Expression Dynamics of the O-Glycosylated Proteins Recognized by Amaranthus leucocarpus Lectin in T Lymphocytes and Its Relationship With Moesin as an Alternative Mechanism of Cell Activation

T lymphocyte activation begins with antigen/MHC recognition by the TCR/CD3 complex followed by a costimulatory signal provided by CD28. The search for novel costimulatory molecules has been extensive due to their potential use as immunotherapeutic targets. Although some molecules have been identifie...

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Autores principales:	Wilton Gómez-Henao, Rafael Saavedra, Francisco Raúl Chávez-Sánchez, Ricardo Lascurain, Edgar Zenteno, Eda Patricia Tenorio
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	O-glycosylation Amaranthus leucocarpus lectin moesin T lymphocytes costimulatory molecules Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/4a30759d829244a6a99b0898ece3c9f5
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spelling	oai:doaj.org-article:4a30759d829244a6a99b0898ece3c9f52021-12-01T18:25:59ZExpression Dynamics of the O-Glycosylated Proteins Recognized by Amaranthus leucocarpus Lectin in T Lymphocytes and Its Relationship With Moesin as an Alternative Mechanism of Cell Activation1664-322410.3389/fimmu.2021.788880https://doaj.org/article/4a30759d829244a6a99b0898ece3c9f52021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.788880/fullhttps://doaj.org/toc/1664-3224T lymphocyte activation begins with antigen/MHC recognition by the TCR/CD3 complex followed by a costimulatory signal provided by CD28. The search for novel costimulatory molecules has been extensive due to their potential use as immunotherapeutic targets. Although some molecules have been identified, they are unable to provide sustainable signaling to allow for proper T cell activation and proliferation. It has been shown that the Amaranthus leucocarpus lectin (ALL) can be used as an in vitro costimulator of CD4+ lymphocytes in the presence of anti-CD3 mAb; this lectin specifically recognizes O-glycans of the Galβ1-3GalNAc-O-Ser/Thr type, including a 70-kDa moesin-like protein that has been suggested as the costimulatory molecule. However, the identity of this molecule has not been confirmed and such costimulation has not been analyzed in CD8+ lymphocytes. We show herein that the expression kinetics of the glycoproteins recognized by ALL (gpALL) is different in CD4+ and CD8+ T cells, unlike moesin expression. Results from IP experiments demonstrate that the previously described 70-kDa moesin-like protein is an O-glycosylated form of moesin (O-moesin) and that in vitro stimulation with anti-CD3 and anti-moesin mAb induces expression of the activation molecules CD69 and CD25, proliferation and IL-2 production as efficiently as cells costimulated with ALL or anti-CD28. Overall, our results demonstrate that O-moesin is expressed in CD4+ and CD8+ T lymphocytes and that moesin provides a new costimulatory activation signal in both T cell subsets.Wilton Gómez-HenaoRafael SaavedraFrancisco Raúl Chávez-SánchezRicardo LascurainEdgar ZentenoEda Patricia TenorioFrontiers Media S.A.articleO-glycosylationAmaranthus leucocarpus lectinmoesinT lymphocytescostimulatory moleculesImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	O-glycosylation Amaranthus leucocarpus lectin moesin T lymphocytes costimulatory molecules Immunologic diseases. Allergy RC581-607
spellingShingle	O-glycosylation Amaranthus leucocarpus lectin moesin T lymphocytes costimulatory molecules Immunologic diseases. Allergy RC581-607 Wilton Gómez-Henao Rafael Saavedra Francisco Raúl Chávez-Sánchez Ricardo Lascurain Edgar Zenteno Eda Patricia Tenorio Expression Dynamics of the O-Glycosylated Proteins Recognized by Amaranthus leucocarpus Lectin in T Lymphocytes and Its Relationship With Moesin as an Alternative Mechanism of Cell Activation
description	T lymphocyte activation begins with antigen/MHC recognition by the TCR/CD3 complex followed by a costimulatory signal provided by CD28. The search for novel costimulatory molecules has been extensive due to their potential use as immunotherapeutic targets. Although some molecules have been identified, they are unable to provide sustainable signaling to allow for proper T cell activation and proliferation. It has been shown that the Amaranthus leucocarpus lectin (ALL) can be used as an in vitro costimulator of CD4+ lymphocytes in the presence of anti-CD3 mAb; this lectin specifically recognizes O-glycans of the Galβ1-3GalNAc-O-Ser/Thr type, including a 70-kDa moesin-like protein that has been suggested as the costimulatory molecule. However, the identity of this molecule has not been confirmed and such costimulation has not been analyzed in CD8+ lymphocytes. We show herein that the expression kinetics of the glycoproteins recognized by ALL (gpALL) is different in CD4+ and CD8+ T cells, unlike moesin expression. Results from IP experiments demonstrate that the previously described 70-kDa moesin-like protein is an O-glycosylated form of moesin (O-moesin) and that in vitro stimulation with anti-CD3 and anti-moesin mAb induces expression of the activation molecules CD69 and CD25, proliferation and IL-2 production as efficiently as cells costimulated with ALL or anti-CD28. Overall, our results demonstrate that O-moesin is expressed in CD4+ and CD8+ T lymphocytes and that moesin provides a new costimulatory activation signal in both T cell subsets.
format	article
author	Wilton Gómez-Henao Rafael Saavedra Francisco Raúl Chávez-Sánchez Ricardo Lascurain Edgar Zenteno Eda Patricia Tenorio
author_facet	Wilton Gómez-Henao Rafael Saavedra Francisco Raúl Chávez-Sánchez Ricardo Lascurain Edgar Zenteno Eda Patricia Tenorio
author_sort	Wilton Gómez-Henao
title	Expression Dynamics of the O-Glycosylated Proteins Recognized by Amaranthus leucocarpus Lectin in T Lymphocytes and Its Relationship With Moesin as an Alternative Mechanism of Cell Activation
title_short	Expression Dynamics of the O-Glycosylated Proteins Recognized by Amaranthus leucocarpus Lectin in T Lymphocytes and Its Relationship With Moesin as an Alternative Mechanism of Cell Activation
title_full	Expression Dynamics of the O-Glycosylated Proteins Recognized by Amaranthus leucocarpus Lectin in T Lymphocytes and Its Relationship With Moesin as an Alternative Mechanism of Cell Activation
title_fullStr	Expression Dynamics of the O-Glycosylated Proteins Recognized by Amaranthus leucocarpus Lectin in T Lymphocytes and Its Relationship With Moesin as an Alternative Mechanism of Cell Activation
title_full_unstemmed	Expression Dynamics of the O-Glycosylated Proteins Recognized by Amaranthus leucocarpus Lectin in T Lymphocytes and Its Relationship With Moesin as an Alternative Mechanism of Cell Activation
title_sort	expression dynamics of the o-glycosylated proteins recognized by amaranthus leucocarpus lectin in t lymphocytes and its relationship with moesin as an alternative mechanism of cell activation
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/4a30759d829244a6a99b0898ece3c9f5
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Expression Dynamics of the O-Glycosylated Proteins Recognized by Amaranthus leucocarpus Lectin in T Lymphocytes and Its Relationship With Moesin as an Alternative Mechanism of Cell Activation

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