The mannose receptor (CD206) identifies a population of colonic macrophages in health and inflammatory bowel disease

Abstract To understand the contribution of mononuclear phagocytes (MNP), which include monocyte-derived intestinal macrophages, to the pathogenesis of inflammatory bowel disease (IBD), it is necessary to identify functionally-different MNP populations. We aimed to characterise intestinal macrophage...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Pamela B. Wright, Elizabeth McDonald, Alberto Bravo-Blas, Hannah M. Baer, Anna Heawood, Calum C. Bain, Allan M. Mowat, Slater L. Clay, Elaine V. Robertson, Fraser Morton, Jagtar Singh Nijjar, Umer Z. Ijaz, Simon W. F. Milling, Daniel R. Gaya
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4a39c7bad69f4132860514691588ed0f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4a39c7bad69f4132860514691588ed0f
record_format	dspace
spelling	oai:doaj.org-article:4a39c7bad69f4132860514691588ed0f2021-12-02T16:57:08ZThe mannose receptor (CD206) identifies a population of colonic macrophages in health and inflammatory bowel disease10.1038/s41598-021-98611-72045-2322https://doaj.org/article/4a39c7bad69f4132860514691588ed0f2021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98611-7https://doaj.org/toc/2045-2322Abstract To understand the contribution of mononuclear phagocytes (MNP), which include monocyte-derived intestinal macrophages, to the pathogenesis of inflammatory bowel disease (IBD), it is necessary to identify functionally-different MNP populations. We aimed to characterise intestinal macrophage populations in patients with IBD. We developed 12-parameter flow cytometry protocols to identify and human intestinal MNPs. We used these protocols to purify and characterize colonic macrophages from colonic tissue from patients with Crohn’s disease (CD), ulcerative colitis (UC), or non-inflamed controls, in a cross-sectional study. We identify macrophage populations (CD45+CD64+ HLA-DR+) and describe two distinct subsets, differentiated by their expression of the mannose receptor, CD206. CD206+ macrophages expressed markers consistent with a mature phenotype: high levels of CD68 and CD163, higher transcription of IL-10 and lower expression of TREM1. CD206− macrophages appear to be less mature, with features more similar to their monocytic precursors. We identified and purified macrophage populations from human colon. These appear to be derived from a monocytic precursor with high CCR2 and low CD206 expression. As these cells mature, they acquire expression of IL-10, CD206, CD63, and CD168. Targeting the newly recruited monocyte-derived cells may represent a fruitful avenue to ameliorate chronic inflammation in IBD.Pamela B. WrightElizabeth McDonaldAlberto Bravo-BlasHannah M. BaerAnna HeawoodCalum C. BainAllan M. MowatSlater L. ClayElaine V. RobertsonFraser MortonJagtar Singh NijjarUmer Z. IjazSimon W. F. MillingDaniel R. GayaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pamela B. Wright Elizabeth McDonald Alberto Bravo-Blas Hannah M. Baer Anna Heawood Calum C. Bain Allan M. Mowat Slater L. Clay Elaine V. Robertson Fraser Morton Jagtar Singh Nijjar Umer Z. Ijaz Simon W. F. Milling Daniel R. Gaya The mannose receptor (CD206) identifies a population of colonic macrophages in health and inflammatory bowel disease
description	Abstract To understand the contribution of mononuclear phagocytes (MNP), which include monocyte-derived intestinal macrophages, to the pathogenesis of inflammatory bowel disease (IBD), it is necessary to identify functionally-different MNP populations. We aimed to characterise intestinal macrophage populations in patients with IBD. We developed 12-parameter flow cytometry protocols to identify and human intestinal MNPs. We used these protocols to purify and characterize colonic macrophages from colonic tissue from patients with Crohn’s disease (CD), ulcerative colitis (UC), or non-inflamed controls, in a cross-sectional study. We identify macrophage populations (CD45+CD64+ HLA-DR+) and describe two distinct subsets, differentiated by their expression of the mannose receptor, CD206. CD206+ macrophages expressed markers consistent with a mature phenotype: high levels of CD68 and CD163, higher transcription of IL-10 and lower expression of TREM1. CD206− macrophages appear to be less mature, with features more similar to their monocytic precursors. We identified and purified macrophage populations from human colon. These appear to be derived from a monocytic precursor with high CCR2 and low CD206 expression. As these cells mature, they acquire expression of IL-10, CD206, CD63, and CD168. Targeting the newly recruited monocyte-derived cells may represent a fruitful avenue to ameliorate chronic inflammation in IBD.
format	article
author	Pamela B. Wright Elizabeth McDonald Alberto Bravo-Blas Hannah M. Baer Anna Heawood Calum C. Bain Allan M. Mowat Slater L. Clay Elaine V. Robertson Fraser Morton Jagtar Singh Nijjar Umer Z. Ijaz Simon W. F. Milling Daniel R. Gaya
author_facet	Pamela B. Wright Elizabeth McDonald Alberto Bravo-Blas Hannah M. Baer Anna Heawood Calum C. Bain Allan M. Mowat Slater L. Clay Elaine V. Robertson Fraser Morton Jagtar Singh Nijjar Umer Z. Ijaz Simon W. F. Milling Daniel R. Gaya
author_sort	Pamela B. Wright
title	The mannose receptor (CD206) identifies a population of colonic macrophages in health and inflammatory bowel disease
title_short	The mannose receptor (CD206) identifies a population of colonic macrophages in health and inflammatory bowel disease
title_full	The mannose receptor (CD206) identifies a population of colonic macrophages in health and inflammatory bowel disease
title_fullStr	The mannose receptor (CD206) identifies a population of colonic macrophages in health and inflammatory bowel disease
title_full_unstemmed	The mannose receptor (CD206) identifies a population of colonic macrophages in health and inflammatory bowel disease
title_sort	mannose receptor (cd206) identifies a population of colonic macrophages in health and inflammatory bowel disease
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4a39c7bad69f4132860514691588ed0f
work_keys_str_mv	AT pamelabwright themannosereceptorcd206identifiesapopulationofcolonicmacrophagesinhealthandinflammatoryboweldisease AT elizabethmcdonald themannosereceptorcd206identifiesapopulationofcolonicmacrophagesinhealthandinflammatoryboweldisease AT albertobravoblas themannosereceptorcd206identifiesapopulationofcolonicmacrophagesinhealthandinflammatoryboweldisease AT hannahmbaer themannosereceptorcd206identifiesapopulationofcolonicmacrophagesinhealthandinflammatoryboweldisease AT annaheawood themannosereceptorcd206identifiesapopulationofcolonicmacrophagesinhealthandinflammatoryboweldisease AT calumcbain themannosereceptorcd206identifiesapopulationofcolonicmacrophagesinhealthandinflammatoryboweldisease AT allanmmowat themannosereceptorcd206identifiesapopulationofcolonicmacrophagesinhealthandinflammatoryboweldisease AT slaterlclay themannosereceptorcd206identifiesapopulationofcolonicmacrophagesinhealthandinflammatoryboweldisease AT elainevrobertson themannosereceptorcd206identifiesapopulationofcolonicmacrophagesinhealthandinflammatoryboweldisease AT frasermorton themannosereceptorcd206identifiesapopulationofcolonicmacrophagesinhealthandinflammatoryboweldisease AT jagtarsinghnijjar themannosereceptorcd206identifiesapopulationofcolonicmacrophagesinhealthandinflammatoryboweldisease AT umerzijaz themannosereceptorcd206identifiesapopulationofcolonicmacrophagesinhealthandinflammatoryboweldisease AT simonwfmilling themannosereceptorcd206identifiesapopulationofcolonicmacrophagesinhealthandinflammatoryboweldisease AT danielrgaya themannosereceptorcd206identifiesapopulationofcolonicmacrophagesinhealthandinflammatoryboweldisease AT pamelabwright mannosereceptorcd206identifiesapopulationofcolonicmacrophagesinhealthandinflammatoryboweldisease AT elizabethmcdonald mannosereceptorcd206identifiesapopulationofcolonicmacrophagesinhealthandinflammatoryboweldisease AT albertobravoblas mannosereceptorcd206identifiesapopulationofcolonicmacrophagesinhealthandinflammatoryboweldisease AT hannahmbaer mannosereceptorcd206identifiesapopulationofcolonicmacrophagesinhealthandinflammatoryboweldisease AT annaheawood mannosereceptorcd206identifiesapopulationofcolonicmacrophagesinhealthandinflammatoryboweldisease AT calumcbain mannosereceptorcd206identifiesapopulationofcolonicmacrophagesinhealthandinflammatoryboweldisease AT allanmmowat mannosereceptorcd206identifiesapopulationofcolonicmacrophagesinhealthandinflammatoryboweldisease AT slaterlclay mannosereceptorcd206identifiesapopulationofcolonicmacrophagesinhealthandinflammatoryboweldisease AT elainevrobertson mannosereceptorcd206identifiesapopulationofcolonicmacrophagesinhealthandinflammatoryboweldisease AT frasermorton mannosereceptorcd206identifiesapopulationofcolonicmacrophagesinhealthandinflammatoryboweldisease AT jagtarsinghnijjar mannosereceptorcd206identifiesapopulationofcolonicmacrophagesinhealthandinflammatoryboweldisease AT umerzijaz mannosereceptorcd206identifiesapopulationofcolonicmacrophagesinhealthandinflammatoryboweldisease AT simonwfmilling mannosereceptorcd206identifiesapopulationofcolonicmacrophagesinhealthandinflammatoryboweldisease AT danielrgaya mannosereceptorcd206identifiesapopulationofcolonicmacrophagesinhealthandinflammatoryboweldisease
_version_	1718382623229739008

The mannose receptor (CD206) identifies a population of colonic macrophages in health and inflammatory bowel disease

Ejemplares similares