Transforming protein-polymer conjugate purification by tuning protein solubility

Transforming protein-polymer conjugate purification by tuning protein solubility

Therapeutic proteins are often conjugated with polymers, but separating the conjugate from unconjugated protein and free polymer is a major challenge. Here, the authors discover that proteins conjugated to charged or zwitterionic polymers maintain solubility in 100% ammonium sulfate, greatly simplif...

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Autores principales: Stefanie L. Baker, Aravinda Munasinghe, Bibifatima Kaupbayeva, Nin Rebecca Kang, Marie Certiat, Hironobu Murata, Krzysztof Matyjaszewski, Ping Lin, Coray M. Colina, Alan J. Russell
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Science
Q
Acceso en línea:https://doaj.org/article/4a69909d08314ec1b598d1eaa7136811
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spelling oai:doaj.org-article:4a69909d08314ec1b598d1eaa71368112021-12-02T15:35:40ZTransforming protein-polymer conjugate purification by tuning protein solubility10.1038/s41467-019-12612-92041-1723https://doaj.org/article/4a69909d08314ec1b598d1eaa71368112019-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-12612-9https://doaj.org/toc/2041-1723Therapeutic proteins are often conjugated with polymers, but separating the conjugate from unconjugated protein and free polymer is a major challenge. Here, the authors discover that proteins conjugated to charged or zwitterionic polymers maintain solubility in 100% ammonium sulfate, greatly simplifying purification.Stefanie L. BakerAravinda MunasingheBibifatima KaupbayevaNin Rebecca KangMarie CertiatHironobu MurataKrzysztof MatyjaszewskiPing LinCoray M. ColinaAlan J. RussellNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-12 (2019)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Stefanie L. Baker
Aravinda Munasinghe
Bibifatima Kaupbayeva
Nin Rebecca Kang
Marie Certiat
Hironobu Murata
Krzysztof Matyjaszewski
Ping Lin
Coray M. Colina
Alan J. Russell
Transforming protein-polymer conjugate purification by tuning protein solubility
description Therapeutic proteins are often conjugated with polymers, but separating the conjugate from unconjugated protein and free polymer is a major challenge. Here, the authors discover that proteins conjugated to charged or zwitterionic polymers maintain solubility in 100% ammonium sulfate, greatly simplifying purification.
format article
author Stefanie L. Baker
Aravinda Munasinghe
Bibifatima Kaupbayeva
Nin Rebecca Kang
Marie Certiat
Hironobu Murata
Krzysztof Matyjaszewski
Ping Lin
Coray M. Colina
Alan J. Russell
author_facet Stefanie L. Baker
Aravinda Munasinghe
Bibifatima Kaupbayeva
Nin Rebecca Kang
Marie Certiat
Hironobu Murata
Krzysztof Matyjaszewski
Ping Lin
Coray M. Colina
Alan J. Russell
author_sort Stefanie L. Baker
title Transforming protein-polymer conjugate purification by tuning protein solubility
title_short Transforming protein-polymer conjugate purification by tuning protein solubility
title_full Transforming protein-polymer conjugate purification by tuning protein solubility
title_fullStr Transforming protein-polymer conjugate purification by tuning protein solubility
title_full_unstemmed Transforming protein-polymer conjugate purification by tuning protein solubility
title_sort transforming protein-polymer conjugate purification by tuning protein solubility
publisher Nature Portfolio
publishDate 2019
url https://doaj.org/article/4a69909d08314ec1b598d1eaa7136811
work_keys_str_mv AT stefanielbaker transformingproteinpolymerconjugatepurificationbytuningproteinsolubility
AT aravindamunasinghe transformingproteinpolymerconjugatepurificationbytuningproteinsolubility
AT bibifatimakaupbayeva transformingproteinpolymerconjugatepurificationbytuningproteinsolubility
AT ninrebeccakang transformingproteinpolymerconjugatepurificationbytuningproteinsolubility
AT mariecertiat transformingproteinpolymerconjugatepurificationbytuningproteinsolubility
AT hironobumurata transformingproteinpolymerconjugatepurificationbytuningproteinsolubility
AT krzysztofmatyjaszewski transformingproteinpolymerconjugatepurificationbytuningproteinsolubility
AT pinglin transformingproteinpolymerconjugatepurificationbytuningproteinsolubility
AT coraymcolina transformingproteinpolymerconjugatepurificationbytuningproteinsolubility
AT alanjrussell transformingproteinpolymerconjugatepurificationbytuningproteinsolubility
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