Integration of metabolomics and transcriptomics indicates changes in MRSA exposed to terpinen-4-ol
Abstract Background This study investigated the effects of terpinen-4-ol on methicillin-resistant Staphylococcus aureus (MRSA) and its biofilm, and the possible mechanisms governing this effect. Results We observed that terpinen-4-ol has good antibacterial activity and inhibits the formation of MRSA...
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2021
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oai:doaj.org-article:4a1f31f9dfa44a1bb4693aac33e87e6e2021-11-07T12:09:05ZIntegration of metabolomics and transcriptomics indicates changes in MRSA exposed to terpinen-4-ol10.1186/s12866-021-02348-21471-2180https://doaj.org/article/4a1f31f9dfa44a1bb4693aac33e87e6e2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12866-021-02348-2https://doaj.org/toc/1471-2180Abstract Background This study investigated the effects of terpinen-4-ol on methicillin-resistant Staphylococcus aureus (MRSA) and its biofilm, and the possible mechanisms governing this effect. Results We observed that terpinen-4-ol has good antibacterial activity and inhibits the formation of MRSA biofilm. The MIC and MBC values for terpinen-4-ol against S. aureus were 0.08% ~ 0.32%. And terpinen-4-ol at 0.32% could kill all bacteria and clear all biofilms. Untargeted metabolomic and transcriptomic analyses showed that terpinen-4-ol strongly inhibited DNA and RNA biosynthesis in MRSA at 2 h after treatment by affecting genes and metabolites related to purine and pyrimidine metabolic pathways. Some differential genes which play important roles in DNA synthesis and the production of eDNA from biofilm exposed to terpinen-4-ol was also significantly decreased compared with that of the control. Conclusions Terpinen-4-ol has good antibacterial activity and significantly inhibits the formation of MRSA biofilm by inhibiting purine and pyrimidine metabolism.Feng ChengYanan MoKeyuan ChenXiaofei ShangZhen YangBaocheng HaoRuofeng ShangJianping LiangYu LiuBMCarticleTerpinen-4-olMRSABiofilmMetabolomicsTranscriptomicsMicrobiologyQR1-502ENBMC Microbiology, Vol 21, Iss 1, Pp 1-12 (2021) |
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DOAJ |
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DOAJ |
| language |
EN |
| topic |
Terpinen-4-ol MRSA Biofilm Metabolomics Transcriptomics Microbiology QR1-502 |
| spellingShingle |
Terpinen-4-ol MRSA Biofilm Metabolomics Transcriptomics Microbiology QR1-502 Feng Cheng Yanan Mo Keyuan Chen Xiaofei Shang Zhen Yang Baocheng Hao Ruofeng Shang Jianping Liang Yu Liu Integration of metabolomics and transcriptomics indicates changes in MRSA exposed to terpinen-4-ol |
| description |
Abstract Background This study investigated the effects of terpinen-4-ol on methicillin-resistant Staphylococcus aureus (MRSA) and its biofilm, and the possible mechanisms governing this effect. Results We observed that terpinen-4-ol has good antibacterial activity and inhibits the formation of MRSA biofilm. The MIC and MBC values for terpinen-4-ol against S. aureus were 0.08% ~ 0.32%. And terpinen-4-ol at 0.32% could kill all bacteria and clear all biofilms. Untargeted metabolomic and transcriptomic analyses showed that terpinen-4-ol strongly inhibited DNA and RNA biosynthesis in MRSA at 2 h after treatment by affecting genes and metabolites related to purine and pyrimidine metabolic pathways. Some differential genes which play important roles in DNA synthesis and the production of eDNA from biofilm exposed to terpinen-4-ol was also significantly decreased compared with that of the control. Conclusions Terpinen-4-ol has good antibacterial activity and significantly inhibits the formation of MRSA biofilm by inhibiting purine and pyrimidine metabolism. |
| format |
article |
| author |
Feng Cheng Yanan Mo Keyuan Chen Xiaofei Shang Zhen Yang Baocheng Hao Ruofeng Shang Jianping Liang Yu Liu |
| author_facet |
Feng Cheng Yanan Mo Keyuan Chen Xiaofei Shang Zhen Yang Baocheng Hao Ruofeng Shang Jianping Liang Yu Liu |
| author_sort |
Feng Cheng |
| title |
Integration of metabolomics and transcriptomics indicates changes in MRSA exposed to terpinen-4-ol |
| title_short |
Integration of metabolomics and transcriptomics indicates changes in MRSA exposed to terpinen-4-ol |
| title_full |
Integration of metabolomics and transcriptomics indicates changes in MRSA exposed to terpinen-4-ol |
| title_fullStr |
Integration of metabolomics and transcriptomics indicates changes in MRSA exposed to terpinen-4-ol |
| title_full_unstemmed |
Integration of metabolomics and transcriptomics indicates changes in MRSA exposed to terpinen-4-ol |
| title_sort |
integration of metabolomics and transcriptomics indicates changes in mrsa exposed to terpinen-4-ol |
| publisher |
BMC |
| publishDate |
2021 |
| url |
https://doaj.org/article/4a1f31f9dfa44a1bb4693aac33e87e6e |
| work_keys_str_mv |
AT fengcheng integrationofmetabolomicsandtranscriptomicsindicateschangesinmrsaexposedtoterpinen4ol AT yananmo integrationofmetabolomicsandtranscriptomicsindicateschangesinmrsaexposedtoterpinen4ol AT keyuanchen integrationofmetabolomicsandtranscriptomicsindicateschangesinmrsaexposedtoterpinen4ol AT xiaofeishang integrationofmetabolomicsandtranscriptomicsindicateschangesinmrsaexposedtoterpinen4ol AT zhenyang integrationofmetabolomicsandtranscriptomicsindicateschangesinmrsaexposedtoterpinen4ol AT baochenghao integrationofmetabolomicsandtranscriptomicsindicateschangesinmrsaexposedtoterpinen4ol AT ruofengshang integrationofmetabolomicsandtranscriptomicsindicateschangesinmrsaexposedtoterpinen4ol AT jianpingliang integrationofmetabolomicsandtranscriptomicsindicateschangesinmrsaexposedtoterpinen4ol AT yuliu integrationofmetabolomicsandtranscriptomicsindicateschangesinmrsaexposedtoterpinen4ol |
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1718443544061935616 |