Sensitive and powerful single-cell RNA sequencing using mcSCRB-seq

Single-cell RNA-barcoding and sequencing is an efficient, genome-wide method to characterize cellular identities. Here the authors systematically evaluate the protocol and develop molecular crowding SCRB-seq with improved sensitivity and cost-efficiency.

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Autores principales:	Johannes W. Bagnoli, Christoph Ziegenhain, Aleksandar Janjic, Lucas E. Wange, Beate Vieth, Swati Parekh, Johanna Geuder, Ines Hellmann, Wolfgang Enard
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/4a131646ab114fc18a5468c079161e41
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spelling	oai:doaj.org-article:4a131646ab114fc18a5468c079161e412021-12-02T15:34:50ZSensitive and powerful single-cell RNA sequencing using mcSCRB-seq10.1038/s41467-018-05347-62041-1723https://doaj.org/article/4a131646ab114fc18a5468c079161e412018-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-05347-6https://doaj.org/toc/2041-1723Single-cell RNA-barcoding and sequencing is an efficient, genome-wide method to characterize cellular identities. Here the authors systematically evaluate the protocol and develop molecular crowding SCRB-seq with improved sensitivity and cost-efficiency.Johannes W. BagnoliChristoph ZiegenhainAleksandar JanjicLucas E. WangeBeate ViethSwati ParekhJohanna GeuderInes HellmannWolfgang EnardNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Johannes W. Bagnoli Christoph Ziegenhain Aleksandar Janjic Lucas E. Wange Beate Vieth Swati Parekh Johanna Geuder Ines Hellmann Wolfgang Enard Sensitive and powerful single-cell RNA sequencing using mcSCRB-seq
description	Single-cell RNA-barcoding and sequencing is an efficient, genome-wide method to characterize cellular identities. Here the authors systematically evaluate the protocol and develop molecular crowding SCRB-seq with improved sensitivity and cost-efficiency.
format	article
author	Johannes W. Bagnoli Christoph Ziegenhain Aleksandar Janjic Lucas E. Wange Beate Vieth Swati Parekh Johanna Geuder Ines Hellmann Wolfgang Enard
author_facet	Johannes W. Bagnoli Christoph Ziegenhain Aleksandar Janjic Lucas E. Wange Beate Vieth Swati Parekh Johanna Geuder Ines Hellmann Wolfgang Enard
author_sort	Johannes W. Bagnoli
title	Sensitive and powerful single-cell RNA sequencing using mcSCRB-seq
title_short	Sensitive and powerful single-cell RNA sequencing using mcSCRB-seq
title_full	Sensitive and powerful single-cell RNA sequencing using mcSCRB-seq
title_fullStr	Sensitive and powerful single-cell RNA sequencing using mcSCRB-seq
title_full_unstemmed	Sensitive and powerful single-cell RNA sequencing using mcSCRB-seq
title_sort	sensitive and powerful single-cell rna sequencing using mcscrb-seq
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/4a131646ab114fc18a5468c079161e41
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Sensitive and powerful single-cell RNA sequencing using mcSCRB-seq

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