Prolonged oral ingestion of microplastics induced inflammation in the liver tissues of C57BL/6J mice through polarization of macrophages and increased infiltration of natural killer cells

Microplastics (< 5 mm diameter) are one of most important environmental pollutants and contaminants worldwide. However, how microplastics affect liver immune microenvironment in not well understood. Microplastics (0.5 µm) were administered orally to C57BL/6J mice for 4 consecutive weeks at the ra...

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Autores principales:	Liangtao Zhao, Wenyuan Shi, Fangfang Hu, Xujun Song, Zhangjun Cheng, Jiahua Zhou
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Microplastics Macrophages Natural killer Inflammation Liver Environmental pollution TD172-193.5 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/49ea15201a694cb6966fa4d168902318
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spelling	oai:doaj.org-article:49ea15201a694cb6966fa4d1689023182021-11-06T04:14:30ZProlonged oral ingestion of microplastics induced inflammation in the liver tissues of C57BL/6J mice through polarization of macrophages and increased infiltration of natural killer cells0147-651310.1016/j.ecoenv.2021.112882https://doaj.org/article/49ea15201a694cb6966fa4d1689023182021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0147651321009945https://doaj.org/toc/0147-6513Microplastics (< 5 mm diameter) are one of most important environmental pollutants and contaminants worldwide. However, how microplastics affect liver immune microenvironment in not well understood. Microplastics (0.5 µm) were administered orally to C57BL/6J mice for 4 consecutive weeks at the rate of 0.5 mg/day. Non-parenchymal cells were isolated from of the mice through fractionation of fresh hepatic tissues. The immune landscape for four cell populations of B cells, T cells, NK cells and macrophages in the liver tissues was then evaluated using flow cytometry. The secretion level of inflammatory cytokines and associated signaling pathway were investigated using quantitative real-time polymerase chain reaction and western blot. Oral ingestion of microplastics increases liver weight, general liver index as well as expression of serum, liver function-related indicators. Microplastics also increased the infiltration of natural killer cells and macrophages to non-parenchymal liver cells, but reduced that of B cells to the same tissues. However, microplastics had no effect on the infiltration of T cell to non-parenchymal liver cells. Ingestion of MPs also up-regulated the expression of IFN-γ, TNF-α, IL-1β, IL-6 and IL-33 mRNA, but down-regulated that of IL-4, IL-5, IL-10, IL-18 and TGF-β1. Overall, the aforementioned processes were regulated via the NF-κB pathway in the hepatic non-parenchymal cells. Microplastics disrupts inflammatory process in liver tissues via the NF-κB signaling pathway. These findings provide a strong foundation on immune processes in hepatic tissues following prolonged ingestion of microplastics.Liangtao ZhaoWenyuan ShiFangfang HuXujun SongZhangjun ChengJiahua ZhouElsevierarticleMicroplasticsMacrophagesNatural killerInflammationLiverEnvironmental pollutionTD172-193.5Environmental sciencesGE1-350ENEcotoxicology and Environmental Safety, Vol 227, Iss , Pp 112882- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microplastics Macrophages Natural killer Inflammation Liver Environmental pollution TD172-193.5 Environmental sciences GE1-350
spellingShingle	Microplastics Macrophages Natural killer Inflammation Liver Environmental pollution TD172-193.5 Environmental sciences GE1-350 Liangtao Zhao Wenyuan Shi Fangfang Hu Xujun Song Zhangjun Cheng Jiahua Zhou Prolonged oral ingestion of microplastics induced inflammation in the liver tissues of C57BL/6J mice through polarization of macrophages and increased infiltration of natural killer cells
description	Microplastics (< 5 mm diameter) are one of most important environmental pollutants and contaminants worldwide. However, how microplastics affect liver immune microenvironment in not well understood. Microplastics (0.5 µm) were administered orally to C57BL/6J mice for 4 consecutive weeks at the rate of 0.5 mg/day. Non-parenchymal cells were isolated from of the mice through fractionation of fresh hepatic tissues. The immune landscape for four cell populations of B cells, T cells, NK cells and macrophages in the liver tissues was then evaluated using flow cytometry. The secretion level of inflammatory cytokines and associated signaling pathway were investigated using quantitative real-time polymerase chain reaction and western blot. Oral ingestion of microplastics increases liver weight, general liver index as well as expression of serum, liver function-related indicators. Microplastics also increased the infiltration of natural killer cells and macrophages to non-parenchymal liver cells, but reduced that of B cells to the same tissues. However, microplastics had no effect on the infiltration of T cell to non-parenchymal liver cells. Ingestion of MPs also up-regulated the expression of IFN-γ, TNF-α, IL-1β, IL-6 and IL-33 mRNA, but down-regulated that of IL-4, IL-5, IL-10, IL-18 and TGF-β1. Overall, the aforementioned processes were regulated via the NF-κB pathway in the hepatic non-parenchymal cells. Microplastics disrupts inflammatory process in liver tissues via the NF-κB signaling pathway. These findings provide a strong foundation on immune processes in hepatic tissues following prolonged ingestion of microplastics.
format	article
author	Liangtao Zhao Wenyuan Shi Fangfang Hu Xujun Song Zhangjun Cheng Jiahua Zhou
author_facet	Liangtao Zhao Wenyuan Shi Fangfang Hu Xujun Song Zhangjun Cheng Jiahua Zhou
author_sort	Liangtao Zhao
title	Prolonged oral ingestion of microplastics induced inflammation in the liver tissues of C57BL/6J mice through polarization of macrophages and increased infiltration of natural killer cells
title_short	Prolonged oral ingestion of microplastics induced inflammation in the liver tissues of C57BL/6J mice through polarization of macrophages and increased infiltration of natural killer cells
title_full	Prolonged oral ingestion of microplastics induced inflammation in the liver tissues of C57BL/6J mice through polarization of macrophages and increased infiltration of natural killer cells
title_fullStr	Prolonged oral ingestion of microplastics induced inflammation in the liver tissues of C57BL/6J mice through polarization of macrophages and increased infiltration of natural killer cells
title_full_unstemmed	Prolonged oral ingestion of microplastics induced inflammation in the liver tissues of C57BL/6J mice through polarization of macrophages and increased infiltration of natural killer cells
title_sort	prolonged oral ingestion of microplastics induced inflammation in the liver tissues of c57bl/6j mice through polarization of macrophages and increased infiltration of natural killer cells
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/49ea15201a694cb6966fa4d168902318
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Prolonged oral ingestion of microplastics induced inflammation in the liver tissues of C57BL/6J mice through polarization of macrophages and increased infiltration of natural killer cells

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