Tumor necrosis factor-alpha (TNF-α) levels in aqueous humor of primary open angle glaucoma

Tumor necrosis factor-alpha (TNF-α) levels in aqueous humor of primary open angle glaucoma

Sankarathi Balaiya, Jayson Edwards, Tina Tillis, Vijay Khetpal, Kakarla V ChalamUniversity of Florida College of Medicine, Department of Ophthalmology Jacksonville, FL, USAPurpose: Tumor necrosis factor alpha (TNF-α), a macrophage/monocyte derived pluripotent cytokine is associated wit...

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Autores principales: Balaiya S, Edwards J, Tillis T, Khetpal V, Chalam K
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2011
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Ophthalmology
RE1-994
Acceso en línea:https://doaj.org/article/4a485f32c5b74738a88a66cd1159630e
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spelling oai:doaj.org-article:4a485f32c5b74738a88a66cd1159630e2021-12-02T05:47:24ZTumor necrosis factor-alpha (TNF-α) levels in aqueous humor of primary open angle glaucoma1177-54671177-5483https://doaj.org/article/4a485f32c5b74738a88a66cd1159630e2011-04-01T00:00:00Zhttp://www.dovepress.com/tumor-necrosis-factor-alpha-tnf-a-levels-in-aqueous-humor-of-primary-o-a7297https://doaj.org/toc/1177-5467https://doaj.org/toc/1177-5483Sankarathi Balaiya, Jayson Edwards, Tina Tillis, Vijay Khetpal, Kakarla V ChalamUniversity of Florida College of Medicine, Department of Ophthalmology Jacksonville, FL, USAPurpose: Tumor necrosis factor alpha (TNF-α), a macrophage/monocyte derived pluripotent cytokine is associated with tissue ischemia, neuronal damage and remodeling. The physiological level of TNF-α in aqueous humor of normal and glaucomatous eyes is unknown. In this study, we evaluated the TNF- α levels in aqueous in patients with primary open angle glaucoma (POAG) and compared them to controls. Methods: 50–100 µL of undiluted aqueous humor samples were obtained from eyes of 32 POAG patients who underwent cataract extraction, trabeculectomy or aqueous shunt implantation. Controls were obtained from 32 normal subjects who underwent routine cataract surgery. TNF-α levels were quantified using singleplex bead immunoassay analysis.Results: The average TNF-α level in POAG samples was 2.72 ± 1.5 pg/mL (mean ± SD). The average TNF-α level in normal samples was 1.59 ± 0.46 pg/mL (mean ± SD). Significant increase of TNF-α levels in POAG samples was noted in comparison to normal samples (P < 0.001).Conclusion: TNF-α levels are elevated in aqueous in patients with POAG compared to normal subjects based on highly sensitive Luminex® bead immunoassay and may be a reliable biomarker in the progression of glaucoma.Keywords: TNF-α, POAG, aqueous humor, cytokineBalaiya SEdwards JTillis TKhetpal VChalam KDove Medical PressarticleOphthalmologyRE1-994ENClinical Ophthalmology, Vol 2011, Iss default, Pp 553-556 (2011)
institution DOAJ
collection DOAJ
language EN
topic Ophthalmology
RE1-994
spellingShingle Ophthalmology
RE1-994
Balaiya S
Edwards J
Tillis T
Khetpal V
Chalam K
Tumor necrosis factor-alpha (TNF-α) levels in aqueous humor of primary open angle glaucoma
description Sankarathi Balaiya, Jayson Edwards, Tina Tillis, Vijay Khetpal, Kakarla V ChalamUniversity of Florida College of Medicine, Department of Ophthalmology Jacksonville, FL, USAPurpose: Tumor necrosis factor alpha (TNF-α), a macrophage/monocyte derived pluripotent cytokine is associated with tissue ischemia, neuronal damage and remodeling. The physiological level of TNF-α in aqueous humor of normal and glaucomatous eyes is unknown. In this study, we evaluated the TNF- α levels in aqueous in patients with primary open angle glaucoma (POAG) and compared them to controls. Methods: 50–100 µL of undiluted aqueous humor samples were obtained from eyes of 32 POAG patients who underwent cataract extraction, trabeculectomy or aqueous shunt implantation. Controls were obtained from 32 normal subjects who underwent routine cataract surgery. TNF-α levels were quantified using singleplex bead immunoassay analysis.Results: The average TNF-α level in POAG samples was 2.72 ± 1.5 pg/mL (mean ± SD). The average TNF-α level in normal samples was 1.59 ± 0.46 pg/mL (mean ± SD). Significant increase of TNF-α levels in POAG samples was noted in comparison to normal samples (P < 0.001).Conclusion: TNF-α levels are elevated in aqueous in patients with POAG compared to normal subjects based on highly sensitive Luminex® bead immunoassay and may be a reliable biomarker in the progression of glaucoma.Keywords: TNF-α, POAG, aqueous humor, cytokine
format article
author Balaiya S
Edwards J
Tillis T
Khetpal V
Chalam K
author_facet Balaiya S
Edwards J
Tillis T
Khetpal V
Chalam K
author_sort Balaiya S
title Tumor necrosis factor-alpha (TNF-α) levels in aqueous humor of primary open angle glaucoma
title_short Tumor necrosis factor-alpha (TNF-α) levels in aqueous humor of primary open angle glaucoma
title_full Tumor necrosis factor-alpha (TNF-α) levels in aqueous humor of primary open angle glaucoma
title_fullStr Tumor necrosis factor-alpha (TNF-α) levels in aqueous humor of primary open angle glaucoma
title_full_unstemmed Tumor necrosis factor-alpha (TNF-α) levels in aqueous humor of primary open angle glaucoma
title_sort tumor necrosis factor-alpha (tnf-α) levels in aqueous humor of primary open angle glaucoma
publisher Dove Medical Press
publishDate 2011
url https://doaj.org/article/4a485f32c5b74738a88a66cd1159630e
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