Measuring inter-protein pairwise interaction energies from a single native mass spectrum by double-mutant cycle analysis

Double mutant cycle (DMC) analyses can provide the interaction energies between amino acids at the interface of protein complexes. Here, the authors determine pairwise interaction energies using high-resolution native mass spectroscopy, offering a straightforward route for the DMC methodology.

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Autores principales:	Miri Sokolovski, Jelena Cveticanin, Déborah Hayoun, Ilia Korobko, Michal Sharon, Amnon Horovitz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/4a25bcb5b7644dde90dc2233ecee1ba5
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spelling	oai:doaj.org-article:4a25bcb5b7644dde90dc2233ecee1ba52021-12-02T10:48:28ZMeasuring inter-protein pairwise interaction energies from a single native mass spectrum by double-mutant cycle analysis10.1038/s41467-017-00285-12041-1723https://doaj.org/article/4a25bcb5b7644dde90dc2233ecee1ba52017-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-00285-1https://doaj.org/toc/2041-1723Double mutant cycle (DMC) analyses can provide the interaction energies between amino acids at the interface of protein complexes. Here, the authors determine pairwise interaction energies using high-resolution native mass spectroscopy, offering a straightforward route for the DMC methodology.Miri SokolovskiJelena CveticaninDéborah HayounIlia KorobkoMichal SharonAmnon HorovitzNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Miri Sokolovski Jelena Cveticanin Déborah Hayoun Ilia Korobko Michal Sharon Amnon Horovitz Measuring inter-protein pairwise interaction energies from a single native mass spectrum by double-mutant cycle analysis
description	Double mutant cycle (DMC) analyses can provide the interaction energies between amino acids at the interface of protein complexes. Here, the authors determine pairwise interaction energies using high-resolution native mass spectroscopy, offering a straightforward route for the DMC methodology.
format	article
author	Miri Sokolovski Jelena Cveticanin Déborah Hayoun Ilia Korobko Michal Sharon Amnon Horovitz
author_facet	Miri Sokolovski Jelena Cveticanin Déborah Hayoun Ilia Korobko Michal Sharon Amnon Horovitz
author_sort	Miri Sokolovski
title	Measuring inter-protein pairwise interaction energies from a single native mass spectrum by double-mutant cycle analysis
title_short	Measuring inter-protein pairwise interaction energies from a single native mass spectrum by double-mutant cycle analysis
title_full	Measuring inter-protein pairwise interaction energies from a single native mass spectrum by double-mutant cycle analysis
title_fullStr	Measuring inter-protein pairwise interaction energies from a single native mass spectrum by double-mutant cycle analysis
title_full_unstemmed	Measuring inter-protein pairwise interaction energies from a single native mass spectrum by double-mutant cycle analysis
title_sort	measuring inter-protein pairwise interaction energies from a single native mass spectrum by double-mutant cycle analysis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/4a25bcb5b7644dde90dc2233ecee1ba5
work_keys_str_mv	AT mirisokolovski measuringinterproteinpairwiseinteractionenergiesfromasinglenativemassspectrumbydoublemutantcycleanalysis AT jelenacveticanin measuringinterproteinpairwiseinteractionenergiesfromasinglenativemassspectrumbydoublemutantcycleanalysis AT deborahhayoun measuringinterproteinpairwiseinteractionenergiesfromasinglenativemassspectrumbydoublemutantcycleanalysis AT iliakorobko measuringinterproteinpairwiseinteractionenergiesfromasinglenativemassspectrumbydoublemutantcycleanalysis AT michalsharon measuringinterproteinpairwiseinteractionenergiesfromasinglenativemassspectrumbydoublemutantcycleanalysis AT amnonhorovitz measuringinterproteinpairwiseinteractionenergiesfromasinglenativemassspectrumbydoublemutantcycleanalysis
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Measuring inter-protein pairwise interaction energies from a single native mass spectrum by double-mutant cycle analysis

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