Assessment of the Effects of Cyclooxygenase Inhibitors on the Immune Status Following Surgery in Adult Male Rats

Background and Objectives: Surgery via different mechanisms causes immunosuppression in the postoperative period. The aim of the present study was to investigate the effects of preoperative administration of cyclooxygenase inhibitors on blood levels of Interleukin-2 (IL-2), Interferon-γ (IFN-γ), Tum...

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Autores principales:	Mohammad Abyari, Samad Alimohammadi, Mehrdad Pooyanmehr, Ali Ghashghaii, Ali Maleki
Formato:	article
Lenguaje:	FA
Publicado:	Qom University of Medical Sciences 2021
Materias:	immune system cyclooxygenase inhibitors surgery rat Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4a69940263034ec685581b2bbd902798
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spelling	oai:doaj.org-article:4a69940263034ec685581b2bbd9027982021-12-05T11:45:05ZAssessment of the Effects of Cyclooxygenase Inhibitors on the Immune Status Following Surgery in Adult Male Rats1735-77992008-1375https://doaj.org/article/4a69940263034ec685581b2bbd9027982021-04-01T00:00:00Zhttp://journal.muq.ac.ir/article-1-3124-en.htmlhttps://doaj.org/toc/1735-7799https://doaj.org/toc/2008-1375Background and Objectives: Surgery via different mechanisms causes immunosuppression in the postoperative period. The aim of the present study was to investigate the effects of preoperative administration of cyclooxygenase inhibitors on blood levels of Interleukin-2 (IL-2), Interferon-γ (IFN-γ), Tumor Necrosis Factor-α (TNF-α), white blood cells (WBCs) and lymphocytes. Methods: Seventy-five male Wistar rats were divided into five groups (n=15 for each group). Group 1 was gavaged with normal saline for 3 consecutive days without surgery. Group 2 was gavaged with normal saline for 3 consecutive days. Groups 3 to 5 were gavaged with indomethacin (4 mg/kg) and celecoxib (7.5 and 15 mg/kg), respectively, for 3 consecutive days. In Groups 2 to 5, one hour after drugs administration on the third day, laparotomy was used as a surgery pattern. Blood samples were collected 24 hours after surgery and levels IL-2, IFN-γ, TNF-α, WBCs and lymphocytes were determined. Data were analyzed using one-way ANOVA test. Results: Based on the results, a decrease in blood levels of IL-2, IFN-γ and lymphocytes along with an increase in TNF-α and WBCs were observed 24 hours after surgery compared to control rats (P<0.05). Indomethacin (4 mg/kg) and celecoxib (15 mg/kg) improved the decreased level of IL-2 (P<0.05). There was no significant difference in IFN-γ and lymphocytes between surgery group and the groups treated with indomethacin or celecoxib (P>0.05). Also, only celecoxib (15 mg/kg) could decrease the elevated levels TNF-α and WBCs (P<0.05). Conclusion: Perioperative use of cyclooxygenase inhibitors can improve immune system function.Mohammad AbyariSamad AlimohammadiMehrdad PooyanmehrAli GhashghaiiAli MalekiQom University of Medical Sciencesarticleimmune systemcyclooxygenase inhibitorssurgeryratMedicine (General)R5-920FAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum, Vol 15, Iss 2, Pp 110-119 (2021)
institution	DOAJ
collection	DOAJ
language	FA
topic	immune system cyclooxygenase inhibitors surgery rat Medicine (General) R5-920
spellingShingle	immune system cyclooxygenase inhibitors surgery rat Medicine (General) R5-920 Mohammad Abyari Samad Alimohammadi Mehrdad Pooyanmehr Ali Ghashghaii Ali Maleki Assessment of the Effects of Cyclooxygenase Inhibitors on the Immune Status Following Surgery in Adult Male Rats
description	Background and Objectives: Surgery via different mechanisms causes immunosuppression in the postoperative period. The aim of the present study was to investigate the effects of preoperative administration of cyclooxygenase inhibitors on blood levels of Interleukin-2 (IL-2), Interferon-γ (IFN-γ), Tumor Necrosis Factor-α (TNF-α), white blood cells (WBCs) and lymphocytes. Methods: Seventy-five male Wistar rats were divided into five groups (n=15 for each group). Group 1 was gavaged with normal saline for 3 consecutive days without surgery. Group 2 was gavaged with normal saline for 3 consecutive days. Groups 3 to 5 were gavaged with indomethacin (4 mg/kg) and celecoxib (7.5 and 15 mg/kg), respectively, for 3 consecutive days. In Groups 2 to 5, one hour after drugs administration on the third day, laparotomy was used as a surgery pattern. Blood samples were collected 24 hours after surgery and levels IL-2, IFN-γ, TNF-α, WBCs and lymphocytes were determined. Data were analyzed using one-way ANOVA test. Results: Based on the results, a decrease in blood levels of IL-2, IFN-γ and lymphocytes along with an increase in TNF-α and WBCs were observed 24 hours after surgery compared to control rats (P<0.05). Indomethacin (4 mg/kg) and celecoxib (15 mg/kg) improved the decreased level of IL-2 (P<0.05). There was no significant difference in IFN-γ and lymphocytes between surgery group and the groups treated with indomethacin or celecoxib (P>0.05). Also, only celecoxib (15 mg/kg) could decrease the elevated levels TNF-α and WBCs (P<0.05). Conclusion: Perioperative use of cyclooxygenase inhibitors can improve immune system function.
format	article
author	Mohammad Abyari Samad Alimohammadi Mehrdad Pooyanmehr Ali Ghashghaii Ali Maleki
author_facet	Mohammad Abyari Samad Alimohammadi Mehrdad Pooyanmehr Ali Ghashghaii Ali Maleki
author_sort	Mohammad Abyari
title	Assessment of the Effects of Cyclooxygenase Inhibitors on the Immune Status Following Surgery in Adult Male Rats
title_short	Assessment of the Effects of Cyclooxygenase Inhibitors on the Immune Status Following Surgery in Adult Male Rats
title_full	Assessment of the Effects of Cyclooxygenase Inhibitors on the Immune Status Following Surgery in Adult Male Rats
title_fullStr	Assessment of the Effects of Cyclooxygenase Inhibitors on the Immune Status Following Surgery in Adult Male Rats
title_full_unstemmed	Assessment of the Effects of Cyclooxygenase Inhibitors on the Immune Status Following Surgery in Adult Male Rats
title_sort	assessment of the effects of cyclooxygenase inhibitors on the immune status following surgery in adult male rats
publisher	Qom University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/4a69940263034ec685581b2bbd902798
work_keys_str_mv	AT mohammadabyari assessmentoftheeffectsofcyclooxygenaseinhibitorsontheimmunestatusfollowingsurgeryinadultmalerats AT samadalimohammadi assessmentoftheeffectsofcyclooxygenaseinhibitorsontheimmunestatusfollowingsurgeryinadultmalerats AT mehrdadpooyanmehr assessmentoftheeffectsofcyclooxygenaseinhibitorsontheimmunestatusfollowingsurgeryinadultmalerats AT alighashghaii assessmentoftheeffectsofcyclooxygenaseinhibitorsontheimmunestatusfollowingsurgeryinadultmalerats AT alimaleki assessmentoftheeffectsofcyclooxygenaseinhibitorsontheimmunestatusfollowingsurgeryinadultmalerats
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Assessment of the Effects of Cyclooxygenase Inhibitors on the Immune Status Following Surgery in Adult Male Rats

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