Novel quantitative autophagy analysis by organelle flow cytometry after cell sonication.
Autophagy is a dynamic process of bulk degradation of cellular proteins and organelles in lysosomes. Current methods of autophagy measurement include microscopy-based counting of autophagic vacuoles (AVs) in cells. We have developed a novel method to quantitatively analyze individual AVs using flow...
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Autores principales: | Michael Degtyarev, Mike Reichelt, Kui Lin |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Public Library of Science (PLoS)
2014
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Materias: | |
Acceso en línea: | https://doaj.org/article/4a2b0d377fd54403b86f84c3184b5089 |
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