Effects of intraperitoneally injected silver nanoparticles on histological structures and blood parameters in the albino rat

Osama Mohamed M Sarhan,1,2 Rehab M Hussein31Department of Zoology, Faculty of Sciences, Fayoum University, Al Fayoum, Egypt; 2Department of Biology, Faculty of Applied Science, Umm Al-Qura University, Makkah Al-Mukarramah, Saudi Arabia; 3Department of Zoology, Faculty of Science, Cairo University,...

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Autores principales:	Sarhan OM, Hussein RM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4a69b9c14681450697b2fa9462b1f097
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spelling	oai:doaj.org-article:4a69b9c14681450697b2fa9462b1f0972021-12-02T01:50:38ZEffects of intraperitoneally injected silver nanoparticles on histological structures and blood parameters in the albino rat1178-2013https://doaj.org/article/4a69b9c14681450697b2fa9462b1f0972014-03-01T00:00:00Zhttp://www.dovepress.com/effects-of-intraperitoneally-injected-silver-nanoparticles-on-histolog-a16200https://doaj.org/toc/1178-2013 Osama Mohamed M Sarhan,1,2 Rehab M Hussein31Department of Zoology, Faculty of Sciences, Fayoum University, Al Fayoum, Egypt; 2Department of Biology, Faculty of Applied Science, Umm Al-Qura University, Makkah Al-Mukarramah, Saudi Arabia; 3Department of Zoology, Faculty of Science, Cairo University, Giza, EgyptBackground: The purpose of this study was to investigate the effect of acute dosing with silver nanoparticles (AgNPs) and identify potential ultrastructural alterations in the liver and kidney and their effect on blood parameters in the albino rat.Methods: Twenty rats were used to assess the acute effects of AgNPs. Rats in the treatment group were injected intraperitoneally with 0.5 mL of distilled water containing AgNPs at a dose of 2,000 mg/kg body weight followed by a second injection after 48 hours. Control rats received two 0.5 mL doses of distilled water only. After 3 days, blood samples were collected, and the rat kidneys and livers were extracted and processed for electron microscopy to investigate for hematologic and histopathologic alterations.Results: Renal tubules showed swollen epithelium with cytoplasmic vacuolization, thickening of the basement membrane, and destruction of some mitochondrial cristae. Podocytes showed elongation and swelling of their primary and secondary processes. The basement membrane of the capillary tufts became thicker. The hepatic tissue showed narrowing of the sinusoids, swollen hepatocytes with hypertrophied nucleoli, and accumulation of fat globules in the nucleoplasm and cytoplasm. The hepatic sinusoids showed hypertrophied endothelial and Kupffer. Destructed cristae of some mitochondria, endosomes, and larger lysosomes filled with Ag-NPs were also observed in the Kupffer cells. Significant increases were observed in white blood cell count, lymphocyte count, granulocytes, and hemoglobin. There was a significant increase in serum creatinine, urea, and aspartate and alanine aminotransferases.Conclusion: To the best of the authors' knowledge, the ultrastructural changes in renal and liver tissue observed in this study have not been described before. Our results suggest that injection of AgNPs could have severe cytotoxic effects on the structure and function of these organs.Keywords: silver nanoparticles, kidney, liver, blood, toxicity, ratSarhan OMHussein RMDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 1505-1517 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Sarhan OM Hussein RM Effects of intraperitoneally injected silver nanoparticles on histological structures and blood parameters in the albino rat
description	Osama Mohamed M Sarhan,1,2 Rehab M Hussein31Department of Zoology, Faculty of Sciences, Fayoum University, Al Fayoum, Egypt; 2Department of Biology, Faculty of Applied Science, Umm Al-Qura University, Makkah Al-Mukarramah, Saudi Arabia; 3Department of Zoology, Faculty of Science, Cairo University, Giza, EgyptBackground: The purpose of this study was to investigate the effect of acute dosing with silver nanoparticles (AgNPs) and identify potential ultrastructural alterations in the liver and kidney and their effect on blood parameters in the albino rat.Methods: Twenty rats were used to assess the acute effects of AgNPs. Rats in the treatment group were injected intraperitoneally with 0.5 mL of distilled water containing AgNPs at a dose of 2,000 mg/kg body weight followed by a second injection after 48 hours. Control rats received two 0.5 mL doses of distilled water only. After 3 days, blood samples were collected, and the rat kidneys and livers were extracted and processed for electron microscopy to investigate for hematologic and histopathologic alterations.Results: Renal tubules showed swollen epithelium with cytoplasmic vacuolization, thickening of the basement membrane, and destruction of some mitochondrial cristae. Podocytes showed elongation and swelling of their primary and secondary processes. The basement membrane of the capillary tufts became thicker. The hepatic tissue showed narrowing of the sinusoids, swollen hepatocytes with hypertrophied nucleoli, and accumulation of fat globules in the nucleoplasm and cytoplasm. The hepatic sinusoids showed hypertrophied endothelial and Kupffer. Destructed cristae of some mitochondria, endosomes, and larger lysosomes filled with Ag-NPs were also observed in the Kupffer cells. Significant increases were observed in white blood cell count, lymphocyte count, granulocytes, and hemoglobin. There was a significant increase in serum creatinine, urea, and aspartate and alanine aminotransferases.Conclusion: To the best of the authors' knowledge, the ultrastructural changes in renal and liver tissue observed in this study have not been described before. Our results suggest that injection of AgNPs could have severe cytotoxic effects on the structure and function of these organs.Keywords: silver nanoparticles, kidney, liver, blood, toxicity, rat
format	article
author	Sarhan OM Hussein RM
author_facet	Sarhan OM Hussein RM
author_sort	Sarhan OM
title	Effects of intraperitoneally injected silver nanoparticles on histological structures and blood parameters in the albino rat
title_short	Effects of intraperitoneally injected silver nanoparticles on histological structures and blood parameters in the albino rat
title_full	Effects of intraperitoneally injected silver nanoparticles on histological structures and blood parameters in the albino rat
title_fullStr	Effects of intraperitoneally injected silver nanoparticles on histological structures and blood parameters in the albino rat
title_full_unstemmed	Effects of intraperitoneally injected silver nanoparticles on histological structures and blood parameters in the albino rat
title_sort	effects of intraperitoneally injected silver nanoparticles on histological structures and blood parameters in the albino rat
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/4a69b9c14681450697b2fa9462b1f097
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Effects of intraperitoneally injected silver nanoparticles on histological structures and blood parameters in the albino rat

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