Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes

Abstract A novel polysaccharide FVPB2 was purified from fruiting bodies of Flammulina velutipes. Its structure was elucidated by monosaccharide composition and methylation analyses, UV-Visible and FTIR spectroscopy as well as NMR. FVPB2 was a homogeneous heteropolysaccharide (molecular weight ~ 1.50...

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Autores principales:	Wen-Han Wang, Jing-Song Zhang, Ting Feng, Jing Deng, Chi-Chung Lin, Hua Fan, Wen-Juan Yu, Hai-Ying Bao, Wei Jia
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4a65c0f0f3b9482194de13e0df1b6500
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spelling	oai:doaj.org-article:4a65c0f0f3b9482194de13e0df1b65002021-12-02T15:08:41ZStructural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes10.1038/s41598-018-21375-02045-2322https://doaj.org/article/4a65c0f0f3b9482194de13e0df1b65002018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-21375-0https://doaj.org/toc/2045-2322Abstract A novel polysaccharide FVPB2 was purified from fruiting bodies of Flammulina velutipes. Its structure was elucidated by monosaccharide composition and methylation analyses, UV-Visible and FTIR spectroscopy as well as NMR. FVPB2 was a homogeneous heteropolysaccharide (molecular weight ~ 1.50 × 104 Da) containing D-galactose, D-mannose, L-fucose, and D-glucose at molar ratio of 1.9:1.2:1:2.5. In vitro immunomodulatory studies showed FVPB2 induced proliferation of mouse spleen lymphocytes in a dose-dependent manner. The levels of IgM and IgG, secreted by B cells, increased after FVPB2 treatment. So FVPB2 has potential to be a new important immunomodulatory nutraceutical.Wen-Han WangJing-Song ZhangTing FengJing DengChi-Chung LinHua FanWen-Juan YuHai-Ying BaoWei JiaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wen-Han Wang Jing-Song Zhang Ting Feng Jing Deng Chi-Chung Lin Hua Fan Wen-Juan Yu Hai-Ying Bao Wei Jia Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes
description	Abstract A novel polysaccharide FVPB2 was purified from fruiting bodies of Flammulina velutipes. Its structure was elucidated by monosaccharide composition and methylation analyses, UV-Visible and FTIR spectroscopy as well as NMR. FVPB2 was a homogeneous heteropolysaccharide (molecular weight ~ 1.50 × 104 Da) containing D-galactose, D-mannose, L-fucose, and D-glucose at molar ratio of 1.9:1.2:1:2.5. In vitro immunomodulatory studies showed FVPB2 induced proliferation of mouse spleen lymphocytes in a dose-dependent manner. The levels of IgM and IgG, secreted by B cells, increased after FVPB2 treatment. So FVPB2 has potential to be a new important immunomodulatory nutraceutical.
format	article
author	Wen-Han Wang Jing-Song Zhang Ting Feng Jing Deng Chi-Chung Lin Hua Fan Wen-Juan Yu Hai-Ying Bao Wei Jia
author_facet	Wen-Han Wang Jing-Song Zhang Ting Feng Jing Deng Chi-Chung Lin Hua Fan Wen-Juan Yu Hai-Ying Bao Wei Jia
author_sort	Wen-Han Wang
title	Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes
title_short	Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes
title_full	Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes
title_fullStr	Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes
title_full_unstemmed	Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes
title_sort	structural elucidation of a polysaccharide from flammulina velutipes and its immunomodulation activities on mouse b lymphocytes
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/4a65c0f0f3b9482194de13e0df1b6500
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Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes

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