Marker-Assisted Evaluation of Two Powdery Mildew Resistance Candidate Genes in Korean Cucumber Inbred Lines
Two genes, <i>CsLRR-RPK2</i> (<i>CsGy5G015660</i>) and <i>CsaMLO8</i> (<i>Csa5G623470</i>), have been considered as powdery mildew (PM) resistance genes in cucumbers. In this study, we evaluated the involvement of the alleles of these two genes in PM r...
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| Autores principales: | , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/49fa6059cf314de195aa71891c0c672e |
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| Sumario: | Two genes, <i>CsLRR-RPK2</i> (<i>CsGy5G015660</i>) and <i>CsaMLO8</i> (<i>Csa5G623470</i>), have been considered as powdery mildew (PM) resistance genes in cucumbers. In this study, we evaluated the involvement of the alleles of these two genes in PM resistance in 100 commercial Korean cucumber inbred lines. To achieve this, we developed cleaved amplified polymorphic sequences (CAPS) and InDel markers from <i>CsLRR-RPK2</i> and <i>CsaMLO8</i>. Genotyping analysis indicated that the <i>CsLRR-RPK2</i>-CAPS marker showed a stronger correlation with the PM-resistant phenotype, with an 84% consistency compared to the <i>CsaMLO8</i>-InDel marker. The use of the <i>CsaMLO8</i>-InDel marker showed a 70% consistency between phenotype and genotype results. It was proposed that the <i>CsLRR-RPK2</i>-CAPS marker successfully eliminated PM-susceptible inbred lines, since both genotype and phenotype results were 100% identical. Furthermore, the present study revealed that the introduction of one of these alleles is probably enough to confer PM resistance in cucumbers. However, seven PM-resistant inbred lines harbored either <i>CsaMLO8</i> or <i>CsLRR-RPK2</i> alleles, indicating that there is another PM-resistant resource(s) besides <i>CsaMLO8-</i> and <i>CsLRR-RPK2</i>–originated resistance in the commercial Korean inbred lines. Our results provide reliable evidence confirming two PM-resistant candidate genes for the detection of PM resistance resources in cucumber inbred lines. |
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