Sensitive and powerful single-cell RNA sequencing using mcSCRB-seq

Single-cell RNA-barcoding and sequencing is an efficient, genome-wide method to characterize cellular identities. Here the authors systematically evaluate the protocol and develop molecular crowding SCRB-seq with improved sensitivity and cost-efficiency.

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Autores principales: Johannes W. Bagnoli, Christoph Ziegenhain, Aleksandar Janjic, Lucas E. Wange, Beate Vieth, Swati Parekh, Johanna Geuder, Ines Hellmann, Wolfgang Enard
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/4a131646ab114fc18a5468c079161e41
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spelling oai:doaj.org-article:4a131646ab114fc18a5468c079161e412021-12-02T15:34:50ZSensitive and powerful single-cell RNA sequencing using mcSCRB-seq10.1038/s41467-018-05347-62041-1723https://doaj.org/article/4a131646ab114fc18a5468c079161e412018-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-05347-6https://doaj.org/toc/2041-1723Single-cell RNA-barcoding and sequencing is an efficient, genome-wide method to characterize cellular identities. Here the authors systematically evaluate the protocol and develop molecular crowding SCRB-seq with improved sensitivity and cost-efficiency.Johannes W. BagnoliChristoph ZiegenhainAleksandar JanjicLucas E. WangeBeate ViethSwati ParekhJohanna GeuderInes HellmannWolfgang EnardNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-8 (2018)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Johannes W. Bagnoli
Christoph Ziegenhain
Aleksandar Janjic
Lucas E. Wange
Beate Vieth
Swati Parekh
Johanna Geuder
Ines Hellmann
Wolfgang Enard
Sensitive and powerful single-cell RNA sequencing using mcSCRB-seq
description Single-cell RNA-barcoding and sequencing is an efficient, genome-wide method to characterize cellular identities. Here the authors systematically evaluate the protocol and develop molecular crowding SCRB-seq with improved sensitivity and cost-efficiency.
format article
author Johannes W. Bagnoli
Christoph Ziegenhain
Aleksandar Janjic
Lucas E. Wange
Beate Vieth
Swati Parekh
Johanna Geuder
Ines Hellmann
Wolfgang Enard
author_facet Johannes W. Bagnoli
Christoph Ziegenhain
Aleksandar Janjic
Lucas E. Wange
Beate Vieth
Swati Parekh
Johanna Geuder
Ines Hellmann
Wolfgang Enard
author_sort Johannes W. Bagnoli
title Sensitive and powerful single-cell RNA sequencing using mcSCRB-seq
title_short Sensitive and powerful single-cell RNA sequencing using mcSCRB-seq
title_full Sensitive and powerful single-cell RNA sequencing using mcSCRB-seq
title_fullStr Sensitive and powerful single-cell RNA sequencing using mcSCRB-seq
title_full_unstemmed Sensitive and powerful single-cell RNA sequencing using mcSCRB-seq
title_sort sensitive and powerful single-cell rna sequencing using mcscrb-seq
publisher Nature Portfolio
publishDate 2018
url https://doaj.org/article/4a131646ab114fc18a5468c079161e41
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