Identification of ISSR-based Molecular Markers Associated with Ploidy Level of Orange Watermelon (<i>Citrullus lanatus</i> (Thunb.) Matsum & Nakai)

Indonesia is an agrarian country whose territory is partly used as an agricultural sector, has especially watermelon (Citrullus lanatus (Thunb.) Matsum & Nakai). At present, the development of watermelons in Indonesia still requires a variety of colors, superior watermelons need to be developed...

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Autores principales:	Andra Jausa Salsabila, Budi Setiadi Daryono, Aprilia Sufi Subiastuti
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Gadjah Mada 2021
Materias:	issr, marker assisted breeding, orange watermelon, ploidy, triploid Agriculture (General) S1-972 Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/49eef2de70aa40deac039b86971f5bb8
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spelling	oai:doaj.org-article:49eef2de70aa40deac039b86971f5bb82021-11-08T08:40:57ZIdentification of ISSR-based Molecular Markers Associated with Ploidy Level of Orange Watermelon (<i>Citrullus lanatus</i> (Thunb.) Matsum & Nakai)2540-95732540-958110.22146/jtbb.61858https://doaj.org/article/49eef2de70aa40deac039b86971f5bb82021-11-01T00:00:00Zhttps://jurnal.ugm.ac.id/jtbb/article/view/61858https://doaj.org/toc/2540-9573https://doaj.org/toc/2540-9581Indonesia is an agrarian country whose territory is partly used as an agricultural sector, has especially watermelon (Citrullus lanatus (Thunb.) Matsum & Nakai). At present, the development of watermelons in Indonesia still requires a variety of colors, superior watermelons need to be developed by making triploid orange watermelons. The new watermelon cultivar (F₁) is the result of crossing 'Jelita' (♀) and 'BallyBall' (♂) which are expected to provide a combination of characters that can produce large and sweet fruits. The purpose of this study was to identify ISSR markers that were associated with ploidy levels of horticultural crops, particularly watermelon. The watermelons were growns on the agricultural land of Kebondalem Hamlet, Madurejo Village, Prambanan District, D.I.Y. and analyzed at the Laboratory of Biotechnology PAU, UGM and Laboratory of Genetics and Breeding, Faculty of Biology UGM. Analysis of genetic variation was carried out using the PCR-ISSR method and the DNA bandwidth was calculated using Paint Apps, Microsoft Excel 2013, and Microsoft Word 2013. The results of crossing 'Jelita' and 'King Quality 'is sterile. The result of 'Jelita' with 'BallyBall' produces tillers until sterile harvest time. The results of the analysis of genetic variation using the PCR-ISSR method indicate that the ISSR BI, B3, B5, and CBTC 1 molecular markers cannot be used to distinguish ploidy from the tested watermelons.Andra Jausa SalsabilaBudi Setiadi DaryonoAprilia Sufi SubiastutiUniversitas Gadjah Madaarticleissr, marker assisted breeding, orange watermelon, ploidy, triploidAgriculture (General)S1-972Plant cultureSB1-1110ENJournal of Tropical Biodiversity and Biotechnology, Vol 6, Iss 3 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	issr, marker assisted breeding, orange watermelon, ploidy, triploid Agriculture (General) S1-972 Plant culture SB1-1110
spellingShingle	issr, marker assisted breeding, orange watermelon, ploidy, triploid Agriculture (General) S1-972 Plant culture SB1-1110 Andra Jausa Salsabila Budi Setiadi Daryono Aprilia Sufi Subiastuti Identification of ISSR-based Molecular Markers Associated with Ploidy Level of Orange Watermelon (<i>Citrullus lanatus</i> (Thunb.) Matsum & Nakai)
description	Indonesia is an agrarian country whose territory is partly used as an agricultural sector, has especially watermelon (Citrullus lanatus (Thunb.) Matsum & Nakai). At present, the development of watermelons in Indonesia still requires a variety of colors, superior watermelons need to be developed by making triploid orange watermelons. The new watermelon cultivar (F₁) is the result of crossing 'Jelita' (♀) and 'BallyBall' (♂) which are expected to provide a combination of characters that can produce large and sweet fruits. The purpose of this study was to identify ISSR markers that were associated with ploidy levels of horticultural crops, particularly watermelon. The watermelons were growns on the agricultural land of Kebondalem Hamlet, Madurejo Village, Prambanan District, D.I.Y. and analyzed at the Laboratory of Biotechnology PAU, UGM and Laboratory of Genetics and Breeding, Faculty of Biology UGM. Analysis of genetic variation was carried out using the PCR-ISSR method and the DNA bandwidth was calculated using Paint Apps, Microsoft Excel 2013, and Microsoft Word 2013. The results of crossing 'Jelita' and 'King Quality 'is sterile. The result of 'Jelita' with 'BallyBall' produces tillers until sterile harvest time. The results of the analysis of genetic variation using the PCR-ISSR method indicate that the ISSR BI, B3, B5, and CBTC 1 molecular markers cannot be used to distinguish ploidy from the tested watermelons.
format	article
author	Andra Jausa Salsabila Budi Setiadi Daryono Aprilia Sufi Subiastuti
author_facet	Andra Jausa Salsabila Budi Setiadi Daryono Aprilia Sufi Subiastuti
author_sort	Andra Jausa Salsabila
title	Identification of ISSR-based Molecular Markers Associated with Ploidy Level of Orange Watermelon (<i>Citrullus lanatus</i> (Thunb.) Matsum & Nakai)
title_short	Identification of ISSR-based Molecular Markers Associated with Ploidy Level of Orange Watermelon (<i>Citrullus lanatus</i> (Thunb.) Matsum & Nakai)
title_full	Identification of ISSR-based Molecular Markers Associated with Ploidy Level of Orange Watermelon (<i>Citrullus lanatus</i> (Thunb.) Matsum & Nakai)
title_fullStr	Identification of ISSR-based Molecular Markers Associated with Ploidy Level of Orange Watermelon (<i>Citrullus lanatus</i> (Thunb.) Matsum & Nakai)
title_full_unstemmed	Identification of ISSR-based Molecular Markers Associated with Ploidy Level of Orange Watermelon (<i>Citrullus lanatus</i> (Thunb.) Matsum & Nakai)
title_sort	identification of issr-based molecular markers associated with ploidy level of orange watermelon (<i>citrullus lanatus</i> (thunb.) matsum & nakai)
publisher	Universitas Gadjah Mada
publishDate	2021
url	https://doaj.org/article/49eef2de70aa40deac039b86971f5bb8
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Identification of ISSR-based Molecular Markers Associated with Ploidy Level of Orange Watermelon (<i>Citrullus lanatus</i> (Thunb.) Matsum & Nakai)

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