Modulatory effect of Persea Americana oil against diethylnitrosamine-induced hepatotoxicity in rats: a proposed mechanism

Abstract Background The purpose of this study was to investigate the effectiveness of Persea Americana (avocado) oil against diethylnitrosamine (DEN)-induced hepatotoxicity in rats. Methods For the induction of hepatotoxicity, DEN was administrated orally in a dose of 20 mg/kg B.wt for 6 successive...

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Autores principales:	Omayma A. R. Abozaid, Lobna M. Anees, Gehan R. Abdel-Hamed
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Hepatotoxicity Persea Americana (avocado) oil PARP-1 NADPH oxidase iNOS Apoptotic biomarkers Science Q
Acceso en línea:	https://doaj.org/article/4a45084e93d046f193a7f21ee9cf2127
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spelling	oai:doaj.org-article:4a45084e93d046f193a7f21ee9cf21272021-11-21T12:39:15ZModulatory effect of Persea Americana oil against diethylnitrosamine-induced hepatotoxicity in rats: a proposed mechanism10.1186/s42269-021-00653-42522-8307https://doaj.org/article/4a45084e93d046f193a7f21ee9cf21272021-11-01T00:00:00Zhttps://doi.org/10.1186/s42269-021-00653-4https://doaj.org/toc/2522-8307Abstract Background The purpose of this study was to investigate the effectiveness of Persea Americana (avocado) oil against diethylnitrosamine (DEN)-induced hepatotoxicity in rats. Methods For the induction of hepatotoxicity, DEN was administrated orally in a dose of 20 mg/kg B.wt for 6 successive weeks, and then the animals were gavaged with Persea Americana oil in a dose of 4 mL/kg b.wt. daily for another 6 weeks. Serum caspase-3 activity and poly (ADP-ribose) polymerase-1 (PARP-1) levels were estimated; in addition to gene expressions for NADPH oxidase, inducible nitric oxide synthase (iNOS), Bcl-2, and Bax were detected. Results The DEN-intoxicated group exhibited a remarkable increase in NADPH oxidase and iNOS expression combined with over-activation of PARP-1 and increased antiapoptotic Bcl-2 gene expression, whereas the expression of apoptotic biomarkers significantly decreased. On the other hand, treatment with Persea Americana oil significantly suppressed the elevated levels of hepatic enzymes and improved histopathological alterations in the liver. Furthermore, these groups displayed marked downregulation in NADPH oxidase and iNOS expressions. Persea Americana oil suppressed the expression of the antiapoptotic Bcl-2, activated the intrinsic mitochondrial apoptosis pathway through upregulation of pro-apoptotic Bax, and induced an obvious increase in caspase-3 activity. Moreover, Persea Americana oil administration markedly inhibited the activity of PARP-1. Conclusions This study indicated the promising potential of Persea Americana oil against DEN-induced hepatic injury through its anti-oxidative activity and pro-apoptotic effect via caspase activation and PARP-1 inhibition.Omayma A. R. AbozaidLobna M. AneesGehan R. Abdel-HamedSpringerOpenarticleHepatotoxicityPersea Americana (avocado) oilPARP-1NADPH oxidaseiNOSApoptotic biomarkersScienceQENBulletin of the National Research Centre, Vol 45, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Hepatotoxicity Persea Americana (avocado) oil PARP-1 NADPH oxidase iNOS Apoptotic biomarkers Science Q
spellingShingle	Hepatotoxicity Persea Americana (avocado) oil PARP-1 NADPH oxidase iNOS Apoptotic biomarkers Science Q Omayma A. R. Abozaid Lobna M. Anees Gehan R. Abdel-Hamed Modulatory effect of Persea Americana oil against diethylnitrosamine-induced hepatotoxicity in rats: a proposed mechanism
description	Abstract Background The purpose of this study was to investigate the effectiveness of Persea Americana (avocado) oil against diethylnitrosamine (DEN)-induced hepatotoxicity in rats. Methods For the induction of hepatotoxicity, DEN was administrated orally in a dose of 20 mg/kg B.wt for 6 successive weeks, and then the animals were gavaged with Persea Americana oil in a dose of 4 mL/kg b.wt. daily for another 6 weeks. Serum caspase-3 activity and poly (ADP-ribose) polymerase-1 (PARP-1) levels were estimated; in addition to gene expressions for NADPH oxidase, inducible nitric oxide synthase (iNOS), Bcl-2, and Bax were detected. Results The DEN-intoxicated group exhibited a remarkable increase in NADPH oxidase and iNOS expression combined with over-activation of PARP-1 and increased antiapoptotic Bcl-2 gene expression, whereas the expression of apoptotic biomarkers significantly decreased. On the other hand, treatment with Persea Americana oil significantly suppressed the elevated levels of hepatic enzymes and improved histopathological alterations in the liver. Furthermore, these groups displayed marked downregulation in NADPH oxidase and iNOS expressions. Persea Americana oil suppressed the expression of the antiapoptotic Bcl-2, activated the intrinsic mitochondrial apoptosis pathway through upregulation of pro-apoptotic Bax, and induced an obvious increase in caspase-3 activity. Moreover, Persea Americana oil administration markedly inhibited the activity of PARP-1. Conclusions This study indicated the promising potential of Persea Americana oil against DEN-induced hepatic injury through its anti-oxidative activity and pro-apoptotic effect via caspase activation and PARP-1 inhibition.
format	article
author	Omayma A. R. Abozaid Lobna M. Anees Gehan R. Abdel-Hamed
author_facet	Omayma A. R. Abozaid Lobna M. Anees Gehan R. Abdel-Hamed
author_sort	Omayma A. R. Abozaid
title	Modulatory effect of Persea Americana oil against diethylnitrosamine-induced hepatotoxicity in rats: a proposed mechanism
title_short	Modulatory effect of Persea Americana oil against diethylnitrosamine-induced hepatotoxicity in rats: a proposed mechanism
title_full	Modulatory effect of Persea Americana oil against diethylnitrosamine-induced hepatotoxicity in rats: a proposed mechanism
title_fullStr	Modulatory effect of Persea Americana oil against diethylnitrosamine-induced hepatotoxicity in rats: a proposed mechanism
title_full_unstemmed	Modulatory effect of Persea Americana oil against diethylnitrosamine-induced hepatotoxicity in rats: a proposed mechanism
title_sort	modulatory effect of persea americana oil against diethylnitrosamine-induced hepatotoxicity in rats: a proposed mechanism
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/4a45084e93d046f193a7f21ee9cf2127
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Modulatory effect of Persea Americana oil against diethylnitrosamine-induced hepatotoxicity in rats: a proposed mechanism

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