Marker-Assisted Evaluation of Two Powdery Mildew Resistance Candidate Genes in Korean Cucumber Inbred Lines

Marker-Assisted Evaluation of Two Powdery Mildew Resistance Candidate Genes in Korean Cucumber Inbred Lines

Two genes, <i>CsLRR-RPK2</i> (<i>CsGy5G015660</i>) and <i>CsaMLO8</i> (<i>Csa5G623470</i>), have been considered as powdery mildew (PM) resistance genes in cucumbers. In this study, we evaluated the involvement of the alleles of these two genes in PM r...

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Autores principales: Mahdi Badri Anarjan, Ikhyun Bae, Sanghyeob Lee
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
<i>Cucumis sativus</i> L.
<i>Podosphaera xanthii</i>
PM-resistance resource
<i>MLO</i>
<i>LRR-RPK2</i>
Agriculture
S
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spelling oai:doaj.org-article:49fa6059cf314de195aa71891c0c672e2021-11-25T16:06:01ZMarker-Assisted Evaluation of Two Powdery Mildew Resistance Candidate Genes in Korean Cucumber Inbred Lines10.3390/agronomy111121912073-4395https://doaj.org/article/49fa6059cf314de195aa71891c0c672e2021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4395/11/11/2191https://doaj.org/toc/2073-4395Two genes, <i>CsLRR-RPK2</i> (<i>CsGy5G015660</i>) and <i>CsaMLO8</i> (<i>Csa5G623470</i>), have been considered as powdery mildew (PM) resistance genes in cucumbers. In this study, we evaluated the involvement of the alleles of these two genes in PM resistance in 100 commercial Korean cucumber inbred lines. To achieve this, we developed cleaved amplified polymorphic sequences (CAPS) and InDel markers from <i>CsLRR-RPK2</i> and <i>CsaMLO8</i>. Genotyping analysis indicated that the <i>CsLRR-RPK2</i>-CAPS marker showed a stronger correlation with the PM-resistant phenotype, with an 84% consistency compared to the <i>CsaMLO8</i>-InDel marker. The use of the <i>CsaMLO8</i>-InDel marker showed a 70% consistency between phenotype and genotype results. It was proposed that the <i>CsLRR-RPK2</i>-CAPS marker successfully eliminated PM-susceptible inbred lines, since both genotype and phenotype results were 100% identical. Furthermore, the present study revealed that the introduction of one of these alleles is probably enough to confer PM resistance in cucumbers. However, seven PM-resistant inbred lines harbored either <i>CsaMLO8</i> or <i>CsLRR-RPK2</i> alleles, indicating that there is another PM-resistant resource(s) besides <i>CsaMLO8-</i> and <i>CsLRR-RPK2</i>–originated resistance in the commercial Korean inbred lines. Our results provide reliable evidence confirming two PM-resistant candidate genes for the detection of PM resistance resources in cucumber inbred lines.Mahdi Badri AnarjanIkhyun BaeSanghyeob LeeMDPI AGarticle<i>Cucumis sativus</i> L.<i>Podosphaera xanthii</i>PM-resistance resource<i>MLO</i><i>LRR-RPK2</i>AgricultureSENAgronomy, Vol 11, Iss 2191, p 2191 (2021)
institution DOAJ
collection DOAJ
language EN
topic <i>Cucumis sativus</i> L.
<i>Podosphaera xanthii</i>
PM-resistance resource
<i>MLO</i>
<i>LRR-RPK2</i>
Agriculture
S
spellingShingle <i>Cucumis sativus</i> L.
<i>Podosphaera xanthii</i>
PM-resistance resource
<i>MLO</i>
<i>LRR-RPK2</i>
Agriculture
S
Mahdi Badri Anarjan
Ikhyun Bae
Sanghyeob Lee
Marker-Assisted Evaluation of Two Powdery Mildew Resistance Candidate Genes in Korean Cucumber Inbred Lines
description Two genes, <i>CsLRR-RPK2</i> (<i>CsGy5G015660</i>) and <i>CsaMLO8</i> (<i>Csa5G623470</i>), have been considered as powdery mildew (PM) resistance genes in cucumbers. In this study, we evaluated the involvement of the alleles of these two genes in PM resistance in 100 commercial Korean cucumber inbred lines. To achieve this, we developed cleaved amplified polymorphic sequences (CAPS) and InDel markers from <i>CsLRR-RPK2</i> and <i>CsaMLO8</i>. Genotyping analysis indicated that the <i>CsLRR-RPK2</i>-CAPS marker showed a stronger correlation with the PM-resistant phenotype, with an 84% consistency compared to the <i>CsaMLO8</i>-InDel marker. The use of the <i>CsaMLO8</i>-InDel marker showed a 70% consistency between phenotype and genotype results. It was proposed that the <i>CsLRR-RPK2</i>-CAPS marker successfully eliminated PM-susceptible inbred lines, since both genotype and phenotype results were 100% identical. Furthermore, the present study revealed that the introduction of one of these alleles is probably enough to confer PM resistance in cucumbers. However, seven PM-resistant inbred lines harbored either <i>CsaMLO8</i> or <i>CsLRR-RPK2</i> alleles, indicating that there is another PM-resistant resource(s) besides <i>CsaMLO8-</i> and <i>CsLRR-RPK2</i>–originated resistance in the commercial Korean inbred lines. Our results provide reliable evidence confirming two PM-resistant candidate genes for the detection of PM resistance resources in cucumber inbred lines.
format article
author Mahdi Badri Anarjan
Ikhyun Bae
Sanghyeob Lee
author_facet Mahdi Badri Anarjan
Ikhyun Bae
Sanghyeob Lee
author_sort Mahdi Badri Anarjan
title Marker-Assisted Evaluation of Two Powdery Mildew Resistance Candidate Genes in Korean Cucumber Inbred Lines
title_short Marker-Assisted Evaluation of Two Powdery Mildew Resistance Candidate Genes in Korean Cucumber Inbred Lines
title_full Marker-Assisted Evaluation of Two Powdery Mildew Resistance Candidate Genes in Korean Cucumber Inbred Lines
title_fullStr Marker-Assisted Evaluation of Two Powdery Mildew Resistance Candidate Genes in Korean Cucumber Inbred Lines
title_full_unstemmed Marker-Assisted Evaluation of Two Powdery Mildew Resistance Candidate Genes in Korean Cucumber Inbred Lines
title_sort marker-assisted evaluation of two powdery mildew resistance candidate genes in korean cucumber inbred lines
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/49fa6059cf314de195aa71891c0c672e
work_keys_str_mv AT mahdibadrianarjan markerassistedevaluationoftwopowderymildewresistancecandidategenesinkoreancucumberinbredlines
AT ikhyunbae markerassistedevaluationoftwopowderymildewresistancecandidategenesinkoreancucumberinbredlines
AT sanghyeoblee markerassistedevaluationoftwopowderymildewresistancecandidategenesinkoreancucumberinbredlines
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