In vitro cytotoxic potential of extracts from Aristolochia foetida Kunth against MCF-7 and bMECs cell lines

The aim of this study was to evaluate the cytotoxic potential of Aristolochia foetida Kunth. Stems and leaves of A. foetida Kunth (Aristolochiaceae) have never been investigated pharmacologically. Recent studies of species of the Aristolochiaceae family found significant cytotoxic activities. Hexane...

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Autores principales:	Martín A. Lerma-Herrera, Lidia Beiza-Granados, Alejandra Ochoa-Zarzosa, Joel E. López-Meza, Juan D. Hernández-Hernández, Judit Aviña-Verduzco, Hugo A. García-Gutiérrez
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Apoptosis Aristolochia foetida Cytotoxicity Flow cytometry MCF-7 breast cancer cell Medicinal plants Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/4a057ac86505460e90a8301b2d95388e
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spelling	oai:doaj.org-article:4a057ac86505460e90a8301b2d95388e2021-11-20T04:56:46ZIn vitro cytotoxic potential of extracts from Aristolochia foetida Kunth against MCF-7 and bMECs cell lines1319-562X10.1016/j.sjbs.2021.08.007https://doaj.org/article/4a057ac86505460e90a8301b2d95388e2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1319562X2100680Xhttps://doaj.org/toc/1319-562XThe aim of this study was to evaluate the cytotoxic potential of Aristolochia foetida Kunth. Stems and leaves of A. foetida Kunth (Aristolochiaceae) have never been investigated pharmacologically. Recent studies of species of the Aristolochiaceae family found significant cytotoxic activities. Hexane, dichloromethane, ethyl acetate and methanol extracts were analyzed by 1H NMR and GC–MS to know the metabolites in each extract. In GC–MS analysis, the main compounds were methyl hexadecanoate (3); hexadecanoic acid (4); 2-butoxyethyl dodecanoate (9); ethyl hexadecanoate (20); methyl octadeca-9,12,15-trienoate (28) and (9Z,12Z,15Z)-octadeca-9,12,15-trienoic acid (40). The results showed a significant reduction in cell viability of the MCF-7 (breast cancer) cell line caused by organic extracts in a dose-dependent manner. The cytotoxicity activity of the dichloromethane extract from the stems (DSE) showed IC50 values of 45.9 μg/mL and the dichloromethane extract of the leaves (DLE) showed IC50 values of 47.3 μg/mL. DSE and DLE had the highest cytotoxic potential in an in vitro study against the MCF-7 cell line and non-tumor cells obtained from the bovine mammary epithelial (bMECs). DSE and DLE induced a loss in mitochondrial membrane potential (ΔΨm) and can cause cell death by apoptosis through the intrinsic pathway in the MCF-7 cell line. DSE and DLE are cytotoxic in cancer cells and cause late apoptosis. Higher concentrations of DSE and DLE are required to induce a cytotoxic effect in healthy mammary epithelial cells. This is the first report of the dichloromethane extract of A. foetida Kunth that induces late apoptosis in MCF-7 cancer cells and may be a candidate for pharmacological study against breast cancer.Martín A. Lerma-HerreraLidia Beiza-GranadosAlejandra Ochoa-ZarzosaJoel E. López-MezaJuan D. Hernández-HernándezJudit Aviña-VerduzcoHugo A. García-GutiérrezElsevierarticleApoptosisAristolochia foetidaCytotoxicityFlow cytometryMCF-7 breast cancer cellMedicinal plantsBiology (General)QH301-705.5ENSaudi Journal of Biological Sciences, Vol 28, Iss 12, Pp 7082-7089 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Apoptosis Aristolochia foetida Cytotoxicity Flow cytometry MCF-7 breast cancer cell Medicinal plants Biology (General) QH301-705.5
spellingShingle	Apoptosis Aristolochia foetida Cytotoxicity Flow cytometry MCF-7 breast cancer cell Medicinal plants Biology (General) QH301-705.5 Martín A. Lerma-Herrera Lidia Beiza-Granados Alejandra Ochoa-Zarzosa Joel E. López-Meza Juan D. Hernández-Hernández Judit Aviña-Verduzco Hugo A. García-Gutiérrez In vitro cytotoxic potential of extracts from Aristolochia foetida Kunth against MCF-7 and bMECs cell lines
description	The aim of this study was to evaluate the cytotoxic potential of Aristolochia foetida Kunth. Stems and leaves of A. foetida Kunth (Aristolochiaceae) have never been investigated pharmacologically. Recent studies of species of the Aristolochiaceae family found significant cytotoxic activities. Hexane, dichloromethane, ethyl acetate and methanol extracts were analyzed by 1H NMR and GC–MS to know the metabolites in each extract. In GC–MS analysis, the main compounds were methyl hexadecanoate (3); hexadecanoic acid (4); 2-butoxyethyl dodecanoate (9); ethyl hexadecanoate (20); methyl octadeca-9,12,15-trienoate (28) and (9Z,12Z,15Z)-octadeca-9,12,15-trienoic acid (40). The results showed a significant reduction in cell viability of the MCF-7 (breast cancer) cell line caused by organic extracts in a dose-dependent manner. The cytotoxicity activity of the dichloromethane extract from the stems (DSE) showed IC50 values of 45.9 μg/mL and the dichloromethane extract of the leaves (DLE) showed IC50 values of 47.3 μg/mL. DSE and DLE had the highest cytotoxic potential in an in vitro study against the MCF-7 cell line and non-tumor cells obtained from the bovine mammary epithelial (bMECs). DSE and DLE induced a loss in mitochondrial membrane potential (ΔΨm) and can cause cell death by apoptosis through the intrinsic pathway in the MCF-7 cell line. DSE and DLE are cytotoxic in cancer cells and cause late apoptosis. Higher concentrations of DSE and DLE are required to induce a cytotoxic effect in healthy mammary epithelial cells. This is the first report of the dichloromethane extract of A. foetida Kunth that induces late apoptosis in MCF-7 cancer cells and may be a candidate for pharmacological study against breast cancer.
format	article
author	Martín A. Lerma-Herrera Lidia Beiza-Granados Alejandra Ochoa-Zarzosa Joel E. López-Meza Juan D. Hernández-Hernández Judit Aviña-Verduzco Hugo A. García-Gutiérrez
author_facet	Martín A. Lerma-Herrera Lidia Beiza-Granados Alejandra Ochoa-Zarzosa Joel E. López-Meza Juan D. Hernández-Hernández Judit Aviña-Verduzco Hugo A. García-Gutiérrez
author_sort	Martín A. Lerma-Herrera
title	In vitro cytotoxic potential of extracts from Aristolochia foetida Kunth against MCF-7 and bMECs cell lines
title_short	In vitro cytotoxic potential of extracts from Aristolochia foetida Kunth against MCF-7 and bMECs cell lines
title_full	In vitro cytotoxic potential of extracts from Aristolochia foetida Kunth against MCF-7 and bMECs cell lines
title_fullStr	In vitro cytotoxic potential of extracts from Aristolochia foetida Kunth against MCF-7 and bMECs cell lines
title_full_unstemmed	In vitro cytotoxic potential of extracts from Aristolochia foetida Kunth against MCF-7 and bMECs cell lines
title_sort	in vitro cytotoxic potential of extracts from aristolochia foetida kunth against mcf-7 and bmecs cell lines
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/4a057ac86505460e90a8301b2d95388e
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In vitro cytotoxic potential of extracts from Aristolochia foetida Kunth against MCF-7 and bMECs cell lines

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