Mutation of Gyra Gene Found In Mycobacterium Leprae From Leprosy Patient In West Papua and Papua, Indonesia

Cases of leprosy in Indonesia are still high, especially in the provinces of West Papua, North Maluku and Papua. Drug resistance surveillance and typing strains of Mycobacterium leprae are useful molecular tools for leprosy control especially in the three Provinces. The purpose of this study was to...

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Autores principales:	Yustinus Maladan, Hana Krismawati, Rosana Agus, Hotma M.L. Hutapea, Ratna Tanjung, Vatim Dwi Cahyani, Muhammad Fajri Rokhmad, Arli Aditya Parikesit
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Gadjah Mada 2021
Materias:	leprosy gyra ofloxacin mycobacterium leprae Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/4a01f091588049e38032e827c80784f3
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spelling	oai:doaj.org-article:4a01f091588049e38032e827c80784f32021-11-15T06:08:47ZMutation of Gyra Gene Found In Mycobacterium Leprae From Leprosy Patient In West Papua and Papua, Indonesia2338-94272338-948610.22146/ijp.848https://doaj.org/article/4a01f091588049e38032e827c80784f32021-01-01T00:00:00Zhttps://jurnal.ugm.ac.id/v3/IJP/article/view/848https://doaj.org/toc/2338-9427https://doaj.org/toc/2338-9486Cases of leprosy in Indonesia are still high, especially in the provinces of West Papua, North Maluku and Papua. Drug resistance surveillance and typing strains of Mycobacterium leprae are useful molecular tools for leprosy control especially in the three Provinces. The purpose of this study was to identify mutations in the gyrA M. leprae gene obtained from leprosy patients in the provinces of West Papua and Papua on a molecular basis. M. leprae samples obtained from leprosy patients were extracted and continued with PCR and sequencing in the M. leprae gyrA gene. The sequencing results are aligned with M. leprae TN sequences to identify mutations. The phylogenetic tree was constructed using Mega 7 to get the M. leprae gyrA cluster. The RNAalifold server was employed to generate the conserved 2D structure for the gyrA MSAs. Six variants were found in the gyrA M. leprae obtained from the provinces of West Papua and Papua. The six variants are H71R, K73R, D95G, A101T, R107W, A127V. The existence of mutations in the gyrA M. leprae gene found in this study can be information in the treatment of leprosy in Papua if using Ofloxacin as an alternative treatment. Based on phylogenetic analysis found there are three distinct clusters of gyrA gene. The five variants are H71R, K73R, A101T, R107W, A127V are new variant of gyrA M. leprae. The D95G variant has been confirmed to cause resistance to Fluoroquinolone by in vitro methods, while the H71R, K73R, A101T, R107W, A127V variants are new variants whose effects on the fluoroquinolone are unknown. Thus, further analysis is needed to study the effects of the five variants on ofloxacin.Yustinus MaladanHana KrismawatiRosana AgusHotma M.L. HutapeaRatna TanjungVatim Dwi CahyaniMuhammad Fajri RokhmadArli Aditya ParikesitUniversitas Gadjah Madaarticleleprosygyraofloxacinmycobacterium lepraePharmacy and materia medicaRS1-441ENIndonesian Journal of Pharmacy, Pp 35-42 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	leprosy gyra ofloxacin mycobacterium leprae Pharmacy and materia medica RS1-441
spellingShingle	leprosy gyra ofloxacin mycobacterium leprae Pharmacy and materia medica RS1-441 Yustinus Maladan Hana Krismawati Rosana Agus Hotma M.L. Hutapea Ratna Tanjung Vatim Dwi Cahyani Muhammad Fajri Rokhmad Arli Aditya Parikesit Mutation of Gyra Gene Found In Mycobacterium Leprae From Leprosy Patient In West Papua and Papua, Indonesia
description	Cases of leprosy in Indonesia are still high, especially in the provinces of West Papua, North Maluku and Papua. Drug resistance surveillance and typing strains of Mycobacterium leprae are useful molecular tools for leprosy control especially in the three Provinces. The purpose of this study was to identify mutations in the gyrA M. leprae gene obtained from leprosy patients in the provinces of West Papua and Papua on a molecular basis. M. leprae samples obtained from leprosy patients were extracted and continued with PCR and sequencing in the M. leprae gyrA gene. The sequencing results are aligned with M. leprae TN sequences to identify mutations. The phylogenetic tree was constructed using Mega 7 to get the M. leprae gyrA cluster. The RNAalifold server was employed to generate the conserved 2D structure for the gyrA MSAs. Six variants were found in the gyrA M. leprae obtained from the provinces of West Papua and Papua. The six variants are H71R, K73R, D95G, A101T, R107W, A127V. The existence of mutations in the gyrA M. leprae gene found in this study can be information in the treatment of leprosy in Papua if using Ofloxacin as an alternative treatment. Based on phylogenetic analysis found there are three distinct clusters of gyrA gene. The five variants are H71R, K73R, A101T, R107W, A127V are new variant of gyrA M. leprae. The D95G variant has been confirmed to cause resistance to Fluoroquinolone by in vitro methods, while the H71R, K73R, A101T, R107W, A127V variants are new variants whose effects on the fluoroquinolone are unknown. Thus, further analysis is needed to study the effects of the five variants on ofloxacin.
format	article
author	Yustinus Maladan Hana Krismawati Rosana Agus Hotma M.L. Hutapea Ratna Tanjung Vatim Dwi Cahyani Muhammad Fajri Rokhmad Arli Aditya Parikesit
author_facet	Yustinus Maladan Hana Krismawati Rosana Agus Hotma M.L. Hutapea Ratna Tanjung Vatim Dwi Cahyani Muhammad Fajri Rokhmad Arli Aditya Parikesit
author_sort	Yustinus Maladan
title	Mutation of Gyra Gene Found In Mycobacterium Leprae From Leprosy Patient In West Papua and Papua, Indonesia
title_short	Mutation of Gyra Gene Found In Mycobacterium Leprae From Leprosy Patient In West Papua and Papua, Indonesia
title_full	Mutation of Gyra Gene Found In Mycobacterium Leprae From Leprosy Patient In West Papua and Papua, Indonesia
title_fullStr	Mutation of Gyra Gene Found In Mycobacterium Leprae From Leprosy Patient In West Papua and Papua, Indonesia
title_full_unstemmed	Mutation of Gyra Gene Found In Mycobacterium Leprae From Leprosy Patient In West Papua and Papua, Indonesia
title_sort	mutation of gyra gene found in mycobacterium leprae from leprosy patient in west papua and papua, indonesia
publisher	Universitas Gadjah Mada
publishDate	2021
url	https://doaj.org/article/4a01f091588049e38032e827c80784f3
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Mutation of Gyra Gene Found In Mycobacterium Leprae From Leprosy Patient In West Papua and Papua, Indonesia

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