Sea Bass Primary Cultures versus RTgill-W1 Cell Line: Influence of Cell Model on the Sensitivity to Nanoparticles

Determination of acute toxicity to vertebrates in aquatic environments is mainly performed following OECD test guideline 203, requiring the use of a large number of fish and with mortality as endpoint. This test is also used to determine toxicity of nanomaterials in aquatic environments. Since a rep...

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Autores principales:	Alba Jimeno-Romero, Frederik Gwinner, Michelle Müller, Espen Mariussen, Manu Soto, Yvonne Kohl
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	nanoparticles gills titanium dioxide silver polystyrene protein corona Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/4a6afb86cc2840b3baa70b3368ba2312
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spelling	oai:doaj.org-article:4a6afb86cc2840b3baa70b3368ba23122021-11-25T18:32:44ZSea Bass Primary Cultures versus RTgill-W1 Cell Line: Influence of Cell Model on the Sensitivity to Nanoparticles10.3390/nano111131362079-4991https://doaj.org/article/4a6afb86cc2840b3baa70b3368ba23122021-11-01T00:00:00Zhttps://www.mdpi.com/2079-4991/11/11/3136https://doaj.org/toc/2079-4991Determination of acute toxicity to vertebrates in aquatic environments is mainly performed following OECD test guideline 203, requiring the use of a large number of fish and with mortality as endpoint. This test is also used to determine toxicity of nanomaterials in aquatic environments. Since a replacement method for animal testing in nanotoxicity studies is desirable, the feasibility of fish primary cultures or cell lines as a model for nanotoxicity screenings is investigated here. <i>Dicentrarchus labrax</i> primary cultures and RTgill-W1 cell line were exposed to several concentrations (0.1 to 200 ug/mL) of different nanoparticles (TiO<sub>2</sub>, polystyrene and silver), and cytotoxicity, metabolic activity and reactive oxygen species formation were investigated after 24 and 48 h of exposure. Protein corona as amount of protein bound, as well as the influence of surface modification (-COOH, -NH<sub>2</sub>), exposure media (Leibovitz’s L15 or seawater), weathering and cell type were the experimental variables included to test their influence on the results of the assays. Data from all scenarios was split based on the significance each experimental variable had in the result of the cytotoxicity tests, in an exploratory approach that allows for better understanding of the determining factors affecting toxicity. Data shows that more variables significantly influenced the outcome of toxicity tests when the primary cultures were exposed to the different nanoparticles. Toxicity tests performed in RTgill-W1 were influenced only by exposure time and nanoparticle concentration. The whole data set was integrated in a biological response index to show the overall impact of nanoparticle exposures.Alba Jimeno-RomeroFrederik GwinnerMichelle MüllerEspen MariussenManu SotoYvonne KohlMDPI AGarticlenanoparticlesgillstitanium dioxidesilverpolystyreneprotein coronaChemistryQD1-999ENNanomaterials, Vol 11, Iss 3136, p 3136 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	nanoparticles gills titanium dioxide silver polystyrene protein corona Chemistry QD1-999
spellingShingle	nanoparticles gills titanium dioxide silver polystyrene protein corona Chemistry QD1-999 Alba Jimeno-Romero Frederik Gwinner Michelle Müller Espen Mariussen Manu Soto Yvonne Kohl Sea Bass Primary Cultures versus RTgill-W1 Cell Line: Influence of Cell Model on the Sensitivity to Nanoparticles
description	Determination of acute toxicity to vertebrates in aquatic environments is mainly performed following OECD test guideline 203, requiring the use of a large number of fish and with mortality as endpoint. This test is also used to determine toxicity of nanomaterials in aquatic environments. Since a replacement method for animal testing in nanotoxicity studies is desirable, the feasibility of fish primary cultures or cell lines as a model for nanotoxicity screenings is investigated here. <i>Dicentrarchus labrax</i> primary cultures and RTgill-W1 cell line were exposed to several concentrations (0.1 to 200 ug/mL) of different nanoparticles (TiO<sub>2</sub>, polystyrene and silver), and cytotoxicity, metabolic activity and reactive oxygen species formation were investigated after 24 and 48 h of exposure. Protein corona as amount of protein bound, as well as the influence of surface modification (-COOH, -NH<sub>2</sub>), exposure media (Leibovitz’s L15 or seawater), weathering and cell type were the experimental variables included to test their influence on the results of the assays. Data from all scenarios was split based on the significance each experimental variable had in the result of the cytotoxicity tests, in an exploratory approach that allows for better understanding of the determining factors affecting toxicity. Data shows that more variables significantly influenced the outcome of toxicity tests when the primary cultures were exposed to the different nanoparticles. Toxicity tests performed in RTgill-W1 were influenced only by exposure time and nanoparticle concentration. The whole data set was integrated in a biological response index to show the overall impact of nanoparticle exposures.
format	article
author	Alba Jimeno-Romero Frederik Gwinner Michelle Müller Espen Mariussen Manu Soto Yvonne Kohl
author_facet	Alba Jimeno-Romero Frederik Gwinner Michelle Müller Espen Mariussen Manu Soto Yvonne Kohl
author_sort	Alba Jimeno-Romero
title	Sea Bass Primary Cultures versus RTgill-W1 Cell Line: Influence of Cell Model on the Sensitivity to Nanoparticles
title_short	Sea Bass Primary Cultures versus RTgill-W1 Cell Line: Influence of Cell Model on the Sensitivity to Nanoparticles
title_full	Sea Bass Primary Cultures versus RTgill-W1 Cell Line: Influence of Cell Model on the Sensitivity to Nanoparticles
title_fullStr	Sea Bass Primary Cultures versus RTgill-W1 Cell Line: Influence of Cell Model on the Sensitivity to Nanoparticles
title_full_unstemmed	Sea Bass Primary Cultures versus RTgill-W1 Cell Line: Influence of Cell Model on the Sensitivity to Nanoparticles
title_sort	sea bass primary cultures versus rtgill-w1 cell line: influence of cell model on the sensitivity to nanoparticles
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4a6afb86cc2840b3baa70b3368ba2312
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Sea Bass Primary Cultures versus RTgill-W1 Cell Line: Influence of Cell Model on the Sensitivity to Nanoparticles

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