Reliable DNA barcoding performance proved for species and island populations of comoran squamate reptiles.

In the past decade, DNA barcoding became increasingly common as a method for species identification in biodiversity inventories and related studies. However, mainly due to technical obstacles, squamate reptiles have been the target of few barcoding studies. In this article, we present the results of...

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Autores principales:	Oliver Hawlitschek, Zoltán T Nagy, Johannes Berger, Frank Glaw
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4a5997ed9b334b878eb103bfba78ad89
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spelling	oai:doaj.org-article:4a5997ed9b334b878eb103bfba78ad892021-11-18T08:55:35ZReliable DNA barcoding performance proved for species and island populations of comoran squamate reptiles.1932-620310.1371/journal.pone.0073368https://doaj.org/article/4a5997ed9b334b878eb103bfba78ad892013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24069192/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203In the past decade, DNA barcoding became increasingly common as a method for species identification in biodiversity inventories and related studies. However, mainly due to technical obstacles, squamate reptiles have been the target of few barcoding studies. In this article, we present the results of a DNA barcoding study of squamates of the Comoros archipelago, a poorly studied group of oceanic islands close to and mostly colonized from Madagascar. The barcoding dataset presented here includes 27 of the 29 currently recognized squamate species of the Comoros, including 17 of the 18 endemic species. Some species considered endemic to the Comoros according to current taxonomy were found to cluster with non-Comoran lineages, probably due to poorly resolved taxonomy. All other species for which more than one barcode was obtained corresponded to distinct clusters useful for species identification by barcoding. In most species, even island populations could be distinguished using barcoding. Two cryptic species were identified using the DNA barcoding approach. The obtained barcoding topology, a Bayesian tree based on COI sequences of 5 genera, was compared with available multigene topologies, and in 3 cases, major incongruences between the two topologies became evident. Three of the multigene studies were initiated after initial screening of a preliminary version of the barcoding dataset presented here. We conclude that in the case of the squamates of the Comoros Islands, DNA barcoding has proven a very useful and efficient way of detecting isolated populations and promising starting points for subsequent research.Oliver HawlitschekZoltán T NagyJohannes BergerFrank GlawPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e73368 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Oliver Hawlitschek Zoltán T Nagy Johannes Berger Frank Glaw Reliable DNA barcoding performance proved for species and island populations of comoran squamate reptiles.
description	In the past decade, DNA barcoding became increasingly common as a method for species identification in biodiversity inventories and related studies. However, mainly due to technical obstacles, squamate reptiles have been the target of few barcoding studies. In this article, we present the results of a DNA barcoding study of squamates of the Comoros archipelago, a poorly studied group of oceanic islands close to and mostly colonized from Madagascar. The barcoding dataset presented here includes 27 of the 29 currently recognized squamate species of the Comoros, including 17 of the 18 endemic species. Some species considered endemic to the Comoros according to current taxonomy were found to cluster with non-Comoran lineages, probably due to poorly resolved taxonomy. All other species for which more than one barcode was obtained corresponded to distinct clusters useful for species identification by barcoding. In most species, even island populations could be distinguished using barcoding. Two cryptic species were identified using the DNA barcoding approach. The obtained barcoding topology, a Bayesian tree based on COI sequences of 5 genera, was compared with available multigene topologies, and in 3 cases, major incongruences between the two topologies became evident. Three of the multigene studies were initiated after initial screening of a preliminary version of the barcoding dataset presented here. We conclude that in the case of the squamates of the Comoros Islands, DNA barcoding has proven a very useful and efficient way of detecting isolated populations and promising starting points for subsequent research.
format	article
author	Oliver Hawlitschek Zoltán T Nagy Johannes Berger Frank Glaw
author_facet	Oliver Hawlitschek Zoltán T Nagy Johannes Berger Frank Glaw
author_sort	Oliver Hawlitschek
title	Reliable DNA barcoding performance proved for species and island populations of comoran squamate reptiles.
title_short	Reliable DNA barcoding performance proved for species and island populations of comoran squamate reptiles.
title_full	Reliable DNA barcoding performance proved for species and island populations of comoran squamate reptiles.
title_fullStr	Reliable DNA barcoding performance proved for species and island populations of comoran squamate reptiles.
title_full_unstemmed	Reliable DNA barcoding performance proved for species and island populations of comoran squamate reptiles.
title_sort	reliable dna barcoding performance proved for species and island populations of comoran squamate reptiles.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/4a5997ed9b334b878eb103bfba78ad89
work_keys_str_mv	AT oliverhawlitschek reliablednabarcodingperformanceprovedforspeciesandislandpopulationsofcomoransquamatereptiles AT zoltantnagy reliablednabarcodingperformanceprovedforspeciesandislandpopulationsofcomoransquamatereptiles AT johannesberger reliablednabarcodingperformanceprovedforspeciesandislandpopulationsofcomoransquamatereptiles AT frankglaw reliablednabarcodingperformanceprovedforspeciesandislandpopulationsofcomoransquamatereptiles
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Reliable DNA barcoding performance proved for species and island populations of comoran squamate reptiles.

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