Development of an efficient Tef-1α RNA hairpin structure to efficient management of Lasiodiplodia theobromae and Neofusicoccum parvum

Abstract Lasiodiplodia theobromae and Neofusicoccum parvum are serious worldwide-distributed plant pathogenic fungi with a wide host range in tropical and temperate climates. They cause fruit rot, canker, and dieback of twigs in various woody plants. Protection of pruning wounds using fungicides is...

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Autores principales:	Omid Nili, Abdolbaset Azizi, Jafar Abdollahzadeh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4a46b5a9952d4ceda033b95f0494cc40
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spelling	oai:doaj.org-article:4a46b5a9952d4ceda033b95f0494cc402021-12-02T15:37:58ZDevelopment of an efficient Tef-1α RNA hairpin structure to efficient management of Lasiodiplodia theobromae and Neofusicoccum parvum10.1038/s41598-021-88422-12045-2322https://doaj.org/article/4a46b5a9952d4ceda033b95f0494cc402021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88422-1https://doaj.org/toc/2045-2322Abstract Lasiodiplodia theobromae and Neofusicoccum parvum are serious worldwide-distributed plant pathogenic fungi with a wide host range in tropical and temperate climates. They cause fruit rot, canker, and dieback of twigs in various woody plants. Protection of pruning wounds using fungicides is the prevalent strategy for the management of the diseases caused by these fungi. Chemical control of plant diseases is not environmentally safe and the residues of fungicides are a threat to nature. Furthermore, genetic resources of resistance to plant diseases in woody plants are limited. The aim of this study was to investigate the efficiency of RNA silencing using an efficient hairpin structure based on Tef-1α gene for the management of L. theobromae and N. parvum. Hairpin structure of Tef-1α was cloned in pFGC5941 binary vector and the recombinant construct was named pFGC-TEF-d. Transient expression of pFGC-TEF-d using Agrobacterium LBA4404 in grapevine (Bidaneh Sefid cv.) and strawberry cultivars (Camarosa and Ventana) led to a reduction in disease progress of L. theobromae. The disease reduction in grapevine was estimated by 55% and in strawberries cultivars Camarosa and Ventana by 58% and 93%, respectively. Further analysis of transient expression of pFGC-TEF-d in strawberry (Camarosa) shown disease reduction using Neofusicoccum parvum. Here we introduce RNAi silencing using pFGC-TEF-d construct as an efficient strategy to the management of L. theobromae and N. parvum for the first time.Omid NiliAbdolbaset AziziJafar AbdollahzadehNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Omid Nili Abdolbaset Azizi Jafar Abdollahzadeh Development of an efficient Tef-1α RNA hairpin structure to efficient management of Lasiodiplodia theobromae and Neofusicoccum parvum
description	Abstract Lasiodiplodia theobromae and Neofusicoccum parvum are serious worldwide-distributed plant pathogenic fungi with a wide host range in tropical and temperate climates. They cause fruit rot, canker, and dieback of twigs in various woody plants. Protection of pruning wounds using fungicides is the prevalent strategy for the management of the diseases caused by these fungi. Chemical control of plant diseases is not environmentally safe and the residues of fungicides are a threat to nature. Furthermore, genetic resources of resistance to plant diseases in woody plants are limited. The aim of this study was to investigate the efficiency of RNA silencing using an efficient hairpin structure based on Tef-1α gene for the management of L. theobromae and N. parvum. Hairpin structure of Tef-1α was cloned in pFGC5941 binary vector and the recombinant construct was named pFGC-TEF-d. Transient expression of pFGC-TEF-d using Agrobacterium LBA4404 in grapevine (Bidaneh Sefid cv.) and strawberry cultivars (Camarosa and Ventana) led to a reduction in disease progress of L. theobromae. The disease reduction in grapevine was estimated by 55% and in strawberries cultivars Camarosa and Ventana by 58% and 93%, respectively. Further analysis of transient expression of pFGC-TEF-d in strawberry (Camarosa) shown disease reduction using Neofusicoccum parvum. Here we introduce RNAi silencing using pFGC-TEF-d construct as an efficient strategy to the management of L. theobromae and N. parvum for the first time.
format	article
author	Omid Nili Abdolbaset Azizi Jafar Abdollahzadeh
author_facet	Omid Nili Abdolbaset Azizi Jafar Abdollahzadeh
author_sort	Omid Nili
title	Development of an efficient Tef-1α RNA hairpin structure to efficient management of Lasiodiplodia theobromae and Neofusicoccum parvum
title_short	Development of an efficient Tef-1α RNA hairpin structure to efficient management of Lasiodiplodia theobromae and Neofusicoccum parvum
title_full	Development of an efficient Tef-1α RNA hairpin structure to efficient management of Lasiodiplodia theobromae and Neofusicoccum parvum
title_fullStr	Development of an efficient Tef-1α RNA hairpin structure to efficient management of Lasiodiplodia theobromae and Neofusicoccum parvum
title_full_unstemmed	Development of an efficient Tef-1α RNA hairpin structure to efficient management of Lasiodiplodia theobromae and Neofusicoccum parvum
title_sort	development of an efficient tef-1α rna hairpin structure to efficient management of lasiodiplodia theobromae and neofusicoccum parvum
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4a46b5a9952d4ceda033b95f0494cc40
work_keys_str_mv	AT omidnili developmentofanefficienttef1arnahairpinstructuretoefficientmanagementoflasiodiplodiatheobromaeandneofusicoccumparvum AT abdolbasetazizi developmentofanefficienttef1arnahairpinstructuretoefficientmanagementoflasiodiplodiatheobromaeandneofusicoccumparvum AT jafarabdollahzadeh developmentofanefficienttef1arnahairpinstructuretoefficientmanagementoflasiodiplodiatheobromaeandneofusicoccumparvum
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Development of an efficient Tef-1α RNA hairpin structure to efficient management of Lasiodiplodia theobromae and Neofusicoccum parvum

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