Thyroid hormone deficiency during zebrafish development impairs central nervous system myelination.

Thyroid hormones are messengers that bind to specific nuclear receptors and regulate a wide range of physiological processes in the early stages of vertebrate embryonic development, including neurodevelopment and myelogenesis. We here tested the effects of reduced T3 availability upon the myelinatio...

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Autores principales:	Brenda Minerva Farías-Serratos, Iván Lazcano, Patricia Villalobos, Veerle M Darras, Aurea Orozco
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/49f6facb2dc04d1cbf279d43029d468f
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spelling	oai:doaj.org-article:49f6facb2dc04d1cbf279d43029d468f2021-12-02T20:17:55ZThyroid hormone deficiency during zebrafish development impairs central nervous system myelination.1932-620310.1371/journal.pone.0256207https://doaj.org/article/49f6facb2dc04d1cbf279d43029d468f2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256207https://doaj.org/toc/1932-6203Thyroid hormones are messengers that bind to specific nuclear receptors and regulate a wide range of physiological processes in the early stages of vertebrate embryonic development, including neurodevelopment and myelogenesis. We here tested the effects of reduced T3 availability upon the myelination process by treating zebrafish embryos with low concentrations of iopanoic acid (IOP) to block T4 to T3 conversion. Black Gold II staining showed that T3 deficiency reduced the myelin density in the forebrain, midbrain, hindbrain and the spinal cord at 3 and 7 dpf. These observations were confirmed in 3 dpf mbp:egfp transgenic zebrafish, showing that the administration of IOP reduced the fluorescent signal in the brain. T3 rescue treatment restored brain myelination and reversed the changes in myelin-related gene expression induced by IOP exposure. NG2 immunostaining revealed that T3 deficiency reduced the amount of oligodendrocyte precursor cells in 3 dpf IOP-treated larvae. Altogether, the present results show that inhibition of T4 to T3 conversion results in hypomyelination, suggesting that THs are part of the key signaling molecules that control the timing of oligodendrocyte differentiation and myelin synthesis from very early stages of brain development.Brenda Minerva Farías-SerratosIván LazcanoPatricia VillalobosVeerle M DarrasAurea OrozcoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0256207 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Brenda Minerva Farías-Serratos Iván Lazcano Patricia Villalobos Veerle M Darras Aurea Orozco Thyroid hormone deficiency during zebrafish development impairs central nervous system myelination.
description	Thyroid hormones are messengers that bind to specific nuclear receptors and regulate a wide range of physiological processes in the early stages of vertebrate embryonic development, including neurodevelopment and myelogenesis. We here tested the effects of reduced T3 availability upon the myelination process by treating zebrafish embryos with low concentrations of iopanoic acid (IOP) to block T4 to T3 conversion. Black Gold II staining showed that T3 deficiency reduced the myelin density in the forebrain, midbrain, hindbrain and the spinal cord at 3 and 7 dpf. These observations were confirmed in 3 dpf mbp:egfp transgenic zebrafish, showing that the administration of IOP reduced the fluorescent signal in the brain. T3 rescue treatment restored brain myelination and reversed the changes in myelin-related gene expression induced by IOP exposure. NG2 immunostaining revealed that T3 deficiency reduced the amount of oligodendrocyte precursor cells in 3 dpf IOP-treated larvae. Altogether, the present results show that inhibition of T4 to T3 conversion results in hypomyelination, suggesting that THs are part of the key signaling molecules that control the timing of oligodendrocyte differentiation and myelin synthesis from very early stages of brain development.
format	article
author	Brenda Minerva Farías-Serratos Iván Lazcano Patricia Villalobos Veerle M Darras Aurea Orozco
author_facet	Brenda Minerva Farías-Serratos Iván Lazcano Patricia Villalobos Veerle M Darras Aurea Orozco
author_sort	Brenda Minerva Farías-Serratos
title	Thyroid hormone deficiency during zebrafish development impairs central nervous system myelination.
title_short	Thyroid hormone deficiency during zebrafish development impairs central nervous system myelination.
title_full	Thyroid hormone deficiency during zebrafish development impairs central nervous system myelination.
title_fullStr	Thyroid hormone deficiency during zebrafish development impairs central nervous system myelination.
title_full_unstemmed	Thyroid hormone deficiency during zebrafish development impairs central nervous system myelination.
title_sort	thyroid hormone deficiency during zebrafish development impairs central nervous system myelination.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/49f6facb2dc04d1cbf279d43029d468f
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Thyroid hormone deficiency during zebrafish development impairs central nervous system myelination.

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