Diethylstilbestrol administration inhibits theca cell androgen and granulosa cell estrogen production in immature rat ovary
Abstract Diethylstilbestrol (DES), a strong estrogenic compound, is well-known to affect the reproductive system. In this study, we investigated the effects of DES administration on gonadotropin levels and ovarian steroidogenesis in prepubertal rats. DES treatment acutely reduced serum LH levels, fo...
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2017
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oai:doaj.org-article:4a1dd609111b43a18d7f939cc20bbcdb2021-12-02T16:06:05ZDiethylstilbestrol administration inhibits theca cell androgen and granulosa cell estrogen production in immature rat ovary10.1038/s41598-017-08780-72045-2322https://doaj.org/article/4a1dd609111b43a18d7f939cc20bbcdb2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08780-7https://doaj.org/toc/2045-2322Abstract Diethylstilbestrol (DES), a strong estrogenic compound, is well-known to affect the reproductive system. In this study, we investigated the effects of DES administration on gonadotropin levels and ovarian steroidogenesis in prepubertal rats. DES treatment acutely reduced serum LH levels, followed by a reduction in the expression of various steroidogenesis-related genes in theca cells. Serum FSH levels were almost unaffected by DES-treatment, even though Cyp19a1 expression was markedly reduced. Serum progesterone, testosterone and estradiol levels were also declined at this time. LH levels recovered from 12 h after DES-treatment and gradually increased until 96 h with a reduction of ERα expression observed in the pituitary. Steroidogenesis-related genes were also up-regulated during this time, except for Cyp17a1 and Cyp19a1. Consistent with observed gene expression pattern, serum testosterone and estradiol concentrations were maintained at lower levels, even though progesterone levels recovered. DES-treatment induced the inducible nitric oxide synthase (iNOS) in granulosa cells, and a nitric oxide generator markedly repressed Cyp19a1 expression in cultured granulosa cells. These results indicate that DES inhibits thecal androgen production via suppression of pituitary LH secretion and ovarian Cyp17a1 expression. In addition, DES represses Cyp19a1 expression by inducing iNOS gene expression for continuous inhibition of estrogen production in granulosa cells.Yoshitaka ImamichiToshio SekiguchiTakeshi KitanoTakashi KajitaniReiko OkadaYoshihiko InaokaKaoru MiyamotoJunsuke UwadaSatoru TakahashiTakahiro NemotoAsuka ManoMd Rafiqul Islam KhanMd Tariqul IslamKoh-ichi YuhkiHitoshi KashiwagiFumitaka UshikubiNobuo SuzukiTakanobu TaniguchiTakashi YazawaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017) |
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Medicine R Science Q Yoshitaka Imamichi Toshio Sekiguchi Takeshi Kitano Takashi Kajitani Reiko Okada Yoshihiko Inaoka Kaoru Miyamoto Junsuke Uwada Satoru Takahashi Takahiro Nemoto Asuka Mano Md Rafiqul Islam Khan Md Tariqul Islam Koh-ichi Yuhki Hitoshi Kashiwagi Fumitaka Ushikubi Nobuo Suzuki Takanobu Taniguchi Takashi Yazawa Diethylstilbestrol administration inhibits theca cell androgen and granulosa cell estrogen production in immature rat ovary |
| description |
Abstract Diethylstilbestrol (DES), a strong estrogenic compound, is well-known to affect the reproductive system. In this study, we investigated the effects of DES administration on gonadotropin levels and ovarian steroidogenesis in prepubertal rats. DES treatment acutely reduced serum LH levels, followed by a reduction in the expression of various steroidogenesis-related genes in theca cells. Serum FSH levels were almost unaffected by DES-treatment, even though Cyp19a1 expression was markedly reduced. Serum progesterone, testosterone and estradiol levels were also declined at this time. LH levels recovered from 12 h after DES-treatment and gradually increased until 96 h with a reduction of ERα expression observed in the pituitary. Steroidogenesis-related genes were also up-regulated during this time, except for Cyp17a1 and Cyp19a1. Consistent with observed gene expression pattern, serum testosterone and estradiol concentrations were maintained at lower levels, even though progesterone levels recovered. DES-treatment induced the inducible nitric oxide synthase (iNOS) in granulosa cells, and a nitric oxide generator markedly repressed Cyp19a1 expression in cultured granulosa cells. These results indicate that DES inhibits thecal androgen production via suppression of pituitary LH secretion and ovarian Cyp17a1 expression. In addition, DES represses Cyp19a1 expression by inducing iNOS gene expression for continuous inhibition of estrogen production in granulosa cells. |
| format |
article |
| author |
Yoshitaka Imamichi Toshio Sekiguchi Takeshi Kitano Takashi Kajitani Reiko Okada Yoshihiko Inaoka Kaoru Miyamoto Junsuke Uwada Satoru Takahashi Takahiro Nemoto Asuka Mano Md Rafiqul Islam Khan Md Tariqul Islam Koh-ichi Yuhki Hitoshi Kashiwagi Fumitaka Ushikubi Nobuo Suzuki Takanobu Taniguchi Takashi Yazawa |
| author_facet |
Yoshitaka Imamichi Toshio Sekiguchi Takeshi Kitano Takashi Kajitani Reiko Okada Yoshihiko Inaoka Kaoru Miyamoto Junsuke Uwada Satoru Takahashi Takahiro Nemoto Asuka Mano Md Rafiqul Islam Khan Md Tariqul Islam Koh-ichi Yuhki Hitoshi Kashiwagi Fumitaka Ushikubi Nobuo Suzuki Takanobu Taniguchi Takashi Yazawa |
| author_sort |
Yoshitaka Imamichi |
| title |
Diethylstilbestrol administration inhibits theca cell androgen and granulosa cell estrogen production in immature rat ovary |
| title_short |
Diethylstilbestrol administration inhibits theca cell androgen and granulosa cell estrogen production in immature rat ovary |
| title_full |
Diethylstilbestrol administration inhibits theca cell androgen and granulosa cell estrogen production in immature rat ovary |
| title_fullStr |
Diethylstilbestrol administration inhibits theca cell androgen and granulosa cell estrogen production in immature rat ovary |
| title_full_unstemmed |
Diethylstilbestrol administration inhibits theca cell androgen and granulosa cell estrogen production in immature rat ovary |
| title_sort |
diethylstilbestrol administration inhibits theca cell androgen and granulosa cell estrogen production in immature rat ovary |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/4a1dd609111b43a18d7f939cc20bbcdb |
| work_keys_str_mv |
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| _version_ |
1718385086269751296 |