CD3-positive B cells: a storage-dependent phenomenon.

The majority of clinical studies requires extensive management of human specimen including e.g. overnight shipping of blood samples in order to convey the samples in a central laboratory or to simultaneously analyze large numbers of patients. Storage of blood samples for periods of time before in vi...

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Autores principales:	Angela Nagel, Christian Möbs, Hartmann Raifer, Heinz Wiendl, Michael Hertl, Rüdiger Eming
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4a1910742b8e42e6b75281212224e39a
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spelling	oai:doaj.org-article:4a1910742b8e42e6b75281212224e39a2021-11-25T05:56:24ZCD3-positive B cells: a storage-dependent phenomenon.1932-620310.1371/journal.pone.0110138https://doaj.org/article/4a1910742b8e42e6b75281212224e39a2014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0110138https://doaj.org/toc/1932-6203The majority of clinical studies requires extensive management of human specimen including e.g. overnight shipping of blood samples in order to convey the samples in a central laboratory or to simultaneously analyze large numbers of patients. Storage of blood samples for periods of time before in vitro/ex vivo testing is known to influence the antigen expression on the surface of lymphocytes. In this context, the present results show for the first time that the T cell antigen CD3 can be substantially detected on the surface of human B cells after ex vivo storage and that the degree of this phenomenon critically depends on temperature and duration after blood withdrawal. The appearance of CD3 on the B cell surface seems to be a result of contact-dependent antigen exchange between T and B lymphocytes and is not attributed to endogenous production by B cells. Since cellular subsets are often classified by phenotypic analyses, our results indicate that ex vivo cellular classification in peripheral blood might result in misleading interpretations. Therefore, in order to obtain results reflecting the in vivo situation, it is suggested to minimize times of ex vivo blood storage after isolation of PBMC. Moreover, to enable reproducibility of results between different research groups and multicenter studies, we would emphasize the necessity to specify and standardize the storage conditions, which might be the basis of particular findings.Angela NagelChristian MöbsHartmann RaiferHeinz WiendlMichael HertlRüdiger EmingPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e110138 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Angela Nagel Christian Möbs Hartmann Raifer Heinz Wiendl Michael Hertl Rüdiger Eming CD3-positive B cells: a storage-dependent phenomenon.
description	The majority of clinical studies requires extensive management of human specimen including e.g. overnight shipping of blood samples in order to convey the samples in a central laboratory or to simultaneously analyze large numbers of patients. Storage of blood samples for periods of time before in vitro/ex vivo testing is known to influence the antigen expression on the surface of lymphocytes. In this context, the present results show for the first time that the T cell antigen CD3 can be substantially detected on the surface of human B cells after ex vivo storage and that the degree of this phenomenon critically depends on temperature and duration after blood withdrawal. The appearance of CD3 on the B cell surface seems to be a result of contact-dependent antigen exchange between T and B lymphocytes and is not attributed to endogenous production by B cells. Since cellular subsets are often classified by phenotypic analyses, our results indicate that ex vivo cellular classification in peripheral blood might result in misleading interpretations. Therefore, in order to obtain results reflecting the in vivo situation, it is suggested to minimize times of ex vivo blood storage after isolation of PBMC. Moreover, to enable reproducibility of results between different research groups and multicenter studies, we would emphasize the necessity to specify and standardize the storage conditions, which might be the basis of particular findings.
format	article
author	Angela Nagel Christian Möbs Hartmann Raifer Heinz Wiendl Michael Hertl Rüdiger Eming
author_facet	Angela Nagel Christian Möbs Hartmann Raifer Heinz Wiendl Michael Hertl Rüdiger Eming
author_sort	Angela Nagel
title	CD3-positive B cells: a storage-dependent phenomenon.
title_short	CD3-positive B cells: a storage-dependent phenomenon.
title_full	CD3-positive B cells: a storage-dependent phenomenon.
title_fullStr	CD3-positive B cells: a storage-dependent phenomenon.
title_full_unstemmed	CD3-positive B cells: a storage-dependent phenomenon.
title_sort	cd3-positive b cells: a storage-dependent phenomenon.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/4a1910742b8e42e6b75281212224e39a
work_keys_str_mv	AT angelanagel cd3positivebcellsastoragedependentphenomenon AT christianmobs cd3positivebcellsastoragedependentphenomenon AT hartmannraifer cd3positivebcellsastoragedependentphenomenon AT heinzwiendl cd3positivebcellsastoragedependentphenomenon AT michaelhertl cd3positivebcellsastoragedependentphenomenon AT rudigereming cd3positivebcellsastoragedependentphenomenon
_version_	1718414335270715392

CD3-positive B cells: a storage-dependent phenomenon.

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