Novel quantitative autophagy analysis by organelle flow cytometry after cell sonication.

Autophagy is a dynamic process of bulk degradation of cellular proteins and organelles in lysosomes. Current methods of autophagy measurement include microscopy-based counting of autophagic vacuoles (AVs) in cells. We have developed a novel method to quantitatively analyze individual AVs using flow...

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Autores principales: Michael Degtyarev, Mike Reichelt, Kui Lin
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2014
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Acceso en línea:https://doaj.org/article/4a2b0d377fd54403b86f84c3184b5089
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