Who's Your DadA? <sc>d</sc>-Alanine Levels Regulate Bacterial Stiffness

ABSTRACT A central question in mechanobiology is how cellular-scale structures are established and regulated. In bacteria, the cell envelope is essential for mechanical integrity, protecting against environmental stresses and bearing the load from high turgor pressures. Trivedi et al. (mBio 9:e01340...

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Autores principales:	Pascal D. Odermatt, Heidi A. Arjes, Fred Chang, Kerwyn Casey Huang
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Pseudomonas high-throughput screening mechanical genomics Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/4a151efb753b4e788cfd50525763b93c
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spelling	oai:doaj.org-article:4a151efb753b4e788cfd50525763b93c2021-11-15T15:58:21ZWho's Your DadA? <sc>d</sc>-Alanine Levels Regulate Bacterial Stiffness10.1128/mBio.02127-182150-7511https://doaj.org/article/4a151efb753b4e788cfd50525763b93c2018-11-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.02127-18https://doaj.org/toc/2150-7511ABSTRACT A central question in mechanobiology is how cellular-scale structures are established and regulated. In bacteria, the cell envelope is essential for mechanical integrity, protecting against environmental stresses and bearing the load from high turgor pressures. Trivedi et al. (mBio 9:e01340-18, 2018, https://doi.org/10.1128/mBio.01340-18) screened a Pseudomonas aeruginosa transposon library and identified genes that influence cell stiffness by measuring cell growth while cells were embedded in an agarose gel. Their findings provide a broad knowledge base for how biochemical pathways regulate cellular mechanical properties in this pathogen. Dozens of genes across diverse functional categories were implicated, suggesting that cellular mechanics is a systems-level emergent property. Furthermore, changes in d-alanine levels in a dadA (d-alanine dehydrogenase) mutant resulted in decreases in the expression of cell wall enzymes, cross-linking density, and cell stiffness. These insights into the biochemical and mechanical roles of dadA highlight the importance of systems-level investigations into the physical properties of cells.Pascal D. OdermattHeidi A. ArjesFred ChangKerwyn Casey HuangAmerican Society for MicrobiologyarticlePseudomonashigh-throughput screeningmechanical genomicsMicrobiologyQR1-502ENmBio, Vol 9, Iss 5 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pseudomonas high-throughput screening mechanical genomics Microbiology QR1-502
spellingShingle	Pseudomonas high-throughput screening mechanical genomics Microbiology QR1-502 Pascal D. Odermatt Heidi A. Arjes Fred Chang Kerwyn Casey Huang Who's Your DadA? <sc>d</sc>-Alanine Levels Regulate Bacterial Stiffness
description	ABSTRACT A central question in mechanobiology is how cellular-scale structures are established and regulated. In bacteria, the cell envelope is essential for mechanical integrity, protecting against environmental stresses and bearing the load from high turgor pressures. Trivedi et al. (mBio 9:e01340-18, 2018, https://doi.org/10.1128/mBio.01340-18) screened a Pseudomonas aeruginosa transposon library and identified genes that influence cell stiffness by measuring cell growth while cells were embedded in an agarose gel. Their findings provide a broad knowledge base for how biochemical pathways regulate cellular mechanical properties in this pathogen. Dozens of genes across diverse functional categories were implicated, suggesting that cellular mechanics is a systems-level emergent property. Furthermore, changes in d-alanine levels in a dadA (d-alanine dehydrogenase) mutant resulted in decreases in the expression of cell wall enzymes, cross-linking density, and cell stiffness. These insights into the biochemical and mechanical roles of dadA highlight the importance of systems-level investigations into the physical properties of cells.
format	article
author	Pascal D. Odermatt Heidi A. Arjes Fred Chang Kerwyn Casey Huang
author_facet	Pascal D. Odermatt Heidi A. Arjes Fred Chang Kerwyn Casey Huang
author_sort	Pascal D. Odermatt
title	Who's Your DadA? <sc>d</sc>-Alanine Levels Regulate Bacterial Stiffness
title_short	Who's Your DadA? <sc>d</sc>-Alanine Levels Regulate Bacterial Stiffness
title_full	Who's Your DadA? <sc>d</sc>-Alanine Levels Regulate Bacterial Stiffness
title_fullStr	Who's Your DadA? <sc>d</sc>-Alanine Levels Regulate Bacterial Stiffness
title_full_unstemmed	Who's Your DadA? <sc>d</sc>-Alanine Levels Regulate Bacterial Stiffness
title_sort	who's your dada? <sc>d</sc>-alanine levels regulate bacterial stiffness
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/4a151efb753b4e788cfd50525763b93c
work_keys_str_mv	AT pascaldodermatt whosyourdadascdscalaninelevelsregulatebacterialstiffness AT heidiaarjes whosyourdadascdscalaninelevelsregulatebacterialstiffness AT fredchang whosyourdadascdscalaninelevelsregulatebacterialstiffness AT kerwyncaseyhuang whosyourdadascdscalaninelevelsregulatebacterialstiffness
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Who's Your DadA? <sc>d</sc>-Alanine Levels Regulate Bacterial Stiffness

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