MiR-23b-3p reduces the proliferation, migration and invasion of cervical cancer cell lines via the reduction of c-Met expression
Abstract Malignant transformation and progression in cancer is associated with the altered expression of multiple miRNAs, which are considered as post-transcriptional regulators of genes participating in various cellular processes. Although, it has been proposed that miR-23b-3p acts as a tumor suppr...
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Nature Portfolio
2020
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oai:doaj.org-article:4a4db1ae5a294b72bb66e0f10be890652021-12-02T10:59:18ZMiR-23b-3p reduces the proliferation, migration and invasion of cervical cancer cell lines via the reduction of c-Met expression10.1038/s41598-020-60143-x2045-2322https://doaj.org/article/4a4db1ae5a294b72bb66e0f10be890652020-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-60143-xhttps://doaj.org/toc/2045-2322Abstract Malignant transformation and progression in cancer is associated with the altered expression of multiple miRNAs, which are considered as post-transcriptional regulators of genes participating in various cellular processes. Although, it has been proposed that miR-23b-3p acts as a tumor suppressor in cervical cancer (CC), not all the pathways through which it alters the cellular processes have been described. The present study examines whether miR-23b-3p directly represses the c-Met expression and that consequently modifies the proliferation, migration and invasion of C33A and CaSki cells. c-Met has five microRNA response elements (MREs) for miR-23b-3p in the 3′-UTR region. The ectopic overexpression of miR-23b-3p significantly reduces c-Met expression in C33A and CaSki cells. The overexpression of miR-23b-3p reduces proliferation, migration and invasion of CaSki cells and the proliferation and invasion in C33A cells. In CaSki cells, the activation of Gab1 and Fak, downstream of c-Met, is reduced in response to the overexpression of miR-23b-3p. Together, the results in the present study indicate that miR-23b-3p is a tumor suppressor that modulates the progression of CC via post-transcriptional regulation of the c-Met oncogene.Gabriela Elizabeth Campos-ViguriOscar Peralta-ZaragozaHilda Jiménez-WencesAlma Edith Longinos-GonzálezCarlos Alberto Castañón-SánchezMiriam Ramírez-CarrilloCésar López CamarilloEduardo Castañeda-SaucedoMarco Antonio Jiménez-LópezDinorah Nashely Martínez-CarrilloGloria Fernández-TilapaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-14 (2020) |
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Medicine R Science Q Gabriela Elizabeth Campos-Viguri Oscar Peralta-Zaragoza Hilda Jiménez-Wences Alma Edith Longinos-González Carlos Alberto Castañón-Sánchez Miriam Ramírez-Carrillo César López Camarillo Eduardo Castañeda-Saucedo Marco Antonio Jiménez-López Dinorah Nashely Martínez-Carrillo Gloria Fernández-Tilapa MiR-23b-3p reduces the proliferation, migration and invasion of cervical cancer cell lines via the reduction of c-Met expression |
| description |
Abstract Malignant transformation and progression in cancer is associated with the altered expression of multiple miRNAs, which are considered as post-transcriptional regulators of genes participating in various cellular processes. Although, it has been proposed that miR-23b-3p acts as a tumor suppressor in cervical cancer (CC), not all the pathways through which it alters the cellular processes have been described. The present study examines whether miR-23b-3p directly represses the c-Met expression and that consequently modifies the proliferation, migration and invasion of C33A and CaSki cells. c-Met has five microRNA response elements (MREs) for miR-23b-3p in the 3′-UTR region. The ectopic overexpression of miR-23b-3p significantly reduces c-Met expression in C33A and CaSki cells. The overexpression of miR-23b-3p reduces proliferation, migration and invasion of CaSki cells and the proliferation and invasion in C33A cells. In CaSki cells, the activation of Gab1 and Fak, downstream of c-Met, is reduced in response to the overexpression of miR-23b-3p. Together, the results in the present study indicate that miR-23b-3p is a tumor suppressor that modulates the progression of CC via post-transcriptional regulation of the c-Met oncogene. |
| format |
article |
| author |
Gabriela Elizabeth Campos-Viguri Oscar Peralta-Zaragoza Hilda Jiménez-Wences Alma Edith Longinos-González Carlos Alberto Castañón-Sánchez Miriam Ramírez-Carrillo César López Camarillo Eduardo Castañeda-Saucedo Marco Antonio Jiménez-López Dinorah Nashely Martínez-Carrillo Gloria Fernández-Tilapa |
| author_facet |
Gabriela Elizabeth Campos-Viguri Oscar Peralta-Zaragoza Hilda Jiménez-Wences Alma Edith Longinos-González Carlos Alberto Castañón-Sánchez Miriam Ramírez-Carrillo César López Camarillo Eduardo Castañeda-Saucedo Marco Antonio Jiménez-López Dinorah Nashely Martínez-Carrillo Gloria Fernández-Tilapa |
| author_sort |
Gabriela Elizabeth Campos-Viguri |
| title |
MiR-23b-3p reduces the proliferation, migration and invasion of cervical cancer cell lines via the reduction of c-Met expression |
| title_short |
MiR-23b-3p reduces the proliferation, migration and invasion of cervical cancer cell lines via the reduction of c-Met expression |
| title_full |
MiR-23b-3p reduces the proliferation, migration and invasion of cervical cancer cell lines via the reduction of c-Met expression |
| title_fullStr |
MiR-23b-3p reduces the proliferation, migration and invasion of cervical cancer cell lines via the reduction of c-Met expression |
| title_full_unstemmed |
MiR-23b-3p reduces the proliferation, migration and invasion of cervical cancer cell lines via the reduction of c-Met expression |
| title_sort |
mir-23b-3p reduces the proliferation, migration and invasion of cervical cancer cell lines via the reduction of c-met expression |
| publisher |
Nature Portfolio |
| publishDate |
2020 |
| url |
https://doaj.org/article/4a4db1ae5a294b72bb66e0f10be89065 |
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