from the volar forearms of 10 CSU patients, 10 AD patients, and 10 healthy normal controls (NCs) and measured contents of FBPs (pyrrolidone carboxylic acid [PCA] and urocanic acid [UCA]) using UPLC-MS/MS, transepidermal water loss (TEWL) and epidermal pH. Compared to NCs, cis-UCA level was increased in CSU lesions (P < 0.05) and decreased in AD lesions (P < 0.01). The cis-to-trans-UCA ratio in SC specimens from CSU patients was significantly greater than those from AD and NC subjects. AD lesions had lower FBP and PCA contents compared to NC skin (both P < 0.001), and higher TEWL and pH compared to CSU lesions. Moreover, cis-UCA, but not trans-UCA, enhanced the IgE-mediated basophil activation, as well as IgE- and calcium-mediated degranulation of LAD-2 cells, in a dose-dependent manner. These findings suggest that increased cis-to-trans UCA ratio in the epidermis is a distinct feature of CSU, which could enhance mast cell degranulation. Modulation of cis-UCA may be a potential target for skin diseases associated with IgE-mediated mast cell degranulation.Duy Le PhamKyung-Min LimKyung-Mi JooHae-Sim ParkDonald Y. M. LeungYoung-Min YeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Duy Le Pham Kyung-Min Lim Kyung-Mi Joo Hae-Sim Park Donald Y. M. Leung Young-Min Ye Increased cis-to-trans urocanic acid ratio in the skin of chronic spontaneous urticaria patients
description	Abstract Increased filaggrin expression was found to be correlated with severity scores in chronic spontaneous urticaria (CSU); however, the role of filaggrin breakdown products (FBPs) in CSU has not been studied. We collected stratum corneum (SC) specimens from the volar forearms of 10 CSU patients, 10 AD patients, and 10 healthy normal controls (NCs) and measured contents of FBPs (pyrrolidone carboxylic acid [PCA] and urocanic acid [UCA]) using UPLC-MS/MS, transepidermal water loss (TEWL) and epidermal pH. Compared to NCs, cis-UCA level was increased in CSU lesions (P < 0.05) and decreased in AD lesions (P < 0.01). The cis-to-trans-UCA ratio in SC specimens from CSU patients was significantly greater than those from AD and NC subjects. AD lesions had lower FBP and PCA contents compared to NC skin (both P < 0.001), and higher TEWL and pH compared to CSU lesions. Moreover, cis-UCA, but not trans-UCA, enhanced the IgE-mediated basophil activation, as well as IgE- and calcium-mediated degranulation of LAD-2 cells, in a dose-dependent manner. These findings suggest that increased cis-to-trans UCA ratio in the epidermis is a distinct feature of CSU, which could enhance mast cell degranulation. Modulation of cis-UCA may be a potential target for skin diseases associated with IgE-mediated mast cell degranulation.
format	article
author	Duy Le Pham Kyung-Min Lim Kyung-Mi Joo Hae-Sim Park Donald Y. M. Leung Young-Min Ye
author_facet	Duy Le Pham Kyung-Min Lim Kyung-Mi Joo Hae-Sim Park Donald Y. M. Leung Young-Min Ye
author_sort	Duy Le Pham
title	Increased cis-to-trans urocanic acid ratio in the skin of chronic spontaneous urticaria patients
title_short	Increased cis-to-trans urocanic acid ratio in the skin of chronic spontaneous urticaria patients
title_full	Increased cis-to-trans urocanic acid ratio in the skin of chronic spontaneous urticaria patients
title_fullStr	Increased cis-to-trans urocanic acid ratio in the skin of chronic spontaneous urticaria patients
title_full_unstemmed	Increased cis-to-trans urocanic acid ratio in the skin of chronic spontaneous urticaria patients
title_sort	increased cis-to-trans urocanic acid ratio in the skin of chronic spontaneous urticaria patients
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/49dc399cd680436ebabcd0c53ac90718
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Increased cis-to-trans urocanic acid ratio in the skin of chronic spontaneous urticaria patients

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