Physiology of the Nitrite-Oxidizing Bacterium Candidatus Nitrotoga sp. CP45 Enriched From a Colorado River

Nitrogen cycling microbes, including nitrite-oxidizing bacteria (NOB), perform critical ecosystem functions that help mitigate anthropogenic stresses and maintain ecosystem health. Activity of these beneficial nitrogen cycling microbes is dictated in part by the microorganisms’ response to physicoch...

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Autores principales:	Munira A. Lantz, Andrew M. Boddicker, Michael P. Kain, Owen M. C. Berg, Courtney D. Wham, Annika C. Mosier
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	nitrite-oxidizing bacteria freshwater nitrification water quality nitrotoga antibiotics Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/4a681b5c032c493a833bed095abeb3e9
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spelling	oai:doaj.org-article:4a681b5c032c493a833bed095abeb3e92021-11-12T17:10:53ZPhysiology of the Nitrite-Oxidizing Bacterium Candidatus Nitrotoga sp. CP45 Enriched From a Colorado River1664-302X10.3389/fmicb.2021.709371https://doaj.org/article/4a681b5c032c493a833bed095abeb3e92021-08-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.709371/fullhttps://doaj.org/toc/1664-302XNitrogen cycling microbes, including nitrite-oxidizing bacteria (NOB), perform critical ecosystem functions that help mitigate anthropogenic stresses and maintain ecosystem health. Activity of these beneficial nitrogen cycling microbes is dictated in part by the microorganisms’ response to physicochemical conditions, such as temperature, pH, and nutrient availability. NOB from the newly described Candidatus Nitrotoga genus have been detected in a wide range of habitats across the globe, yet only a few organisms within the genus have been physiologically characterized. For freshwater systems where NOB are critical for supporting aquatic life, Ca. Nitrotoga have been previously detected but little is known about the physiological potential of these organisms or their response to changing environmental conditions. Here, we determined functional response to environmental change for a representative freshwater species of Ca. Nitrotoga (Ca. Nitrotoga sp. CP45, enriched from a Colorado river). The physiological findings demonstrated that CP45 maintained nitrite oxidation at pH levels of 5–8, at temperatures from 4 to 28°C, and when incubated in the dark. Light exposure and elevated temperature (30°C) completely halted nitrite oxidation. Ca. Nitrotoga sp. CP45 maintained nitrite oxidation upon exposure to four different antibiotics, and potential rates of nitrite oxidation by river sediment communities were also resilient to antibiotic stress. We explored the Ca. Nitrotoga sp. CP45 genome to make predictions about adaptations to enable survival under specific conditions. Overall, these results contribute to our understanding of the versatility of a representative freshwater Ca. Nitrotoga sp. Identifying the specific environmental conditions that maximize NOB metabolic rates may ultimately direct future management decisions aimed at restoring impacted systems.Munira A. LantzAndrew M. BoddickerMichael P. KainOwen M. C. BergCourtney D. WhamAnnika C. MosierFrontiers Media S.A.articlenitrite-oxidizing bacteriafreshwaternitrificationwater qualitynitrotogaantibioticsMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	nitrite-oxidizing bacteria freshwater nitrification water quality nitrotoga antibiotics Microbiology QR1-502
spellingShingle	nitrite-oxidizing bacteria freshwater nitrification water quality nitrotoga antibiotics Microbiology QR1-502 Munira A. Lantz Andrew M. Boddicker Michael P. Kain Owen M. C. Berg Courtney D. Wham Annika C. Mosier Physiology of the Nitrite-Oxidizing Bacterium Candidatus Nitrotoga sp. CP45 Enriched From a Colorado River
description	Nitrogen cycling microbes, including nitrite-oxidizing bacteria (NOB), perform critical ecosystem functions that help mitigate anthropogenic stresses and maintain ecosystem health. Activity of these beneficial nitrogen cycling microbes is dictated in part by the microorganisms’ response to physicochemical conditions, such as temperature, pH, and nutrient availability. NOB from the newly described Candidatus Nitrotoga genus have been detected in a wide range of habitats across the globe, yet only a few organisms within the genus have been physiologically characterized. For freshwater systems where NOB are critical for supporting aquatic life, Ca. Nitrotoga have been previously detected but little is known about the physiological potential of these organisms or their response to changing environmental conditions. Here, we determined functional response to environmental change for a representative freshwater species of Ca. Nitrotoga (Ca. Nitrotoga sp. CP45, enriched from a Colorado river). The physiological findings demonstrated that CP45 maintained nitrite oxidation at pH levels of 5–8, at temperatures from 4 to 28°C, and when incubated in the dark. Light exposure and elevated temperature (30°C) completely halted nitrite oxidation. Ca. Nitrotoga sp. CP45 maintained nitrite oxidation upon exposure to four different antibiotics, and potential rates of nitrite oxidation by river sediment communities were also resilient to antibiotic stress. We explored the Ca. Nitrotoga sp. CP45 genome to make predictions about adaptations to enable survival under specific conditions. Overall, these results contribute to our understanding of the versatility of a representative freshwater Ca. Nitrotoga sp. Identifying the specific environmental conditions that maximize NOB metabolic rates may ultimately direct future management decisions aimed at restoring impacted systems.
format	article
author	Munira A. Lantz Andrew M. Boddicker Michael P. Kain Owen M. C. Berg Courtney D. Wham Annika C. Mosier
author_facet	Munira A. Lantz Andrew M. Boddicker Michael P. Kain Owen M. C. Berg Courtney D. Wham Annika C. Mosier
author_sort	Munira A. Lantz
title	Physiology of the Nitrite-Oxidizing Bacterium Candidatus Nitrotoga sp. CP45 Enriched From a Colorado River
title_short	Physiology of the Nitrite-Oxidizing Bacterium Candidatus Nitrotoga sp. CP45 Enriched From a Colorado River
title_full	Physiology of the Nitrite-Oxidizing Bacterium Candidatus Nitrotoga sp. CP45 Enriched From a Colorado River
title_fullStr	Physiology of the Nitrite-Oxidizing Bacterium Candidatus Nitrotoga sp. CP45 Enriched From a Colorado River
title_full_unstemmed	Physiology of the Nitrite-Oxidizing Bacterium Candidatus Nitrotoga sp. CP45 Enriched From a Colorado River
title_sort	physiology of the nitrite-oxidizing bacterium candidatus nitrotoga sp. cp45 enriched from a colorado river
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/4a681b5c032c493a833bed095abeb3e9
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Physiology of the Nitrite-Oxidizing Bacterium Candidatus Nitrotoga sp. CP45 Enriched From a Colorado River

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