Protective effect of interval exercise training with different intensity and alpha-lipoic acid supplement on Nav1.3 protein in soleus muscle of diabetic rats

Introduction: Diabetes is a common metabolic disease, which leads to diabetic peripheral neuropathy. Peripheral neuron damage result in Nav1.3 elevations. Exercise training has beneficial role in diabetes management and peripheral neuropathy. Alpha lipoic acid (ALA) is a powerful biological antioxid...

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Autores principales:	Seyedeh Fatemeh Fatemi, Seyed Abdollah Hashemvarzi, Amin Farzaneh Hesari
Formato:	article
Lenguaje:	FA
Publicado:	Shahid Sadoughi University of Medical Sciences 2021
Materias:	alpha-lipoic acid exercise training nav1.3 diabet. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4a6fea4e01734247b311835b879e7627
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spelling	oai:doaj.org-article:4a6fea4e01734247b311835b879e76272021-11-15T07:41:20ZProtective effect of interval exercise training with different intensity and alpha-lipoic acid supplement on Nav1.3 protein in soleus muscle of diabetic rats2228-57412228-5733https://doaj.org/article/4a6fea4e01734247b311835b879e76272021-11-01T00:00:00Zhttp://jssu.ssu.ac.ir/article-1-5344-en.htmlhttps://doaj.org/toc/2228-5741https://doaj.org/toc/2228-5733Introduction: Diabetes is a common metabolic disease, which leads to diabetic peripheral neuropathy. Peripheral neuron damage result in Nav1.3 elevations. Exercise training has beneficial role in diabetes management and peripheral neuropathy. Alpha lipoic acid (ALA) is a powerful biological antioxidant. However, the role of exercise training and ALA on Nav1.3 are not well understood. The aim of the present study was to investigate the effect of training with different intensity and Alpha lipoic acid supplement on soleus muscle Nav1.3 protein in rats with type 2 diabetes. Thirty-five male Wistar rats were randomly divided into seven groups: healthy control, diabetic, complementary diabetic, intensive exercise diabetic, moderate exercise diabetic, intensive exercise + supplemental diabetic, moderate exercise + complementary diabetic. Methods: In this experimental study, 35 male Wistar rats were randomly divided into seven groups: healthy control, diabetic (D), complementary (alpha lipoic acid) diabetic (ALA), diabetic high intensity training (HIT), diabetic moderate intensity training (MIT), diabetes HIT+ALA (ALA + HIT), diabetic MIT + ALA (ALA + MIT). Rats were diabetic by intra-peritoneal injection of STZ. The HIT and MIT protocols were performed five days a week for six weeks. HIIT included 10 bouts of four minutes (running at 85–90% of maximum speed) and MIT 13 bouts of four minutes (running at 65–70% of maximum speed). ALA was administered orally 20 mg/kg once a day by gavage. Nav1.3 protein levels were measured by immunohistochemistry method. Statistical operations were performed with SPSS version 16 software. One-way analysis of variance and Tukey were used to analyze the data. Results: The level of Nav1.3 increased significantly in diabetic group compared to the control (p≤0.0001). Moreover, HIT (p=0.0015), MIT p=0.0056), ALA+HIT (p≤0.0001) and ALA+MIT (p≤0.0001) decreased significantly Nav1.3 compared to the diabetic group. Conclusion: HIT and MIT can reduce the expression of NaV1.3 in soleus muscle in diabetic rats. ALA combined with exercise training can be more effective to reduce diabetic neuropathy.Seyedeh Fatemeh FatemiSeyed Abdollah HashemvarziAmin Farzaneh HesariShahid Sadoughi University of Medical Sciencesarticlealpha-lipoic acidexercise trainingnav1.3diabet.Medicine (General)R5-920FAMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd, Vol 29, Iss 8, Pp 3976-3988 (2021)
institution	DOAJ
collection	DOAJ
language	FA
topic	alpha-lipoic acid exercise training nav1.3 diabet. Medicine (General) R5-920
spellingShingle	alpha-lipoic acid exercise training nav1.3 diabet. Medicine (General) R5-920 Seyedeh Fatemeh Fatemi Seyed Abdollah Hashemvarzi Amin Farzaneh Hesari Protective effect of interval exercise training with different intensity and alpha-lipoic acid supplement on Nav1.3 protein in soleus muscle of diabetic rats
description	Introduction: Diabetes is a common metabolic disease, which leads to diabetic peripheral neuropathy. Peripheral neuron damage result in Nav1.3 elevations. Exercise training has beneficial role in diabetes management and peripheral neuropathy. Alpha lipoic acid (ALA) is a powerful biological antioxidant. However, the role of exercise training and ALA on Nav1.3 are not well understood. The aim of the present study was to investigate the effect of training with different intensity and Alpha lipoic acid supplement on soleus muscle Nav1.3 protein in rats with type 2 diabetes. Thirty-five male Wistar rats were randomly divided into seven groups: healthy control, diabetic, complementary diabetic, intensive exercise diabetic, moderate exercise diabetic, intensive exercise + supplemental diabetic, moderate exercise + complementary diabetic. Methods: In this experimental study, 35 male Wistar rats were randomly divided into seven groups: healthy control, diabetic (D), complementary (alpha lipoic acid) diabetic (ALA), diabetic high intensity training (HIT), diabetic moderate intensity training (MIT), diabetes HIT+ALA (ALA + HIT), diabetic MIT + ALA (ALA + MIT). Rats were diabetic by intra-peritoneal injection of STZ. The HIT and MIT protocols were performed five days a week for six weeks. HIIT included 10 bouts of four minutes (running at 85–90% of maximum speed) and MIT 13 bouts of four minutes (running at 65–70% of maximum speed). ALA was administered orally 20 mg/kg once a day by gavage. Nav1.3 protein levels were measured by immunohistochemistry method. Statistical operations were performed with SPSS version 16 software. One-way analysis of variance and Tukey were used to analyze the data. Results: The level of Nav1.3 increased significantly in diabetic group compared to the control (p≤0.0001). Moreover, HIT (p=0.0015), MIT p=0.0056), ALA+HIT (p≤0.0001) and ALA+MIT (p≤0.0001) decreased significantly Nav1.3 compared to the diabetic group. Conclusion: HIT and MIT can reduce the expression of NaV1.3 in soleus muscle in diabetic rats. ALA combined with exercise training can be more effective to reduce diabetic neuropathy.
format	article
author	Seyedeh Fatemeh Fatemi Seyed Abdollah Hashemvarzi Amin Farzaneh Hesari
author_facet	Seyedeh Fatemeh Fatemi Seyed Abdollah Hashemvarzi Amin Farzaneh Hesari
author_sort	Seyedeh Fatemeh Fatemi
title	Protective effect of interval exercise training with different intensity and alpha-lipoic acid supplement on Nav1.3 protein in soleus muscle of diabetic rats
title_short	Protective effect of interval exercise training with different intensity and alpha-lipoic acid supplement on Nav1.3 protein in soleus muscle of diabetic rats
title_full	Protective effect of interval exercise training with different intensity and alpha-lipoic acid supplement on Nav1.3 protein in soleus muscle of diabetic rats
title_fullStr	Protective effect of interval exercise training with different intensity and alpha-lipoic acid supplement on Nav1.3 protein in soleus muscle of diabetic rats
title_full_unstemmed	Protective effect of interval exercise training with different intensity and alpha-lipoic acid supplement on Nav1.3 protein in soleus muscle of diabetic rats
title_sort	protective effect of interval exercise training with different intensity and alpha-lipoic acid supplement on nav1.3 protein in soleus muscle of diabetic rats
publisher	Shahid Sadoughi University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/4a6fea4e01734247b311835b879e7627
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Protective effect of interval exercise training with different intensity and alpha-lipoic acid supplement on Nav1.3 protein in soleus muscle of diabetic rats

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