Identification of CRF66_BF, a New HIV-1 Circulating Recombinant Form of South American Origin

Circulating recombinant forms (CRFs) are important components of the HIV-1 pandemic. Among 110 reported in the literature, 17 are BF1 intersubtype recombinant, most of which are of South American origin. Among these, all 5 identified in the Southern Cone and neighboring countries, except Brazil, der...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Joan Bacqué, Elena Delgado, Sonia Benito, María Moreno-Lorenzo, Vanessa Montero, Horacio Gil, Mónica Sánchez, María Carmen Nieto-Toboso, Josefa Muñoz, Miren Z. Zubero-Sulibarria, Estíbaliz Ugalde, Elena García-Bodas, Javier E. Cañada, Jorge del Romero, Carmen Rodríguez, Iciar Rodríguez-Avial, Luis Elorduy-Otazua, José J. Portu, Juan García-Costa, Antonio Ocampo, Jorge J. Cabrera, Michael M. Thomson
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	HIV-1 circulating recombinant form molecular epidemiology phylogeny phylodynamics Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/4a2a56491b9b407fb77aba8447e8493a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4a2a56491b9b407fb77aba8447e8493a
record_format	dspace
spelling	oai:doaj.org-article:4a2a56491b9b407fb77aba8447e8493a2021-11-15T06:52:51ZIdentification of CRF66_BF, a New HIV-1 Circulating Recombinant Form of South American Origin1664-302X10.3389/fmicb.2021.774386https://doaj.org/article/4a2a56491b9b407fb77aba8447e8493a2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.774386/fullhttps://doaj.org/toc/1664-302XCirculating recombinant forms (CRFs) are important components of the HIV-1 pandemic. Among 110 reported in the literature, 17 are BF1 intersubtype recombinant, most of which are of South American origin. Among these, all 5 identified in the Southern Cone and neighboring countries, except Brazil, derive from a common recombinant ancestor related to CRF12_BF, which circulates widely in Argentina, as deduced from coincident breakpoints and clustering in phylogenetic trees. In a HIV-1 molecular epidemiological study in Spain, we identified a phylogenetic cluster of 20 samples from 3 separate regions which were of F1 subsubtype, related to the Brazilian strain, in protease-reverse transcriptase (Pr-RT) and of subtype B in integrase. Remarkably, 14 individuals from this cluster (designated BF9) were Paraguayans and only 4 were native Spaniards. HIV-1 transmission was predominantly heterosexual, except for a subcluster of 6 individuals, 5 of which were men who have sex with men. Ten additional database sequences, from Argentina (n = 4), Spain (n = 3), Paraguay (n = 1), Brazil (n = 1), and Italy (n = 1), branched within the BF9 cluster. To determine whether it represents a new CRF, near full-length genome (NFLG) sequences were obtained for 6 viruses from 3 Spanish regions. Bootscan analyses showed a coincident BF1 recombinant structure, with 5 breakpoints, located in p17gag, integrase, gp120, gp41-rev overlap, and nef, which was identical to that of two BF1 recombinant viruses from Paraguay previously sequenced in NFLGs. Interestingly, none of the breakpoints coincided with those of CRF12_BF. In a maximum likelihood phylogenetic tree, all 8 NFLG sequences grouped in a strongly supported clade segregating from previously identified CRFs and from the CRF12_BF “family” clade. These results allow us to identify a new HIV-1 CRF, designated CRF66_BF. Through a Bayesian coalescent analysis, the most recent common ancestor of CRF66_BF was estimated around 1984 in South America, either in Paraguay or Argentina. Among Pr-RT sequences obtained by us from HIV-1-infected Paraguayans living in Spain, 14 (20.9%) of 67 were of CRF66_BF, suggesting that CRF66_BF may be one of the major HIV-1 genetic forms circulating in Paraguay. CRF66_BF is the first reported non-Brazilian South American HIV-1 CRF_BF unrelated to CRF12_BF.Joan BacquéElena DelgadoSonia BenitoMaría Moreno-LorenzoVanessa MonteroHoracio GilMónica SánchezMaría Carmen Nieto-TobosoJosefa MuñozMiren Z. Zubero-SulibarriaEstíbaliz UgaldeElena García-BodasJavier E. CañadaJorge del RomeroCarmen RodríguezIciar Rodríguez-AvialLuis Elorduy-OtazuaJosé J. PortuJuan García-CostaAntonio OcampoJorge J. CabreraMichael M. ThomsonFrontiers Media S.A.articleHIV-1circulating recombinant formmolecular epidemiologyphylogenyphylodynamicsMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	HIV-1 circulating recombinant form molecular epidemiology phylogeny phylodynamics Microbiology QR1-502
spellingShingle	HIV-1 circulating recombinant form molecular epidemiology phylogeny phylodynamics Microbiology QR1-502 Joan Bacqué Elena Delgado Sonia Benito María Moreno-Lorenzo Vanessa Montero Horacio Gil Mónica Sánchez María Carmen Nieto-Toboso Josefa Muñoz Miren Z. Zubero-Sulibarria Estíbaliz Ugalde Elena García-Bodas Javier E. Cañada Jorge del Romero Carmen Rodríguez Iciar Rodríguez-Avial Luis Elorduy-Otazua José J. Portu Juan García-Costa Antonio Ocampo Jorge J. Cabrera Michael M. Thomson Identification of CRF66_BF, a New HIV-1 Circulating Recombinant Form of South American Origin
description	Circulating recombinant forms (CRFs) are important components of the HIV-1 pandemic. Among 110 reported in the literature, 17 are BF1 intersubtype recombinant, most of which are of South American origin. Among these, all 5 identified in the Southern Cone and neighboring countries, except Brazil, derive from a common recombinant ancestor related to CRF12_BF, which circulates widely in Argentina, as deduced from coincident breakpoints and clustering in phylogenetic trees. In a HIV-1 molecular epidemiological study in Spain, we identified a phylogenetic cluster of 20 samples from 3 separate regions which were of F1 subsubtype, related to the Brazilian strain, in protease-reverse transcriptase (Pr-RT) and of subtype B in integrase. Remarkably, 14 individuals from this cluster (designated BF9) were Paraguayans and only 4 were native Spaniards. HIV-1 transmission was predominantly heterosexual, except for a subcluster of 6 individuals, 5 of which were men who have sex with men. Ten additional database sequences, from Argentina (n = 4), Spain (n = 3), Paraguay (n = 1), Brazil (n = 1), and Italy (n = 1), branched within the BF9 cluster. To determine whether it represents a new CRF, near full-length genome (NFLG) sequences were obtained for 6 viruses from 3 Spanish regions. Bootscan analyses showed a coincident BF1 recombinant structure, with 5 breakpoints, located in p17gag, integrase, gp120, gp41-rev overlap, and nef, which was identical to that of two BF1 recombinant viruses from Paraguay previously sequenced in NFLGs. Interestingly, none of the breakpoints coincided with those of CRF12_BF. In a maximum likelihood phylogenetic tree, all 8 NFLG sequences grouped in a strongly supported clade segregating from previously identified CRFs and from the CRF12_BF “family” clade. These results allow us to identify a new HIV-1 CRF, designated CRF66_BF. Through a Bayesian coalescent analysis, the most recent common ancestor of CRF66_BF was estimated around 1984 in South America, either in Paraguay or Argentina. Among Pr-RT sequences obtained by us from HIV-1-infected Paraguayans living in Spain, 14 (20.9%) of 67 were of CRF66_BF, suggesting that CRF66_BF may be one of the major HIV-1 genetic forms circulating in Paraguay. CRF66_BF is the first reported non-Brazilian South American HIV-1 CRF_BF unrelated to CRF12_BF.
format	article
author	Joan Bacqué Elena Delgado Sonia Benito María Moreno-Lorenzo Vanessa Montero Horacio Gil Mónica Sánchez María Carmen Nieto-Toboso Josefa Muñoz Miren Z. Zubero-Sulibarria Estíbaliz Ugalde Elena García-Bodas Javier E. Cañada Jorge del Romero Carmen Rodríguez Iciar Rodríguez-Avial Luis Elorduy-Otazua José J. Portu Juan García-Costa Antonio Ocampo Jorge J. Cabrera Michael M. Thomson
author_facet	Joan Bacqué Elena Delgado Sonia Benito María Moreno-Lorenzo Vanessa Montero Horacio Gil Mónica Sánchez María Carmen Nieto-Toboso Josefa Muñoz Miren Z. Zubero-Sulibarria Estíbaliz Ugalde Elena García-Bodas Javier E. Cañada Jorge del Romero Carmen Rodríguez Iciar Rodríguez-Avial Luis Elorduy-Otazua José J. Portu Juan García-Costa Antonio Ocampo Jorge J. Cabrera Michael M. Thomson
author_sort	Joan Bacqué
title	Identification of CRF66_BF, a New HIV-1 Circulating Recombinant Form of South American Origin
title_short	Identification of CRF66_BF, a New HIV-1 Circulating Recombinant Form of South American Origin
title_full	Identification of CRF66_BF, a New HIV-1 Circulating Recombinant Form of South American Origin
title_fullStr	Identification of CRF66_BF, a New HIV-1 Circulating Recombinant Form of South American Origin
title_full_unstemmed	Identification of CRF66_BF, a New HIV-1 Circulating Recombinant Form of South American Origin
title_sort	identification of crf66_bf, a new hiv-1 circulating recombinant form of south american origin
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/4a2a56491b9b407fb77aba8447e8493a
work_keys_str_mv	AT joanbacque identificationofcrf66bfanewhiv1circulatingrecombinantformofsouthamericanorigin AT elenadelgado identificationofcrf66bfanewhiv1circulatingrecombinantformofsouthamericanorigin AT soniabenito identificationofcrf66bfanewhiv1circulatingrecombinantformofsouthamericanorigin AT mariamorenolorenzo identificationofcrf66bfanewhiv1circulatingrecombinantformofsouthamericanorigin AT vanessamontero identificationofcrf66bfanewhiv1circulatingrecombinantformofsouthamericanorigin AT horaciogil identificationofcrf66bfanewhiv1circulatingrecombinantformofsouthamericanorigin AT monicasanchez identificationofcrf66bfanewhiv1circulatingrecombinantformofsouthamericanorigin AT mariacarmennietotoboso identificationofcrf66bfanewhiv1circulatingrecombinantformofsouthamericanorigin AT josefamunoz identificationofcrf66bfanewhiv1circulatingrecombinantformofsouthamericanorigin AT mirenzzuberosulibarria identificationofcrf66bfanewhiv1circulatingrecombinantformofsouthamericanorigin AT estibalizugalde identificationofcrf66bfanewhiv1circulatingrecombinantformofsouthamericanorigin AT elenagarciabodas identificationofcrf66bfanewhiv1circulatingrecombinantformofsouthamericanorigin AT javierecanada identificationofcrf66bfanewhiv1circulatingrecombinantformofsouthamericanorigin AT jorgedelromero identificationofcrf66bfanewhiv1circulatingrecombinantformofsouthamericanorigin AT carmenrodriguez identificationofcrf66bfanewhiv1circulatingrecombinantformofsouthamericanorigin AT iciarrodriguezavial identificationofcrf66bfanewhiv1circulatingrecombinantformofsouthamericanorigin AT luiselorduyotazua identificationofcrf66bfanewhiv1circulatingrecombinantformofsouthamericanorigin AT josejportu identificationofcrf66bfanewhiv1circulatingrecombinantformofsouthamericanorigin AT juangarciacosta identificationofcrf66bfanewhiv1circulatingrecombinantformofsouthamericanorigin AT antonioocampo identificationofcrf66bfanewhiv1circulatingrecombinantformofsouthamericanorigin AT jorgejcabrera identificationofcrf66bfanewhiv1circulatingrecombinantformofsouthamericanorigin AT michaelmthomson identificationofcrf66bfanewhiv1circulatingrecombinantformofsouthamericanorigin
_version_	1718428550518800384

Identification of CRF66_BF, a New HIV-1 Circulating Recombinant Form of South American Origin

Ejemplares similares