Culturable gut bacteria of Ikan Batak (Neolissochilus sumatranus Weber & de Beaufort, 1916) collected in Toba Samosir, Indonesia

Abstract. Dinoto A, Handayani R, Setianingrum N, Julistiono H. 2020. Culturable gut bacteria of Ikan Batak (Neolissochilus sumatranus Weber & de Beaufort, 1916) collected in Toba Samosir, Indonesia. Biodiversitas 21: 4483-4488. Ikan Batak (Neolissochilus sumatranus Weber & de Beaufort, 1916)...

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Autores principales:	Achmad Dinoto, Rini Handayani, Ninu Setianingrum, Heddy Julistiono
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	bacillus, gut bacteria, ikan batak, klebsiella, lactobacillus, neolissochilus sumatranus, tumebacillus Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/4a5e25ecebdd472eb5663879c8f0ed04
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spelling	oai:doaj.org-article:4a5e25ecebdd472eb5663879c8f0ed042021-11-22T00:51:09ZCulturable gut bacteria of Ikan Batak (Neolissochilus sumatranus Weber & de Beaufort, 1916) collected in Toba Samosir, Indonesia1412-033X2085-472210.13057/biodiv/d211003https://doaj.org/article/4a5e25ecebdd472eb5663879c8f0ed042020-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6481https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Dinoto A, Handayani R, Setianingrum N, Julistiono H. 2020. Culturable gut bacteria of Ikan Batak (Neolissochilus sumatranus Weber & de Beaufort, 1916) collected in Toba Samosir, Indonesia. Biodiversitas 21: 4483-4488. Ikan Batak (Neolissochilus sumatranus Weber & de Beaufort, 1916) is one of the fish species that is rarely found in water and have the status of endangered species. In consequence, the loss of endemic fish may contribute to the loss of microorganisms that inhabits the fish as a host. The studies on microorganisms associated with N. sumatranus are very limited. Therefore, the purpose of this study was to isolate and identify the culturable bacteria isolated from the gut of N. sumatranus. Sampling of N. sumatranus was carried out in a river within Toba Samosir area which flows to Lake Toba. Fish gut content was collected for isolating microorganisms using three media, including MRS, 10X diluted MRS, and MRS supplemented with 1% bile salt. Thirteen isolates were successfully isolated and identified based on 16S rRNA. This study revealed various species of gut bacteria recovered from N. sumatranus based on BLAST analysis. The isolates showed closest relationship to species Bacillus subtilis (3 isolates), Bacillus tequilensis (2 isolates), Tumebacillus ginsengisoli (6 isolates), Klebsiella pneumoniae (1 isolate), and Lactobacillus pentosus (1 isolate) with the similarity ranging at 98.7 to 100%. All 16S rRNA gene nucleotides of isolates have been submitted to GenBank. This study also described the isolates that have a very close relationship with Bacillus tequilla and Bacillus subtilis. Further identification is challenged to obtain a big picture of the diversity of microorganisms and the functionality in the digestive ecosystem of N. sumatranus for their conservation and bioprospecting of microbial-based aquaculture.Achmad DinotoRini HandayaniNinu SetianingrumHeddy JulistionoMBI & UNS Soloarticlebacillus, gut bacteria, ikan batak, klebsiella, lactobacillus, neolissochilus sumatranus, tumebacillusBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacillus, gut bacteria, ikan batak, klebsiella, lactobacillus, neolissochilus sumatranus, tumebacillus Biology (General) QH301-705.5
spellingShingle	bacillus, gut bacteria, ikan batak, klebsiella, lactobacillus, neolissochilus sumatranus, tumebacillus Biology (General) QH301-705.5 Achmad Dinoto Rini Handayani Ninu Setianingrum Heddy Julistiono Culturable gut bacteria of Ikan Batak (Neolissochilus sumatranus Weber & de Beaufort, 1916) collected in Toba Samosir, Indonesia
description	Abstract. Dinoto A, Handayani R, Setianingrum N, Julistiono H. 2020. Culturable gut bacteria of Ikan Batak (Neolissochilus sumatranus Weber & de Beaufort, 1916) collected in Toba Samosir, Indonesia. Biodiversitas 21: 4483-4488. Ikan Batak (Neolissochilus sumatranus Weber & de Beaufort, 1916) is one of the fish species that is rarely found in water and have the status of endangered species. In consequence, the loss of endemic fish may contribute to the loss of microorganisms that inhabits the fish as a host. The studies on microorganisms associated with N. sumatranus are very limited. Therefore, the purpose of this study was to isolate and identify the culturable bacteria isolated from the gut of N. sumatranus. Sampling of N. sumatranus was carried out in a river within Toba Samosir area which flows to Lake Toba. Fish gut content was collected for isolating microorganisms using three media, including MRS, 10X diluted MRS, and MRS supplemented with 1% bile salt. Thirteen isolates were successfully isolated and identified based on 16S rRNA. This study revealed various species of gut bacteria recovered from N. sumatranus based on BLAST analysis. The isolates showed closest relationship to species Bacillus subtilis (3 isolates), Bacillus tequilensis (2 isolates), Tumebacillus ginsengisoli (6 isolates), Klebsiella pneumoniae (1 isolate), and Lactobacillus pentosus (1 isolate) with the similarity ranging at 98.7 to 100%. All 16S rRNA gene nucleotides of isolates have been submitted to GenBank. This study also described the isolates that have a very close relationship with Bacillus tequilla and Bacillus subtilis. Further identification is challenged to obtain a big picture of the diversity of microorganisms and the functionality in the digestive ecosystem of N. sumatranus for their conservation and bioprospecting of microbial-based aquaculture.
format	article
author	Achmad Dinoto Rini Handayani Ninu Setianingrum Heddy Julistiono
author_facet	Achmad Dinoto Rini Handayani Ninu Setianingrum Heddy Julistiono
author_sort	Achmad Dinoto
title	Culturable gut bacteria of Ikan Batak (Neolissochilus sumatranus Weber & de Beaufort, 1916) collected in Toba Samosir, Indonesia
title_short	Culturable gut bacteria of Ikan Batak (Neolissochilus sumatranus Weber & de Beaufort, 1916) collected in Toba Samosir, Indonesia
title_full	Culturable gut bacteria of Ikan Batak (Neolissochilus sumatranus Weber & de Beaufort, 1916) collected in Toba Samosir, Indonesia
title_fullStr	Culturable gut bacteria of Ikan Batak (Neolissochilus sumatranus Weber & de Beaufort, 1916) collected in Toba Samosir, Indonesia
title_full_unstemmed	Culturable gut bacteria of Ikan Batak (Neolissochilus sumatranus Weber & de Beaufort, 1916) collected in Toba Samosir, Indonesia
title_sort	culturable gut bacteria of ikan batak (neolissochilus sumatranus weber & de beaufort, 1916) collected in toba samosir, indonesia
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/4a5e25ecebdd472eb5663879c8f0ed04
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_version_	1718418458060783616

Culturable gut bacteria of Ikan Batak (Neolissochilus sumatranus Weber & de Beaufort, 1916) collected in Toba Samosir, Indonesia

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