Improvement in the amine glass platform by bubbling method for a DNA microarray

Seung Hyun Jee,1 Jong Won Kim,2 Ji Hyeong Lee,2 Young Soo Yoon11Department of Chemical and Biological Engineering, Gachon University, Seongnam, Gyeonggi, Republic of Korea; 2Genomics Clinical Research Institute, LabGenomics Co., Ltd., Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of KoreaAbstract:...

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Autores principales:	Jee SH, Kim JW, Lee JH, Yoon YS
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4a0129ee017b4f0193c9d9a562a5354b
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spelling	oai:doaj.org-article:4a0129ee017b4f0193c9d9a562a5354b2021-12-02T02:29:09ZImprovement in the amine glass platform by bubbling method for a DNA microarray1178-2013https://doaj.org/article/4a0129ee017b4f0193c9d9a562a5354b2015-10-01T00:00:00Zhttps://www.dovepress.com/improvement-in-the-amine-glass-platform-by-bubbling-method-for-a-dna-m-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Seung Hyun Jee,1 Jong Won Kim,2 Ji Hyeong Lee,2 Young Soo Yoon11Department of Chemical and Biological Engineering, Gachon University, Seongnam, Gyeonggi, Republic of Korea; 2Genomics Clinical Research Institute, LabGenomics Co., Ltd., Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of KoreaAbstract: A glass platform with high sensitivity for sexually transmitted diseases microarray is described here. An amino-silane-based self-assembled monolayer was coated on the surface of a glass platform using a novel bubbling method. The optimized surface of the glass platform had highly uniform surface modifications using this method, as well as improved hybridization properties with capture probes in the DNA microarray. On the basis of these results, the improved glass platform serves as a highly reliable and optimal material for the DNA microarray. Moreover, in this study, we demonstrated that our glass platform, manufactured by utilizing the bubbling method, had higher uniformity, shorter processing time, lower background signal, and higher spot signal than the platforms manufactured by the general dipping method. The DNA microarray manufactured with a glass platform prepared using bubbling method can be used as a clinical diagnostic tool. Keywords: DNA microarray, glass platform, bubbling method, self-assambled monolayerJee SHKim JWLee JHYoon YSDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss Special Issue on diverse applications in Nano-Theranostics, Pp 273-280 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Jee SH Kim JW Lee JH Yoon YS Improvement in the amine glass platform by bubbling method for a DNA microarray
description	Seung Hyun Jee,1 Jong Won Kim,2 Ji Hyeong Lee,2 Young Soo Yoon11Department of Chemical and Biological Engineering, Gachon University, Seongnam, Gyeonggi, Republic of Korea; 2Genomics Clinical Research Institute, LabGenomics Co., Ltd., Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of KoreaAbstract: A glass platform with high sensitivity for sexually transmitted diseases microarray is described here. An amino-silane-based self-assembled monolayer was coated on the surface of a glass platform using a novel bubbling method. The optimized surface of the glass platform had highly uniform surface modifications using this method, as well as improved hybridization properties with capture probes in the DNA microarray. On the basis of these results, the improved glass platform serves as a highly reliable and optimal material for the DNA microarray. Moreover, in this study, we demonstrated that our glass platform, manufactured by utilizing the bubbling method, had higher uniformity, shorter processing time, lower background signal, and higher spot signal than the platforms manufactured by the general dipping method. The DNA microarray manufactured with a glass platform prepared using bubbling method can be used as a clinical diagnostic tool. Keywords: DNA microarray, glass platform, bubbling method, self-assambled monolayer
format	article
author	Jee SH Kim JW Lee JH Yoon YS
author_facet	Jee SH Kim JW Lee JH Yoon YS
author_sort	Jee SH
title	Improvement in the amine glass platform by bubbling method for a DNA microarray
title_short	Improvement in the amine glass platform by bubbling method for a DNA microarray
title_full	Improvement in the amine glass platform by bubbling method for a DNA microarray
title_fullStr	Improvement in the amine glass platform by bubbling method for a DNA microarray
title_full_unstemmed	Improvement in the amine glass platform by bubbling method for a DNA microarray
title_sort	improvement in the amine glass platform by bubbling method for a dna microarray
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/4a0129ee017b4f0193c9d9a562a5354b
work_keys_str_mv	AT jeesh improvementintheamineglassplatformbybubblingmethodforadnamicroarray AT kimjw improvementintheamineglassplatformbybubblingmethodforadnamicroarray AT leejh improvementintheamineglassplatformbybubblingmethodforadnamicroarray AT yoonys improvementintheamineglassplatformbybubblingmethodforadnamicroarray
_version_	1718402464450871296

Improvement in the amine glass platform by bubbling method for a DNA microarray

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