Exercise-Induced Plasma Metabolomic Profiles in Patients With Peripheral Arterial Disease
Aim: A better knowledge of the biological consequences in the blood of these exercise-induced ischemic events in lower extremity artery disease (LEAD) may improve the prospects of disease management. We explored the preminus postexercise metabolomic difference in 39 patients with LEAD referred for a...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:49ec3dc9791a47a9aeae4c239495f8be2021-11-18T08:35:57ZExercise-Induced Plasma Metabolomic Profiles in Patients With Peripheral Arterial Disease1664-042X10.3389/fphys.2021.758085https://doaj.org/article/49ec3dc9791a47a9aeae4c239495f8be2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphys.2021.758085/fullhttps://doaj.org/toc/1664-042XAim: A better knowledge of the biological consequences in the blood of these exercise-induced ischemic events in lower extremity artery disease (LEAD) may improve the prospects of disease management. We explored the preminus postexercise metabolomic difference in 39 patients with LEAD referred for a treadmill oximetry test [transcutaneous oximetry (TcPO2)].Methods: Ischemia was estimated through the sum of decrease from rest of oxygen pressure (DROPs) (limb TcPO2 changes minus chest TcPO2 changes) at buttocks, thighs, and calves regions. Targeted metabolomic analyses measuring 188 metabolites were performed on a few microliters blood samples taken at the earlobe at rest and 3 min after exercise.Results: Maximum walking distance (MWD) was 290 m (120–652 m) and ankle brachial index (ABI) was 0.67 ± 0.17. Supervised paired partial least squares discriminant analysis based on 23,345 models showed good predictive performance for test sets with a median area under the receiver operating characteristic (AUROC) curve value of 0.99 and a p-value of 0.00049. The best discriminant metabolites contributing to the model included a subset of 71 (47%) of the 150 accurately measured metabolites in the plasma, comprising 3 acylcarnitines, 3 amino acids, 5 biogenic amines, 9 sphingomyelin, 7 lysophosphatidylcholines, and 44 phosphatidylcholines. In addition, 16 of these metabolites were found to correlate with one or more severity scores of the LEAD.Conclusion: Our results provide new insights into the biological changes that accompany exercise in LEAD and contribute to a better understanding of walking impairment pathophysiology in LEAD, highlighting new candidate biomarkers.Wendsèndaté Yves SemporéWendsèndaté Yves SemporéWendsèndaté Yves SemporéJuan Manuel Chao De La BarcaJuan Manuel Chao De La BarcaJeanne HersantNafi OuédraogoTéné Marceline YaméogoSamir HenniSamir HenniPascal ReynierPascal ReynierPierre AbrahamPierre AbrahamPierre AbrahamFrontiers Media S.A.articlewalking impairmentexercisetreadmill testtranscutaneous oximetrymetabolomic profilePhysiologyQP1-981ENFrontiers in Physiology, Vol 12 (2021) |
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walking impairment exercise treadmill test transcutaneous oximetry metabolomic profile Physiology QP1-981 |
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walking impairment exercise treadmill test transcutaneous oximetry metabolomic profile Physiology QP1-981 Wendsèndaté Yves Semporé Wendsèndaté Yves Semporé Wendsèndaté Yves Semporé Juan Manuel Chao De La Barca Juan Manuel Chao De La Barca Jeanne Hersant Nafi Ouédraogo Téné Marceline Yaméogo Samir Henni Samir Henni Pascal Reynier Pascal Reynier Pierre Abraham Pierre Abraham Pierre Abraham Exercise-Induced Plasma Metabolomic Profiles in Patients With Peripheral Arterial Disease |
| description |
Aim: A better knowledge of the biological consequences in the blood of these exercise-induced ischemic events in lower extremity artery disease (LEAD) may improve the prospects of disease management. We explored the preminus postexercise metabolomic difference in 39 patients with LEAD referred for a treadmill oximetry test [transcutaneous oximetry (TcPO2)].Methods: Ischemia was estimated through the sum of decrease from rest of oxygen pressure (DROPs) (limb TcPO2 changes minus chest TcPO2 changes) at buttocks, thighs, and calves regions. Targeted metabolomic analyses measuring 188 metabolites were performed on a few microliters blood samples taken at the earlobe at rest and 3 min after exercise.Results: Maximum walking distance (MWD) was 290 m (120–652 m) and ankle brachial index (ABI) was 0.67 ± 0.17. Supervised paired partial least squares discriminant analysis based on 23,345 models showed good predictive performance for test sets with a median area under the receiver operating characteristic (AUROC) curve value of 0.99 and a p-value of 0.00049. The best discriminant metabolites contributing to the model included a subset of 71 (47%) of the 150 accurately measured metabolites in the plasma, comprising 3 acylcarnitines, 3 amino acids, 5 biogenic amines, 9 sphingomyelin, 7 lysophosphatidylcholines, and 44 phosphatidylcholines. In addition, 16 of these metabolites were found to correlate with one or more severity scores of the LEAD.Conclusion: Our results provide new insights into the biological changes that accompany exercise in LEAD and contribute to a better understanding of walking impairment pathophysiology in LEAD, highlighting new candidate biomarkers. |
| format |
article |
| author |
Wendsèndaté Yves Semporé Wendsèndaté Yves Semporé Wendsèndaté Yves Semporé Juan Manuel Chao De La Barca Juan Manuel Chao De La Barca Jeanne Hersant Nafi Ouédraogo Téné Marceline Yaméogo Samir Henni Samir Henni Pascal Reynier Pascal Reynier Pierre Abraham Pierre Abraham Pierre Abraham |
| author_facet |
Wendsèndaté Yves Semporé Wendsèndaté Yves Semporé Wendsèndaté Yves Semporé Juan Manuel Chao De La Barca Juan Manuel Chao De La Barca Jeanne Hersant Nafi Ouédraogo Téné Marceline Yaméogo Samir Henni Samir Henni Pascal Reynier Pascal Reynier Pierre Abraham Pierre Abraham Pierre Abraham |
| author_sort |
Wendsèndaté Yves Semporé |
| title |
Exercise-Induced Plasma Metabolomic Profiles in Patients With Peripheral Arterial Disease |
| title_short |
Exercise-Induced Plasma Metabolomic Profiles in Patients With Peripheral Arterial Disease |
| title_full |
Exercise-Induced Plasma Metabolomic Profiles in Patients With Peripheral Arterial Disease |
| title_fullStr |
Exercise-Induced Plasma Metabolomic Profiles in Patients With Peripheral Arterial Disease |
| title_full_unstemmed |
Exercise-Induced Plasma Metabolomic Profiles in Patients With Peripheral Arterial Disease |
| title_sort |
exercise-induced plasma metabolomic profiles in patients with peripheral arterial disease |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/49ec3dc9791a47a9aeae4c239495f8be |
| work_keys_str_mv |
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