The Dynamics of Mycoplasma gallisepticum Nucleoid Structure at the Exponential and Stationary Growth Phases

The structure and dynamics of bacterial nucleoids play important roles in regulating gene expression. Bacteria of class Mollicutes and, in particular, mycoplasmas feature extremely reduced genomes. They lack multiple structural proteins of the nucleoid, as well as regulators of gene expression. We s...

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Autores principales:	Gleb Y. Fisunov, Alexander I. Zubov, Olga V. Pobeguts, Anna M. Varizhuk, Mariya A. Galyamina, Daria V. Evsyutina, Tatiana A. Semashko, Valentin A. Manuvera, Sergey I. Kovalchuk, Rustam K. Ziganshin, Nicolay A. Barinov, Dmitry V. Klinov, Vadim M. Govorun
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Mycoplasma gallisepticum nucleoid nucleoid-associated proteins enolase proteome Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/4a563cb35058458e8c8863042553d8d0
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spelling	oai:doaj.org-article:4a563cb35058458e8c8863042553d8d02021-11-18T08:42:27ZThe Dynamics of Mycoplasma gallisepticum Nucleoid Structure at the Exponential and Stationary Growth Phases1664-302X10.3389/fmicb.2021.753760https://doaj.org/article/4a563cb35058458e8c8863042553d8d02021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.753760/fullhttps://doaj.org/toc/1664-302XThe structure and dynamics of bacterial nucleoids play important roles in regulating gene expression. Bacteria of class Mollicutes and, in particular, mycoplasmas feature extremely reduced genomes. They lack multiple structural proteins of the nucleoid, as well as regulators of gene expression. We studied the organization of Mycoplasma gallisepticum nucleoids in the stationary and exponential growth phases at the structural and protein levels. The growth phase transition results in the structural reorganization of M. gallisepticum nucleoid. In particular, it undergoes condensation and changes in the protein content. The observed changes corroborate with the previously identified global rearrangement of the transcriptional landscape in this bacterium during the growth phase transition. In addition, we identified that the glycolytic enzyme enolase functions as a nucleoid structural protein in this bacterium. It is capable of non-specific DNA binding and can form fibril-like complexes with DNA.Gleb Y. FisunovAlexander I. ZubovOlga V. PobegutsAnna M. VarizhukMariya A. GalyaminaDaria V. EvsyutinaTatiana A. SemashkoValentin A. ManuveraSergey I. KovalchukRustam K. ZiganshinNicolay A. BarinovDmitry V. KlinovVadim M. GovorunFrontiers Media S.A.articleMycoplasma gallisepticumnucleoidnucleoid-associated proteinsenolaseproteomeMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Mycoplasma gallisepticum nucleoid nucleoid-associated proteins enolase proteome Microbiology QR1-502
spellingShingle	Mycoplasma gallisepticum nucleoid nucleoid-associated proteins enolase proteome Microbiology QR1-502 Gleb Y. Fisunov Alexander I. Zubov Olga V. Pobeguts Anna M. Varizhuk Mariya A. Galyamina Daria V. Evsyutina Tatiana A. Semashko Valentin A. Manuvera Sergey I. Kovalchuk Rustam K. Ziganshin Nicolay A. Barinov Dmitry V. Klinov Vadim M. Govorun The Dynamics of Mycoplasma gallisepticum Nucleoid Structure at the Exponential and Stationary Growth Phases
description	The structure and dynamics of bacterial nucleoids play important roles in regulating gene expression. Bacteria of class Mollicutes and, in particular, mycoplasmas feature extremely reduced genomes. They lack multiple structural proteins of the nucleoid, as well as regulators of gene expression. We studied the organization of Mycoplasma gallisepticum nucleoids in the stationary and exponential growth phases at the structural and protein levels. The growth phase transition results in the structural reorganization of M. gallisepticum nucleoid. In particular, it undergoes condensation and changes in the protein content. The observed changes corroborate with the previously identified global rearrangement of the transcriptional landscape in this bacterium during the growth phase transition. In addition, we identified that the glycolytic enzyme enolase functions as a nucleoid structural protein in this bacterium. It is capable of non-specific DNA binding and can form fibril-like complexes with DNA.
format	article
author	Gleb Y. Fisunov Alexander I. Zubov Olga V. Pobeguts Anna M. Varizhuk Mariya A. Galyamina Daria V. Evsyutina Tatiana A. Semashko Valentin A. Manuvera Sergey I. Kovalchuk Rustam K. Ziganshin Nicolay A. Barinov Dmitry V. Klinov Vadim M. Govorun
author_facet	Gleb Y. Fisunov Alexander I. Zubov Olga V. Pobeguts Anna M. Varizhuk Mariya A. Galyamina Daria V. Evsyutina Tatiana A. Semashko Valentin A. Manuvera Sergey I. Kovalchuk Rustam K. Ziganshin Nicolay A. Barinov Dmitry V. Klinov Vadim M. Govorun
author_sort	Gleb Y. Fisunov
title	The Dynamics of Mycoplasma gallisepticum Nucleoid Structure at the Exponential and Stationary Growth Phases
title_short	The Dynamics of Mycoplasma gallisepticum Nucleoid Structure at the Exponential and Stationary Growth Phases
title_full	The Dynamics of Mycoplasma gallisepticum Nucleoid Structure at the Exponential and Stationary Growth Phases
title_fullStr	The Dynamics of Mycoplasma gallisepticum Nucleoid Structure at the Exponential and Stationary Growth Phases
title_full_unstemmed	The Dynamics of Mycoplasma gallisepticum Nucleoid Structure at the Exponential and Stationary Growth Phases
title_sort	dynamics of mycoplasma gallisepticum nucleoid structure at the exponential and stationary growth phases
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/4a563cb35058458e8c8863042553d8d0
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The Dynamics of Mycoplasma gallisepticum Nucleoid Structure at the Exponential and Stationary Growth Phases

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