Self-organization of the Escherichia coli chemotaxis network imaged with super-resolution light microscopy.

The Escherichia coli chemotaxis network is a model system for biological signal processing. In E. coli, transmembrane receptors responsible for signal transduction assemble into large clusters containing several thousand proteins. These sensory clusters have been observed at cell poles and future di...

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Autores principales:	Derek Greenfield, Ann L McEvoy, Hari Shroff, Gavin E Crooks, Ned S Wingreen, Eric Betzig, Jan Liphardt
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/49fd1db97573496b88357e96626b5b57
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spelling	oai:doaj.org-article:49fd1db97573496b88357e96626b5b572021-11-25T05:34:08ZSelf-organization of the Escherichia coli chemotaxis network imaged with super-resolution light microscopy.1544-91731545-788510.1371/journal.pbio.1000137https://doaj.org/article/49fd1db97573496b88357e96626b5b572009-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19547746/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885The Escherichia coli chemotaxis network is a model system for biological signal processing. In E. coli, transmembrane receptors responsible for signal transduction assemble into large clusters containing several thousand proteins. These sensory clusters have been observed at cell poles and future division sites. Despite extensive study, it remains unclear how chemotaxis clusters form, what controls cluster size and density, and how the cellular location of clusters is robustly maintained in growing and dividing cells. Here, we use photoactivated localization microscopy (PALM) to map the cellular locations of three proteins central to bacterial chemotaxis (the Tar receptor, CheY, and CheW) with a precision of 15 nm. We find that cluster sizes are approximately exponentially distributed, with no characteristic cluster size. One-third of Tar receptors are part of smaller lateral clusters and not of the large polar clusters. Analysis of the relative cellular locations of 1.1 million individual proteins (from 326 cells) suggests that clusters form via stochastic self-assembly. The super-resolution PALM maps of E. coli receptors support the notion that stochastic self-assembly can create and maintain approximately periodic structures in biological membranes, without direct cytoskeletal involvement or active transport.Derek GreenfieldAnn L McEvoyHari ShroffGavin E CrooksNed S WingreenEric BetzigJan LiphardtPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 7, Iss 6, p e1000137 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Derek Greenfield Ann L McEvoy Hari Shroff Gavin E Crooks Ned S Wingreen Eric Betzig Jan Liphardt Self-organization of the Escherichia coli chemotaxis network imaged with super-resolution light microscopy.
description	The Escherichia coli chemotaxis network is a model system for biological signal processing. In E. coli, transmembrane receptors responsible for signal transduction assemble into large clusters containing several thousand proteins. These sensory clusters have been observed at cell poles and future division sites. Despite extensive study, it remains unclear how chemotaxis clusters form, what controls cluster size and density, and how the cellular location of clusters is robustly maintained in growing and dividing cells. Here, we use photoactivated localization microscopy (PALM) to map the cellular locations of three proteins central to bacterial chemotaxis (the Tar receptor, CheY, and CheW) with a precision of 15 nm. We find that cluster sizes are approximately exponentially distributed, with no characteristic cluster size. One-third of Tar receptors are part of smaller lateral clusters and not of the large polar clusters. Analysis of the relative cellular locations of 1.1 million individual proteins (from 326 cells) suggests that clusters form via stochastic self-assembly. The super-resolution PALM maps of E. coli receptors support the notion that stochastic self-assembly can create and maintain approximately periodic structures in biological membranes, without direct cytoskeletal involvement or active transport.
format	article
author	Derek Greenfield Ann L McEvoy Hari Shroff Gavin E Crooks Ned S Wingreen Eric Betzig Jan Liphardt
author_facet	Derek Greenfield Ann L McEvoy Hari Shroff Gavin E Crooks Ned S Wingreen Eric Betzig Jan Liphardt
author_sort	Derek Greenfield
title	Self-organization of the Escherichia coli chemotaxis network imaged with super-resolution light microscopy.
title_short	Self-organization of the Escherichia coli chemotaxis network imaged with super-resolution light microscopy.
title_full	Self-organization of the Escherichia coli chemotaxis network imaged with super-resolution light microscopy.
title_fullStr	Self-organization of the Escherichia coli chemotaxis network imaged with super-resolution light microscopy.
title_full_unstemmed	Self-organization of the Escherichia coli chemotaxis network imaged with super-resolution light microscopy.
title_sort	self-organization of the escherichia coli chemotaxis network imaged with super-resolution light microscopy.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/49fd1db97573496b88357e96626b5b57
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Self-organization of the Escherichia coli chemotaxis network imaged with super-resolution light microscopy.

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