Phases of cortical actomyosin dynamics coupled to the neuroblast polarity cycle

The Par complex dynamically polarizes to the apical cortex of asymmetrically dividing Drosophila neuroblasts where it directs fate determinant segregation. Previously, we showed that apically directed cortical movements that polarize the Par complex require F-actin (Oon and Prehoda, 2019). Here, we...

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Autores principales:	Chet Huan Oon, Kenneth E Prehoda
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	cell polarity actomyosin stem cells differentiation Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/4a4feff74aec43b4b4a3ef6744b3a073
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id	oai:doaj.org-article:4a4feff74aec43b4b4a3ef6744b3a073
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spelling	oai:doaj.org-article:4a4feff74aec43b4b4a3ef6744b3a0732021-12-03T08:56:41ZPhases of cortical actomyosin dynamics coupled to the neuroblast polarity cycle10.7554/eLife.665742050-084Xe66574https://doaj.org/article/4a4feff74aec43b4b4a3ef6744b3a0732021-11-01T00:00:00Zhttps://elifesciences.org/articles/66574https://doaj.org/toc/2050-084XThe Par complex dynamically polarizes to the apical cortex of asymmetrically dividing Drosophila neuroblasts where it directs fate determinant segregation. Previously, we showed that apically directed cortical movements that polarize the Par complex require F-actin (Oon and Prehoda, 2019). Here, we report the discovery of cortical actomyosin dynamics that begin in interphase when the Par complex is cytoplasmic but ultimately become tightly coupled to cortical Par dynamics. Interphase cortical actomyosin dynamics are unoriented and pulsatile but rapidly become sustained and apically-directed in early mitosis when the Par protein aPKC accumulates on the cortex. Apical actomyosin flows drive the coalescence of aPKC into an apical cap that depolarizes in anaphase when the flow reverses direction. Together with the previously characterized role of anaphase flows in specifying daughter cell size asymmetry, our results indicate that multiple phases of cortical actomyosin dynamics regulate asymmetric cell division.Chet Huan OonKenneth E PrehodaeLife Sciences Publications Ltdarticlecell polarityactomyosinstem cellsdifferentiationMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cell polarity actomyosin stem cells differentiation Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	cell polarity actomyosin stem cells differentiation Medicine R Science Q Biology (General) QH301-705.5 Chet Huan Oon Kenneth E Prehoda Phases of cortical actomyosin dynamics coupled to the neuroblast polarity cycle
description	The Par complex dynamically polarizes to the apical cortex of asymmetrically dividing Drosophila neuroblasts where it directs fate determinant segregation. Previously, we showed that apically directed cortical movements that polarize the Par complex require F-actin (Oon and Prehoda, 2019). Here, we report the discovery of cortical actomyosin dynamics that begin in interphase when the Par complex is cytoplasmic but ultimately become tightly coupled to cortical Par dynamics. Interphase cortical actomyosin dynamics are unoriented and pulsatile but rapidly become sustained and apically-directed in early mitosis when the Par protein aPKC accumulates on the cortex. Apical actomyosin flows drive the coalescence of aPKC into an apical cap that depolarizes in anaphase when the flow reverses direction. Together with the previously characterized role of anaphase flows in specifying daughter cell size asymmetry, our results indicate that multiple phases of cortical actomyosin dynamics regulate asymmetric cell division.
format	article
author	Chet Huan Oon Kenneth E Prehoda
author_facet	Chet Huan Oon Kenneth E Prehoda
author_sort	Chet Huan Oon
title	Phases of cortical actomyosin dynamics coupled to the neuroblast polarity cycle
title_short	Phases of cortical actomyosin dynamics coupled to the neuroblast polarity cycle
title_full	Phases of cortical actomyosin dynamics coupled to the neuroblast polarity cycle
title_fullStr	Phases of cortical actomyosin dynamics coupled to the neuroblast polarity cycle
title_full_unstemmed	Phases of cortical actomyosin dynamics coupled to the neuroblast polarity cycle
title_sort	phases of cortical actomyosin dynamics coupled to the neuroblast polarity cycle
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/4a4feff74aec43b4b4a3ef6744b3a073
work_keys_str_mv	AT chethuanoon phasesofcorticalactomyosindynamicscoupledtotheneuroblastpolaritycycle AT kennetheprehoda phasesofcorticalactomyosindynamicscoupledtotheneuroblastpolaritycycle
_version_	1718373386751574016

Phases of cortical actomyosin dynamics coupled to the neuroblast polarity cycle

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