Peningkatan dan aktivitas enzim asetilkolinesterase pada nyamuk Aedes aegypti yang diseleksi dengan malation
<p><strong>The Elevated and Activity of Acetilcholinesterase Enzyme on <em>Aedes aegypti</em> Selected by Malathion.</strong> The aim of this research was to study the effect of selection by malathion on the activity level Acetilcholinesterase enzyme on <em>Aedes...
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| Autores principales: | , |
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| Formato: | article |
| Lenguaje: | EN ID |
| Publicado: |
The Entomological Society of Indonesia
2017
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/49f6ea885421497e80aee3d9ead4fd7b |
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| Sumario: | <p><strong>The Elevated and Activity of Acetilcholinesterase Enzyme on <em>Aedes aegypti</em> Selected by Malathion.</strong> The aim of this research was to study the effect of selection by malathion on the activity level Acetilcholinesterase enzyme on <em>Aedes aegypti</em> mosquitoes. Selection of <em>Aedes aegypti</em> larval by mean of malathion have been conducted for 20 generations. During the selection process time has been increased of concentration applied and exposure. For generation 0-5 (F0-F5), a concentration of 25 µl/l (24 ppm) was used to expose the larvae to malathion for five minutes. In generation 6-10 (F6-F10) the concentration has increased to 50 µl/l (48 ppm); in F11-F15 the concentration used was 100 µl/l (96 ppm) whereas in F16-F20 200µl/l (192 ppm) was used. Mosquito generations that would be regarded as representative and reference groups were F0, F5, F10, F15 and F20. The LC<sub>50</sub> of F0, F5, F10, F15 and F20 was 0,025; 0,032; 0,042; 0,062 and 0,071 ppm respectively. Increases LT<sub>50</sub> values was also observed in <em>Aedes aegypti</em> selected by malathion. The LT<sub>50</sub> of F0, F5, F10, F15 and F20 generations was 7,9; 11,3; 18; 30,6 and 33,1 minutes respectively. The low levels of malathion resistance could be conferred by the elevated of α-esterase. The values of the α-esterase in F0, F5, F10, F15 and F20 were 0,155; 0,174; 0,203; 0,209 and 0,215 µmol/min/mg protein respectively. The acetilcholinesterase activities were also raised in F0, F5, F10, F15 and F20, the value of acetilcholinesterase activities were 20,35; 20,26; 23,14; 23,18 and 24,9%.</p> |
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