Peningkatan dan aktivitas enzim asetilkolinesterase pada nyamuk Aedes aegypti yang diseleksi dengan malation

The Elevated and Activity of Acetilcholinesterase Enzyme on Aedes aegypti Selected by Malathion. The aim of this research was to study the effect of selection by malathion on the activity level Acetilcholinesterase enzyme on Aedes...

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Autores principales:	Dwi Jayanti Gunandini, P. B. Wicaksana
Formato:	article
Lenguaje:	EN ID
Publicado:	The Entomological Society of Indonesia 2017
Materias:	Aedes aegypti, malathion, esterase. Zoology QL1-991
Acceso en línea:	https://doaj.org/article/49f6ea885421497e80aee3d9ead4fd7b
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spelling	oai:doaj.org-article:49f6ea885421497e80aee3d9ead4fd7b2021-12-02T02:18:59ZPeningkatan dan aktivitas enzim asetilkolinesterase pada nyamuk Aedes aegypti yang diseleksi dengan malation1829-77222089-025710.5994/jei.2.2.24https://doaj.org/article/49f6ea885421497e80aee3d9ead4fd7b2017-02-01T00:00:00Zhttp://jurnal.pei-pusat.org/index.php/jei/article/view/294https://doaj.org/toc/1829-7722https://doaj.org/toc/2089-0257<p><strong>The Elevated and Activity of Acetilcholinesterase Enzyme on <em>Aedes aegypti</em> Selected by Malathion.</strong> The aim of this research was to study the effect of selection by malathion on the activity level Acetilcholinesterase enzyme on <em>Aedes aegypti</em> mosquitoes. Selection of <em>Aedes aegypti</em> larval by mean of malathion have been conducted for 20 generations. During the selection process time has been increased of concentration applied and exposure. For generation 0-5 (F0-F5), a concentration of 25 µl/l (24 ppm) was used to expose the larvae to malathion for five minutes. In generation 6-10 (F6-F10) the concentration has increased to 50 µl/l (48 ppm); in F11-F15 the concentration used was 100 µl/l (96 ppm) whereas in F16-F20 200µl/l (192 ppm) was used. Mosquito generations that would be regarded as representative and reference groups were F0, F5, F10, F15 and F20. The LC<sub>50</sub> of F0, F5, F10, F15 and F20 was 0,025; 0,032; 0,042; 0,062 and 0,071 ppm respectively. Increases LT<sub>50</sub> values was also observed in <em>Aedes aegypti</em> selected by malathion. The LT<sub>50</sub> of F0, F5, F10, F15 and F20 generations was 7,9; 11,3; 18; 30,6 and 33,1 minutes respectively. The low levels of malathion resistance could be conferred by the elevated of α-esterase. The values of the α-esterase in F0, F5, F10, F15 and F20 were 0,155; 0,174; 0,203; 0,209 and 0,215 µmol/min/mg protein respectively. The acetilcholinesterase activities were also raised in F0, F5, F10, F15 and F20, the value of acetilcholinesterase activities were 20,35; 20,26; 23,14; 23,18 and 24,9%.</p>Dwi Jayanti GunandiniP. B. WicaksanaThe Entomological Society of IndonesiaarticleAedes aegypti, malathion, esterase.ZoologyQL1-991ENIDJurnal Entomologi Indonesia, Vol 2, Iss 2 (2017)
institution	DOAJ
collection	DOAJ
language	EN ID
topic	Aedes aegypti, malathion, esterase. Zoology QL1-991
spellingShingle	Aedes aegypti, malathion, esterase. Zoology QL1-991 Dwi Jayanti Gunandini P. B. Wicaksana Peningkatan dan aktivitas enzim asetilkolinesterase pada nyamuk Aedes aegypti yang diseleksi dengan malation
description	<p><strong>The Elevated and Activity of Acetilcholinesterase Enzyme on <em>Aedes aegypti</em> Selected by Malathion.</strong> The aim of this research was to study the effect of selection by malathion on the activity level Acetilcholinesterase enzyme on <em>Aedes aegypti</em> mosquitoes. Selection of <em>Aedes aegypti</em> larval by mean of malathion have been conducted for 20 generations. During the selection process time has been increased of concentration applied and exposure. For generation 0-5 (F0-F5), a concentration of 25 µl/l (24 ppm) was used to expose the larvae to malathion for five minutes. In generation 6-10 (F6-F10) the concentration has increased to 50 µl/l (48 ppm); in F11-F15 the concentration used was 100 µl/l (96 ppm) whereas in F16-F20 200µl/l (192 ppm) was used. Mosquito generations that would be regarded as representative and reference groups were F0, F5, F10, F15 and F20. The LC<sub>50</sub> of F0, F5, F10, F15 and F20 was 0,025; 0,032; 0,042; 0,062 and 0,071 ppm respectively. Increases LT<sub>50</sub> values was also observed in <em>Aedes aegypti</em> selected by malathion. The LT<sub>50</sub> of F0, F5, F10, F15 and F20 generations was 7,9; 11,3; 18; 30,6 and 33,1 minutes respectively. The low levels of malathion resistance could be conferred by the elevated of α-esterase. The values of the α-esterase in F0, F5, F10, F15 and F20 were 0,155; 0,174; 0,203; 0,209 and 0,215 µmol/min/mg protein respectively. The acetilcholinesterase activities were also raised in F0, F5, F10, F15 and F20, the value of acetilcholinesterase activities were 20,35; 20,26; 23,14; 23,18 and 24,9%.</p>
format	article
author	Dwi Jayanti Gunandini P. B. Wicaksana
author_facet	Dwi Jayanti Gunandini P. B. Wicaksana
author_sort	Dwi Jayanti Gunandini
title	Peningkatan dan aktivitas enzim asetilkolinesterase pada nyamuk Aedes aegypti yang diseleksi dengan malation
title_short	Peningkatan dan aktivitas enzim asetilkolinesterase pada nyamuk Aedes aegypti yang diseleksi dengan malation
title_full	Peningkatan dan aktivitas enzim asetilkolinesterase pada nyamuk Aedes aegypti yang diseleksi dengan malation
title_fullStr	Peningkatan dan aktivitas enzim asetilkolinesterase pada nyamuk Aedes aegypti yang diseleksi dengan malation
title_full_unstemmed	Peningkatan dan aktivitas enzim asetilkolinesterase pada nyamuk Aedes aegypti yang diseleksi dengan malation
title_sort	peningkatan dan aktivitas enzim asetilkolinesterase pada nyamuk aedes aegypti yang diseleksi dengan malation
publisher	The Entomological Society of Indonesia
publishDate	2017
url	https://doaj.org/article/49f6ea885421497e80aee3d9ead4fd7b
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Peningkatan dan aktivitas enzim asetilkolinesterase pada nyamuk Aedes aegypti yang diseleksi dengan malation

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