Human primary endothelial label-free biochip assay reveals unpredicted functions of plasma serine proteases

Abstract Tissue-on-a-chip technologies are more and more important in the investigation of cellular function and in the development of novel drugs by allowing the direct screening of substances on human cells. Constituting the inner lining of vessel walls, endothelial cells are the key players in va...

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Autores principales:	Márta Lídia Debreczeni, Inna Szekacs, Boglarka Kovacs, Andras Saftics, Sándor Kurunczi, Péter Gál, József Dobó, László Cervenak, Robert Horvath
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4a34a3f64d1944fc8cd62fa49dcdda56
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spelling	oai:doaj.org-article:4a34a3f64d1944fc8cd62fa49dcdda562021-12-02T10:59:52ZHuman primary endothelial label-free biochip assay reveals unpredicted functions of plasma serine proteases10.1038/s41598-020-60158-42045-2322https://doaj.org/article/4a34a3f64d1944fc8cd62fa49dcdda562020-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-60158-4https://doaj.org/toc/2045-2322Abstract Tissue-on-a-chip technologies are more and more important in the investigation of cellular function and in the development of novel drugs by allowing the direct screening of substances on human cells. Constituting the inner lining of vessel walls, endothelial cells are the key players in various physiological processes, moreover, they are the first to be exposed to most drugs currently used. However, to date, there is still no appropriate technology for the label-free, real-time and high-throughput monitoring of endothelial function. To this end, we developed an optical biosensor-based endothelial label-free biochip (EnLaB) assay that meets all the above requirements. Using our EnLaB platform, we screened a set of plasma serine proteases as possible endothelial cell activators, and first identified the endothelial cell activating function of three important serine proteases – namely kallikrein, C1r and mannan-binding lectin-associated serine-protease 2 (MASP-2) – and verified these results in well-established functional assays. EnLaB proved to be an effective tool for revealing novel cellular mechanisms as well as for the high-throughput screening of various compounds on endothelial cells.Márta Lídia DebreczeniInna SzekacsBoglarka KovacsAndras SafticsSándor KuruncziPéter GálJózsef DobóLászló CervenakRobert HorvathNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-14 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Márta Lídia Debreczeni Inna Szekacs Boglarka Kovacs Andras Saftics Sándor Kurunczi Péter Gál József Dobó László Cervenak Robert Horvath Human primary endothelial label-free biochip assay reveals unpredicted functions of plasma serine proteases
description	Abstract Tissue-on-a-chip technologies are more and more important in the investigation of cellular function and in the development of novel drugs by allowing the direct screening of substances on human cells. Constituting the inner lining of vessel walls, endothelial cells are the key players in various physiological processes, moreover, they are the first to be exposed to most drugs currently used. However, to date, there is still no appropriate technology for the label-free, real-time and high-throughput monitoring of endothelial function. To this end, we developed an optical biosensor-based endothelial label-free biochip (EnLaB) assay that meets all the above requirements. Using our EnLaB platform, we screened a set of plasma serine proteases as possible endothelial cell activators, and first identified the endothelial cell activating function of three important serine proteases – namely kallikrein, C1r and mannan-binding lectin-associated serine-protease 2 (MASP-2) – and verified these results in well-established functional assays. EnLaB proved to be an effective tool for revealing novel cellular mechanisms as well as for the high-throughput screening of various compounds on endothelial cells.
format	article
author	Márta Lídia Debreczeni Inna Szekacs Boglarka Kovacs Andras Saftics Sándor Kurunczi Péter Gál József Dobó László Cervenak Robert Horvath
author_facet	Márta Lídia Debreczeni Inna Szekacs Boglarka Kovacs Andras Saftics Sándor Kurunczi Péter Gál József Dobó László Cervenak Robert Horvath
author_sort	Márta Lídia Debreczeni
title	Human primary endothelial label-free biochip assay reveals unpredicted functions of plasma serine proteases
title_short	Human primary endothelial label-free biochip assay reveals unpredicted functions of plasma serine proteases
title_full	Human primary endothelial label-free biochip assay reveals unpredicted functions of plasma serine proteases
title_fullStr	Human primary endothelial label-free biochip assay reveals unpredicted functions of plasma serine proteases
title_full_unstemmed	Human primary endothelial label-free biochip assay reveals unpredicted functions of plasma serine proteases
title_sort	human primary endothelial label-free biochip assay reveals unpredicted functions of plasma serine proteases
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/4a34a3f64d1944fc8cd62fa49dcdda56
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Human primary endothelial label-free biochip assay reveals unpredicted functions of plasma serine proteases

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