Cell-free DNA promotes malignant transformation in non-tumor cells

Abstract Cell-free DNA is present in different biological fluids and when released by tumor cells may contribute to pro-tumor events such as malignant transformation of cells adjacent to the tumor and metastasis. Thus, this study analyzed the effect of tumor cell-free DNA, isolated from the blood of...

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Autores principales:	Aline Gomes de Souza, Victor Alexandre F. Bastos, Patricia Tieme Fujimura, Izabella Cristina C. Ferreira, Letícia Ferro Leal, Luciane Sussuchi da Silva, Ana Carolina Laus, Rui Manuel Reis, Mario Machado Martins, Paula Souza Santos, Natássia C. Resende Corrêa, Karina Marangoni, Carolina Hassibe Thomé, Leandro Machado Colli, Luiz Ricardo Goulart, Vivian Alonso Goulart
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4a2f8801e2a2482b9d2e08e45f5001d1
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spelling	oai:doaj.org-article:4a2f8801e2a2482b9d2e08e45f5001d12021-12-02T15:11:52ZCell-free DNA promotes malignant transformation in non-tumor cells10.1038/s41598-020-78766-52045-2322https://doaj.org/article/4a2f8801e2a2482b9d2e08e45f5001d12020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78766-5https://doaj.org/toc/2045-2322Abstract Cell-free DNA is present in different biological fluids and when released by tumor cells may contribute to pro-tumor events such as malignant transformation of cells adjacent to the tumor and metastasis. Thus, this study analyzed the effect of tumor cell-free DNA, isolated from the blood of prostate cancer patients, on non-tumor prostate cell lines (RWPE-1 and PNT-2). To achieve this, we performed cell-free DNA quantification and characterization assays, evaluation of gene and miRNA expression profiling focused on cancer progression and EMT, and metabolomics by mass spectrometry and cellular migration. The results showed that tumor-free cell DNA was able to alter the gene expression of MMP9 and CD44, alter the expression profile of nine miRNAs, and increased the tryptophan consumption and cell migration rates in non-tumor cells. Therefore, tumor cell-free DNA was capable of altering the receptor cell phenotype, triggering events related to malignant transformation in these cells, and can thus be considered a potential target for cancer diagnosis and therapy.Aline Gomes de SouzaVictor Alexandre F. BastosPatricia Tieme FujimuraIzabella Cristina C. FerreiraLetícia Ferro LealLuciane Sussuchi da SilvaAna Carolina LausRui Manuel ReisMario Machado MartinsPaula Souza SantosNatássia C. Resende CorrêaKarina MarangoniCarolina Hassibe ThoméLeandro Machado ColliLuiz Ricardo GoulartVivian Alonso GoulartNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aline Gomes de Souza Victor Alexandre F. Bastos Patricia Tieme Fujimura Izabella Cristina C. Ferreira Letícia Ferro Leal Luciane Sussuchi da Silva Ana Carolina Laus Rui Manuel Reis Mario Machado Martins Paula Souza Santos Natássia C. Resende Corrêa Karina Marangoni Carolina Hassibe Thomé Leandro Machado Colli Luiz Ricardo Goulart Vivian Alonso Goulart Cell-free DNA promotes malignant transformation in non-tumor cells
description	Abstract Cell-free DNA is present in different biological fluids and when released by tumor cells may contribute to pro-tumor events such as malignant transformation of cells adjacent to the tumor and metastasis. Thus, this study analyzed the effect of tumor cell-free DNA, isolated from the blood of prostate cancer patients, on non-tumor prostate cell lines (RWPE-1 and PNT-2). To achieve this, we performed cell-free DNA quantification and characterization assays, evaluation of gene and miRNA expression profiling focused on cancer progression and EMT, and metabolomics by mass spectrometry and cellular migration. The results showed that tumor-free cell DNA was able to alter the gene expression of MMP9 and CD44, alter the expression profile of nine miRNAs, and increased the tryptophan consumption and cell migration rates in non-tumor cells. Therefore, tumor cell-free DNA was capable of altering the receptor cell phenotype, triggering events related to malignant transformation in these cells, and can thus be considered a potential target for cancer diagnosis and therapy.
format	article
author	Aline Gomes de Souza Victor Alexandre F. Bastos Patricia Tieme Fujimura Izabella Cristina C. Ferreira Letícia Ferro Leal Luciane Sussuchi da Silva Ana Carolina Laus Rui Manuel Reis Mario Machado Martins Paula Souza Santos Natássia C. Resende Corrêa Karina Marangoni Carolina Hassibe Thomé Leandro Machado Colli Luiz Ricardo Goulart Vivian Alonso Goulart
author_facet	Aline Gomes de Souza Victor Alexandre F. Bastos Patricia Tieme Fujimura Izabella Cristina C. Ferreira Letícia Ferro Leal Luciane Sussuchi da Silva Ana Carolina Laus Rui Manuel Reis Mario Machado Martins Paula Souza Santos Natássia C. Resende Corrêa Karina Marangoni Carolina Hassibe Thomé Leandro Machado Colli Luiz Ricardo Goulart Vivian Alonso Goulart
author_sort	Aline Gomes de Souza
title	Cell-free DNA promotes malignant transformation in non-tumor cells
title_short	Cell-free DNA promotes malignant transformation in non-tumor cells
title_full	Cell-free DNA promotes malignant transformation in non-tumor cells
title_fullStr	Cell-free DNA promotes malignant transformation in non-tumor cells
title_full_unstemmed	Cell-free DNA promotes malignant transformation in non-tumor cells
title_sort	cell-free dna promotes malignant transformation in non-tumor cells
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/4a2f8801e2a2482b9d2e08e45f5001d1
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Cell-free DNA promotes malignant transformation in non-tumor cells

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