Enhanced dermal delivery of diflucortolone valerate using lecithin/chitosan nanoparticles: in-vitro and in-vivo evaluations

İpek &Ouml;zcan, Erkan Azizoğlu, Taner Şenyiğit, Mine &Ouml;zyazıcı, &Ouml;zgen &Ouml;zerEge University, Faculty of Pharmacy, Department of Pharmaceutical Technology, Bornova, Izmir, TurkeyAbstract: The objective of this study was to prepare a suitable formulation for...

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Autores principales:	&Ouml;zcan İ, Azizoğlu E, Şenyiğit T, &Ouml;zyazıcı M, &Ouml;zer &Ouml;
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4a1c4cdf070a489ea4ec5b4a07660cda
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spelling	oai:doaj.org-article:4a1c4cdf070a489ea4ec5b4a07660cda2021-12-02T07:45:07ZEnhanced dermal delivery of diflucortolone valerate using lecithin/chitosan nanoparticles: in-vitro and in-vivo evaluations1176-91141178-2013https://doaj.org/article/4a1c4cdf070a489ea4ec5b4a07660cda2013-01-01T00:00:00Zhttp://www.dovepress.com/enhanced-dermal-delivery-of-diflucortolone-valerate-using-lecithinchit-a12082https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013İpek &Ouml;zcan, Erkan Azizoğlu, Taner Şenyiğit, Mine &Ouml;zyazıcı, &Ouml;zgen &Ouml;zerEge University, Faculty of Pharmacy, Department of Pharmaceutical Technology, Bornova, Izmir, TurkeyAbstract: The objective of this study was to prepare a suitable formulation for dermal delivery of diflucortolone valerate (DFV) that would maintain the localization in skin layers without any penetration and to optimize efficiency of DFV. Drug-loaded lecithin/chitosan nanoparticles with high entrapment efficiency (86.8%), were successfully prepared by ionic interaction technique. Sustained release of DFV was achieved without any initial burst release. Nanoparticles were also incorporated into chitosan gel at different ratios for preparing a more suitable formulation for topical drug delivery with adequate viscosity. In ex-vivo permeation studies, nanoparticles increased the accumulation of DFV especially in the stratum corneum + epidermis of rat skin without any significant permeation. Retention of DFV from nanoparticle in chitosan gel formulation (0.01%) was twofold higher than commercial cream, although it contained ten times less DFV. Nanoparticles in gel formulations produced significantly higher edema inhibition in rats compared with commercial cream in in-vivo studies. Skin blanching assay using a chromameter showed vasoconstriction similar to that of the commercial product. There were no barrier function changes upon application of nanoparticles. In-vitro and in-vivo results demonstrated that lecithin/chitosan nanoparticles in chitosan gel may be a promising carrier for dermal delivery of DFV in various skin disorders.Keywords: skin permeation, anti-inflammatory activity, skin blanching, TEWL&Ouml;zcan İAzizoğlu EŞenyiğit T&Ouml;zyazıcı M&Ouml;zer &Ouml;Dove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 461-475 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 &Ouml;zcan İ Azizoğlu E Şenyiğit T &Ouml;zyazıcı M &Ouml;zer &Ouml; Enhanced dermal delivery of diflucortolone valerate using lecithin/chitosan nanoparticles: in-vitro and in-vivo evaluations
description	İpek &Ouml;zcan, Erkan Azizoğlu, Taner Şenyiğit, Mine &Ouml;zyazıcı, &Ouml;zgen &Ouml;zerEge University, Faculty of Pharmacy, Department of Pharmaceutical Technology, Bornova, Izmir, TurkeyAbstract: The objective of this study was to prepare a suitable formulation for dermal delivery of diflucortolone valerate (DFV) that would maintain the localization in skin layers without any penetration and to optimize efficiency of DFV. Drug-loaded lecithin/chitosan nanoparticles with high entrapment efficiency (86.8%), were successfully prepared by ionic interaction technique. Sustained release of DFV was achieved without any initial burst release. Nanoparticles were also incorporated into chitosan gel at different ratios for preparing a more suitable formulation for topical drug delivery with adequate viscosity. In ex-vivo permeation studies, nanoparticles increased the accumulation of DFV especially in the stratum corneum + epidermis of rat skin without any significant permeation. Retention of DFV from nanoparticle in chitosan gel formulation (0.01%) was twofold higher than commercial cream, although it contained ten times less DFV. Nanoparticles in gel formulations produced significantly higher edema inhibition in rats compared with commercial cream in in-vivo studies. Skin blanching assay using a chromameter showed vasoconstriction similar to that of the commercial product. There were no barrier function changes upon application of nanoparticles. In-vitro and in-vivo results demonstrated that lecithin/chitosan nanoparticles in chitosan gel may be a promising carrier for dermal delivery of DFV in various skin disorders.Keywords: skin permeation, anti-inflammatory activity, skin blanching, TEWL
format	article
author	&Ouml;zcan İ Azizoğlu E Şenyiğit T &Ouml;zyazıcı M &Ouml;zer &Ouml;
author_facet	&Ouml;zcan İ Azizoğlu E Şenyiğit T &Ouml;zyazıcı M &Ouml;zer &Ouml;
author_sort	&Ouml;zcan İ
title	Enhanced dermal delivery of diflucortolone valerate using lecithin/chitosan nanoparticles: in-vitro and in-vivo evaluations
title_short	Enhanced dermal delivery of diflucortolone valerate using lecithin/chitosan nanoparticles: in-vitro and in-vivo evaluations
title_full	Enhanced dermal delivery of diflucortolone valerate using lecithin/chitosan nanoparticles: in-vitro and in-vivo evaluations
title_fullStr	Enhanced dermal delivery of diflucortolone valerate using lecithin/chitosan nanoparticles: in-vitro and in-vivo evaluations
title_full_unstemmed	Enhanced dermal delivery of diflucortolone valerate using lecithin/chitosan nanoparticles: in-vitro and in-vivo evaluations
title_sort	enhanced dermal delivery of diflucortolone valerate using lecithin/chitosan nanoparticles: in-vitro and in-vivo evaluations
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/4a1c4cdf070a489ea4ec5b4a07660cda
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Enhanced dermal delivery of diflucortolone valerate using lecithin/chitosan nanoparticles: in-vitro and in-vivo evaluations

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