Preparation of Specific Nanobodies and Their Application in the Rapid Detection of Nodularin-R in Water Samples

Nanobodies have several advantages, including great stability, sensibility, and ease of production; therefore, they have become important tools in immunoassays for chemical contaminants. In this manuscript, nanobodies for the detection of the toxin Nodularin-r (NOD-R), a secondary metabolite of cyan...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jinyi Yang, Rui Si, Guangpei Wu, Yu Wang, Ruyu Fang, Fei Liu, Feng Wang, Hongtao Lei, Yudong Shen, Qi Zhang, Hong Wang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	nodularin nanobody detection ic-ELISA Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/4a2b7f48c083456eb522f5101aac880a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4a2b7f48c083456eb522f5101aac880a
record_format	dspace
spelling	oai:doaj.org-article:4a2b7f48c083456eb522f5101aac880a2021-11-25T17:35:20ZPreparation of Specific Nanobodies and Their Application in the Rapid Detection of Nodularin-R in Water Samples10.3390/foods101127582304-8158https://doaj.org/article/4a2b7f48c083456eb522f5101aac880a2021-11-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2758https://doaj.org/toc/2304-8158Nanobodies have several advantages, including great stability, sensibility, and ease of production; therefore, they have become important tools in immunoassays for chemical contaminants. In this manuscript, nanobodies for the detection of the toxin Nodularin-r (NOD-R), a secondary metabolite of cyanobacteria that could cause a safety risk for drinks and food for its strong hepatotoxicity, were for the first time selected from an immunized Bactrian camel VHH phage display library. Then, a sensitive indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for NOD-R, based on the nanobody N56 with great thermostability and organic solvent tolerance, was established under optimized conditions. The results showed that the limit of detection for NOD-R was 0.67 µg/L, and the average spike recovery rate was between 84.0 and 118.3%. Moreover, the ic-ELISA method was validated with spiked water sample and confirmed by UPLC–MS/MS, which indicated that the ic-ELISA established in this work is a reproducible detection assay for nodularin residues in water samples.Jinyi YangRui SiGuangpei WuYu WangRuyu FangFei LiuFeng WangHongtao LeiYudong ShenQi ZhangHong WangMDPI AGarticlenodularinnanobodydetectionic-ELISAChemical technologyTP1-1185ENFoods, Vol 10, Iss 2758, p 2758 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	nodularin nanobody detection ic-ELISA Chemical technology TP1-1185
spellingShingle	nodularin nanobody detection ic-ELISA Chemical technology TP1-1185 Jinyi Yang Rui Si Guangpei Wu Yu Wang Ruyu Fang Fei Liu Feng Wang Hongtao Lei Yudong Shen Qi Zhang Hong Wang Preparation of Specific Nanobodies and Their Application in the Rapid Detection of Nodularin-R in Water Samples
description	Nanobodies have several advantages, including great stability, sensibility, and ease of production; therefore, they have become important tools in immunoassays for chemical contaminants. In this manuscript, nanobodies for the detection of the toxin Nodularin-r (NOD-R), a secondary metabolite of cyanobacteria that could cause a safety risk for drinks and food for its strong hepatotoxicity, were for the first time selected from an immunized Bactrian camel VHH phage display library. Then, a sensitive indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for NOD-R, based on the nanobody N56 with great thermostability and organic solvent tolerance, was established under optimized conditions. The results showed that the limit of detection for NOD-R was 0.67 µg/L, and the average spike recovery rate was between 84.0 and 118.3%. Moreover, the ic-ELISA method was validated with spiked water sample and confirmed by UPLC–MS/MS, which indicated that the ic-ELISA established in this work is a reproducible detection assay for nodularin residues in water samples.
format	article
author	Jinyi Yang Rui Si Guangpei Wu Yu Wang Ruyu Fang Fei Liu Feng Wang Hongtao Lei Yudong Shen Qi Zhang Hong Wang
author_facet	Jinyi Yang Rui Si Guangpei Wu Yu Wang Ruyu Fang Fei Liu Feng Wang Hongtao Lei Yudong Shen Qi Zhang Hong Wang
author_sort	Jinyi Yang
title	Preparation of Specific Nanobodies and Their Application in the Rapid Detection of Nodularin-R in Water Samples
title_short	Preparation of Specific Nanobodies and Their Application in the Rapid Detection of Nodularin-R in Water Samples
title_full	Preparation of Specific Nanobodies and Their Application in the Rapid Detection of Nodularin-R in Water Samples
title_fullStr	Preparation of Specific Nanobodies and Their Application in the Rapid Detection of Nodularin-R in Water Samples
title_full_unstemmed	Preparation of Specific Nanobodies and Their Application in the Rapid Detection of Nodularin-R in Water Samples
title_sort	preparation of specific nanobodies and their application in the rapid detection of nodularin-r in water samples
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4a2b7f48c083456eb522f5101aac880a
work_keys_str_mv	AT jinyiyang preparationofspecificnanobodiesandtheirapplicationintherapiddetectionofnodularinrinwatersamples AT ruisi preparationofspecificnanobodiesandtheirapplicationintherapiddetectionofnodularinrinwatersamples AT guangpeiwu preparationofspecificnanobodiesandtheirapplicationintherapiddetectionofnodularinrinwatersamples AT yuwang preparationofspecificnanobodiesandtheirapplicationintherapiddetectionofnodularinrinwatersamples AT ruyufang preparationofspecificnanobodiesandtheirapplicationintherapiddetectionofnodularinrinwatersamples AT feiliu preparationofspecificnanobodiesandtheirapplicationintherapiddetectionofnodularinrinwatersamples AT fengwang preparationofspecificnanobodiesandtheirapplicationintherapiddetectionofnodularinrinwatersamples AT hongtaolei preparationofspecificnanobodiesandtheirapplicationintherapiddetectionofnodularinrinwatersamples AT yudongshen preparationofspecificnanobodiesandtheirapplicationintherapiddetectionofnodularinrinwatersamples AT qizhang preparationofspecificnanobodiesandtheirapplicationintherapiddetectionofnodularinrinwatersamples AT hongwang preparationofspecificnanobodiesandtheirapplicationintherapiddetectionofnodularinrinwatersamples
_version_	1718412162631729152

Preparation of Specific Nanobodies and Their Application in the Rapid Detection of Nodularin-R in Water Samples

Ejemplares similares