Efficacy of Thai indigenous entomopathogenic nematodes for controlling fall armyworm (Spodoptera frugiperda) (J. E. Smith)(Lepidoptera; Noctuidae)

Abstract Background Under laboratory and greenhouse conditions, the virulence of 2 isolates of Thai indigenous entomopathogenic nematodes (EPNs) in controlling the fall armyworm (FAW), Spodoptera frugiperda (J. E. Smith) (Lepidoptera; Noctuidae), was demonstrated. Six EPNs dosages were tested agains...

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Autores principales:	Wandee Wattanachaiyingcharoen, Ongpo Lepcha, Apichat Vitta, Det Wattanachaiyingcharoen
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Spodoptera frugiperda Indigenous entomopathogenic nematodes Heterorhabditis indica Steinernema siamkayai Virulence Agriculture S
Acceso en línea:	https://doaj.org/article/4a3a361394d34401bafac2b3658e8bd6
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spelling	oai:doaj.org-article:4a3a361394d34401bafac2b3658e8bd62021-11-28T12:06:19ZEfficacy of Thai indigenous entomopathogenic nematodes for controlling fall armyworm (Spodoptera frugiperda) (J. E. Smith)(Lepidoptera; Noctuidae)10.1186/s41938-021-00497-02536-9342https://doaj.org/article/4a3a361394d34401bafac2b3658e8bd62021-11-01T00:00:00Zhttps://doi.org/10.1186/s41938-021-00497-0https://doaj.org/toc/2536-9342Abstract Background Under laboratory and greenhouse conditions, the virulence of 2 isolates of Thai indigenous entomopathogenic nematodes (EPNs) in controlling the fall armyworm (FAW), Spodoptera frugiperda (J. E. Smith) (Lepidoptera; Noctuidae), was demonstrated. Six EPNs dosages were tested against 2 larval instars of FAW under the laboratory conditions, while 2 different concentrations were tested under the greenhouse conditions. Results The results of a laboratory experiment revealed that 2 Thai indigenous EPNs isolates (Heterorhabditis indica isolate AUT 13.2 and Steinernema siamkayai isolate APL 12.3) were efficient against the FAW, 2nd and 5th larval instars. Six different nematode concentrations (50,100, 150, 200, 250 and 300 infectious juveniles (IJs) ml−1) were evaluated, and all were proven to be effective, with the mortality rate associated with concentration. Inoculated larvae in the 2nd instar was more vulnerable than that in the 5th instar. H. indica isolate AUT 13.2 was more destructive than S. siamkayai isolate APL 12.3. The greatest mortality rate of 2nd instar larvae was 83% when H. indica AUT 13.2 was applied at the concentration of 250 IJs ml−1, and 68% when the nematode S. siamkayai APL 12.3 was used at the concentration of 300 IJs ml−1. At 250 IJsml−1, the highest mortality rate of the 5th instar larvae was 45% for H. indica AUT 13.2 and 33% for S. siamkayai APL 12.3, respectively. To customize the concentration and volume of nematodes suspension evaluated in the greenhouse settings, the most sensitive stage of FAW and the optimum concentration that caused the highest mortality were used. The concentrations of both indigenous nematodes’ isolates were 20,000 and 50,000 IJsml−1 per pot, respectively, and the results showed that the mortality rates were lower than that in the laboratory. FAW mortality rate was the highest (58%) in case of the nematode H. indica isolate AUT 13.2, against (45%) in case of S. siamkayai isolate APL 12.3, at the 50,000 IJs ml−1 concentrations. Conclusions The study revealed the 2 Thai indigenous EPNs isolates (H. indica isolate AUT 13.2 and S. siamkayai isolate APL 12.3) were capable of controlling the FAW in both laboratory and greenhouse environments. The 2 Thai EPNs showed the potential to be considered as a biological control agent.Wandee WattanachaiyingcharoenOngpo LepchaApichat VittaDet WattanachaiyingcharoenSpringerOpenarticleSpodoptera frugiperdaIndigenous entomopathogenic nematodesHeterorhabditis indicaSteinernema siamkayaiVirulenceAgricultureSENEgyptian Journal of Biological Pest Control, Vol 31, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Spodoptera frugiperda Indigenous entomopathogenic nematodes Heterorhabditis indica Steinernema siamkayai Virulence Agriculture S
spellingShingle	Spodoptera frugiperda Indigenous entomopathogenic nematodes Heterorhabditis indica Steinernema siamkayai Virulence Agriculture S Wandee Wattanachaiyingcharoen Ongpo Lepcha Apichat Vitta Det Wattanachaiyingcharoen Efficacy of Thai indigenous entomopathogenic nematodes for controlling fall armyworm (Spodoptera frugiperda) (J. E. Smith)(Lepidoptera; Noctuidae)
description	Abstract Background Under laboratory and greenhouse conditions, the virulence of 2 isolates of Thai indigenous entomopathogenic nematodes (EPNs) in controlling the fall armyworm (FAW), Spodoptera frugiperda (J. E. Smith) (Lepidoptera; Noctuidae), was demonstrated. Six EPNs dosages were tested against 2 larval instars of FAW under the laboratory conditions, while 2 different concentrations were tested under the greenhouse conditions. Results The results of a laboratory experiment revealed that 2 Thai indigenous EPNs isolates (Heterorhabditis indica isolate AUT 13.2 and Steinernema siamkayai isolate APL 12.3) were efficient against the FAW, 2nd and 5th larval instars. Six different nematode concentrations (50,100, 150, 200, 250 and 300 infectious juveniles (IJs) ml−1) were evaluated, and all were proven to be effective, with the mortality rate associated with concentration. Inoculated larvae in the 2nd instar was more vulnerable than that in the 5th instar. H. indica isolate AUT 13.2 was more destructive than S. siamkayai isolate APL 12.3. The greatest mortality rate of 2nd instar larvae was 83% when H. indica AUT 13.2 was applied at the concentration of 250 IJs ml−1, and 68% when the nematode S. siamkayai APL 12.3 was used at the concentration of 300 IJs ml−1. At 250 IJsml−1, the highest mortality rate of the 5th instar larvae was 45% for H. indica AUT 13.2 and 33% for S. siamkayai APL 12.3, respectively. To customize the concentration and volume of nematodes suspension evaluated in the greenhouse settings, the most sensitive stage of FAW and the optimum concentration that caused the highest mortality were used. The concentrations of both indigenous nematodes’ isolates were 20,000 and 50,000 IJsml−1 per pot, respectively, and the results showed that the mortality rates were lower than that in the laboratory. FAW mortality rate was the highest (58%) in case of the nematode H. indica isolate AUT 13.2, against (45%) in case of S. siamkayai isolate APL 12.3, at the 50,000 IJs ml−1 concentrations. Conclusions The study revealed the 2 Thai indigenous EPNs isolates (H. indica isolate AUT 13.2 and S. siamkayai isolate APL 12.3) were capable of controlling the FAW in both laboratory and greenhouse environments. The 2 Thai EPNs showed the potential to be considered as a biological control agent.
format	article
author	Wandee Wattanachaiyingcharoen Ongpo Lepcha Apichat Vitta Det Wattanachaiyingcharoen
author_facet	Wandee Wattanachaiyingcharoen Ongpo Lepcha Apichat Vitta Det Wattanachaiyingcharoen
author_sort	Wandee Wattanachaiyingcharoen
title	Efficacy of Thai indigenous entomopathogenic nematodes for controlling fall armyworm (Spodoptera frugiperda) (J. E. Smith)(Lepidoptera; Noctuidae)
title_short	Efficacy of Thai indigenous entomopathogenic nematodes for controlling fall armyworm (Spodoptera frugiperda) (J. E. Smith)(Lepidoptera; Noctuidae)
title_full	Efficacy of Thai indigenous entomopathogenic nematodes for controlling fall armyworm (Spodoptera frugiperda) (J. E. Smith)(Lepidoptera; Noctuidae)
title_fullStr	Efficacy of Thai indigenous entomopathogenic nematodes for controlling fall armyworm (Spodoptera frugiperda) (J. E. Smith)(Lepidoptera; Noctuidae)
title_full_unstemmed	Efficacy of Thai indigenous entomopathogenic nematodes for controlling fall armyworm (Spodoptera frugiperda) (J. E. Smith)(Lepidoptera; Noctuidae)
title_sort	efficacy of thai indigenous entomopathogenic nematodes for controlling fall armyworm (spodoptera frugiperda) (j. e. smith)(lepidoptera; noctuidae)
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/4a3a361394d34401bafac2b3658e8bd6
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Efficacy of Thai indigenous entomopathogenic nematodes for controlling fall armyworm (Spodoptera frugiperda) (J. E. Smith)(Lepidoptera; Noctuidae)

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