New insight on biological interaction analysis: new nanocrystalline mixed metal oxide SPME fiber for GC-FID analysis of BTEX and its application in human hemoglobin-benzene interaction studies.

Nanocrystalline mixed metal oxides (MMO) of various metal cations were synthesized and were used for coating a piece of copper wire as a new high sensitive solid phase micro extraction (SPME) fiber in extraction and determination of BTEX compounds from the headspace of aqueous samples prior to GC-FI...

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Autores principales:	Reza Hosseinzadeh, Ali Akbar Moosavi Movahedi, Hedayatollah Ghourchian
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/49ee74950b074f02b34e7da2c831c0ad
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spelling	oai:doaj.org-article:49ee74950b074f02b34e7da2c831c0ad2021-11-25T06:07:01ZNew insight on biological interaction analysis: new nanocrystalline mixed metal oxide SPME fiber for GC-FID analysis of BTEX and its application in human hemoglobin-benzene interaction studies.1932-620310.1371/journal.pone.0102992https://doaj.org/article/49ee74950b074f02b34e7da2c831c0ad2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25068260/?tool=EBIhttps://doaj.org/toc/1932-6203Nanocrystalline mixed metal oxides (MMO) of various metal cations were synthesized and were used for coating a piece of copper wire as a new high sensitive solid phase micro extraction (SPME) fiber in extraction and determination of BTEX compounds from the headspace of aqueous samples prior to GC-FID analysis. Under optimum extraction conditions, the proposed fiber exhibited low detection limits, and quantification limits, good reproducibility, simple and fast preparation method, high fiber capacity and high thermal and mechanical durability. These are some of the most important advantages of the new fiber. The proposed fiber was used for human hemoglobin upon interaction with benzene. Binding isotherm, Scatchard and Klotz logarithmic plots were constructed using HS-SPME-GC data, accurately. The obtained binding isotherm analyzed using Hill method. The Hill parameters have been obtained by calculating saturation parameter from the ratio of measured chromatographic peak areas in the presence and absence of hemoglobin. In this interaction, Hill coefficient and Hill constant determined as (nH = 6.14 and log KH = 6.47) respectively. These results reveal the cooperativity of hemoglobin upon interaction with benzene.Reza HosseinzadehAli Akbar Moosavi MovahediHedayatollah GhourchianPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e102992 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Reza Hosseinzadeh Ali Akbar Moosavi Movahedi Hedayatollah Ghourchian New insight on biological interaction analysis: new nanocrystalline mixed metal oxide SPME fiber for GC-FID analysis of BTEX and its application in human hemoglobin-benzene interaction studies.
description	Nanocrystalline mixed metal oxides (MMO) of various metal cations were synthesized and were used for coating a piece of copper wire as a new high sensitive solid phase micro extraction (SPME) fiber in extraction and determination of BTEX compounds from the headspace of aqueous samples prior to GC-FID analysis. Under optimum extraction conditions, the proposed fiber exhibited low detection limits, and quantification limits, good reproducibility, simple and fast preparation method, high fiber capacity and high thermal and mechanical durability. These are some of the most important advantages of the new fiber. The proposed fiber was used for human hemoglobin upon interaction with benzene. Binding isotherm, Scatchard and Klotz logarithmic plots were constructed using HS-SPME-GC data, accurately. The obtained binding isotherm analyzed using Hill method. The Hill parameters have been obtained by calculating saturation parameter from the ratio of measured chromatographic peak areas in the presence and absence of hemoglobin. In this interaction, Hill coefficient and Hill constant determined as (nH = 6.14 and log KH = 6.47) respectively. These results reveal the cooperativity of hemoglobin upon interaction with benzene.
format	article
author	Reza Hosseinzadeh Ali Akbar Moosavi Movahedi Hedayatollah Ghourchian
author_facet	Reza Hosseinzadeh Ali Akbar Moosavi Movahedi Hedayatollah Ghourchian
author_sort	Reza Hosseinzadeh
title	New insight on biological interaction analysis: new nanocrystalline mixed metal oxide SPME fiber for GC-FID analysis of BTEX and its application in human hemoglobin-benzene interaction studies.
title_short	New insight on biological interaction analysis: new nanocrystalline mixed metal oxide SPME fiber for GC-FID analysis of BTEX and its application in human hemoglobin-benzene interaction studies.
title_full	New insight on biological interaction analysis: new nanocrystalline mixed metal oxide SPME fiber for GC-FID analysis of BTEX and its application in human hemoglobin-benzene interaction studies.
title_fullStr	New insight on biological interaction analysis: new nanocrystalline mixed metal oxide SPME fiber for GC-FID analysis of BTEX and its application in human hemoglobin-benzene interaction studies.
title_full_unstemmed	New insight on biological interaction analysis: new nanocrystalline mixed metal oxide SPME fiber for GC-FID analysis of BTEX and its application in human hemoglobin-benzene interaction studies.
title_sort	new insight on biological interaction analysis: new nanocrystalline mixed metal oxide spme fiber for gc-fid analysis of btex and its application in human hemoglobin-benzene interaction studies.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/49ee74950b074f02b34e7da2c831c0ad
work_keys_str_mv	AT rezahosseinzadeh newinsightonbiologicalinteractionanalysisnewnanocrystallinemixedmetaloxidespmefiberforgcfidanalysisofbtexanditsapplicationinhumanhemoglobinbenzeneinteractionstudies AT aliakbarmoosavimovahedi newinsightonbiologicalinteractionanalysisnewnanocrystallinemixedmetaloxidespmefiberforgcfidanalysisofbtexanditsapplicationinhumanhemoglobinbenzeneinteractionstudies AT hedayatollahghourchian newinsightonbiologicalinteractionanalysisnewnanocrystallinemixedmetaloxidespmefiberforgcfidanalysisofbtexanditsapplicationinhumanhemoglobinbenzeneinteractionstudies
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New insight on biological interaction analysis: new nanocrystalline mixed metal oxide SPME fiber for GC-FID analysis of BTEX and its application in human hemoglobin-benzene interaction studies.

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