Estimating dose-specific cell division and apoptosis rates from chemo-sensitivity experiments

Abstract In-vitro chemo-sensitivity experiments are an essential step in the early stages of cancer therapy development, but existing data analysis methods suffer from problems with fitting, do not permit assessment of uncertainty, and can give misleading estimates of cell growth inhibition. We pres...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yiyi Liu, Forrest W. Crawford
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/49deb11bf7ae455090e308a5693ea20e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:49deb11bf7ae455090e308a5693ea20e
record_format	dspace
spelling	oai:doaj.org-article:49deb11bf7ae455090e308a5693ea20e2021-12-02T15:08:53ZEstimating dose-specific cell division and apoptosis rates from chemo-sensitivity experiments10.1038/s41598-018-21017-52045-2322https://doaj.org/article/49deb11bf7ae455090e308a5693ea20e2018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-21017-5https://doaj.org/toc/2045-2322Abstract In-vitro chemo-sensitivity experiments are an essential step in the early stages of cancer therapy development, but existing data analysis methods suffer from problems with fitting, do not permit assessment of uncertainty, and can give misleading estimates of cell growth inhibition. We present an approach (bdChemo) based on a mechanistic model of cell division and death that permits rigorous statistical analyses of chemo-sensitivity experiment data by simultaneous estimation of cell division and apoptosis rates as functions of dose, without making strong assumptions about the shape of the dose-response curve. We demonstrate the utility of this method using a large-scale NCI-DREAM challenge dataset. We developed an R package “bdChemo” implementing this method, available at https://github.com/YiyiLiu1/bdChemo.Yiyi LiuForrest W. CrawfordNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yiyi Liu Forrest W. Crawford Estimating dose-specific cell division and apoptosis rates from chemo-sensitivity experiments
description	Abstract In-vitro chemo-sensitivity experiments are an essential step in the early stages of cancer therapy development, but existing data analysis methods suffer from problems with fitting, do not permit assessment of uncertainty, and can give misleading estimates of cell growth inhibition. We present an approach (bdChemo) based on a mechanistic model of cell division and death that permits rigorous statistical analyses of chemo-sensitivity experiment data by simultaneous estimation of cell division and apoptosis rates as functions of dose, without making strong assumptions about the shape of the dose-response curve. We demonstrate the utility of this method using a large-scale NCI-DREAM challenge dataset. We developed an R package “bdChemo” implementing this method, available at https://github.com/YiyiLiu1/bdChemo.
format	article
author	Yiyi Liu Forrest W. Crawford
author_facet	Yiyi Liu Forrest W. Crawford
author_sort	Yiyi Liu
title	Estimating dose-specific cell division and apoptosis rates from chemo-sensitivity experiments
title_short	Estimating dose-specific cell division and apoptosis rates from chemo-sensitivity experiments
title_full	Estimating dose-specific cell division and apoptosis rates from chemo-sensitivity experiments
title_fullStr	Estimating dose-specific cell division and apoptosis rates from chemo-sensitivity experiments
title_full_unstemmed	Estimating dose-specific cell division and apoptosis rates from chemo-sensitivity experiments
title_sort	estimating dose-specific cell division and apoptosis rates from chemo-sensitivity experiments
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/49deb11bf7ae455090e308a5693ea20e
work_keys_str_mv	AT yiyiliu estimatingdosespecificcelldivisionandapoptosisratesfromchemosensitivityexperiments AT forrestwcrawford estimatingdosespecificcelldivisionandapoptosisratesfromchemosensitivityexperiments
_version_	1718387995586854912

Estimating dose-specific cell division and apoptosis rates from chemo-sensitivity experiments

Ejemplares similares