Flutamide treatment reveals a relationship between steroidogenic activity of Leydig cells and ultrastructure of their mitochondria

Abstract Our present knowledge on interrelation between morphology/ultrastructure of mitochondria of the Leydig cell and its steroidogenic function is far from satisfactory and needs additional studies. Here, we analyzed the effects of blockade of androgen receptor, triggered by exposure to flutamid...

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Autores principales:	Malgorzata Brzoskwinia, Laura Pardyak, Alicja Kaminska, Wacław Tworzydlo, Anna Hejmej, Sylwia Marek, Szczepan M. Bilinski, Barbara Bilinska
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4a71d2da881b43479f8ab911f66dd912
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spelling	oai:doaj.org-article:4a71d2da881b43479f8ab911f66dd9122021-12-02T14:33:57ZFlutamide treatment reveals a relationship between steroidogenic activity of Leydig cells and ultrastructure of their mitochondria10.1038/s41598-021-93292-82045-2322https://doaj.org/article/4a71d2da881b43479f8ab911f66dd9122021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93292-8https://doaj.org/toc/2045-2322Abstract Our present knowledge on interrelation between morphology/ultrastructure of mitochondria of the Leydig cell and its steroidogenic function is far from satisfactory and needs additional studies. Here, we analyzed the effects of blockade of androgen receptor, triggered by exposure to flutamide, on the expression of steroidogenic proteins (1) and ultrastructure of Leydig cells’ constituents (2). We demonstrated that increase in the expression level of steroidogenic (StAR, CYP11A1, 3β-HSD, and CYP19A1) proteins (and respective mRNAs) in rat testicular tissue as well as elevation of intratesticular sex steroid hormone (testosterone and estradiol) levels observed in treated animals correspond well to morphological alterations of the Leydig cell ultrastructure. Most importantly, up-regulation of steroidogenic proteins’ expression apparently correlates with considerable multiplication of Leydig cell mitochondria and subsequent formation of local mitochondrial networks. Interestingly, we showed also that the above-mentioned processes were associated with elevated transcription of Drp1 and Mfn2 genes, encoding proteins implicated in mitochondrial dynamics. Collectively, our studies emphasize the importance of mitochondrial homeostasis to the steroidogenic function of Leydig cells.Malgorzata BrzoskwiniaLaura PardyakAlicja KaminskaWacław TworzydloAnna HejmejSylwia MarekSzczepan M. BilinskiBarbara BilinskaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Malgorzata Brzoskwinia Laura Pardyak Alicja Kaminska Wacław Tworzydlo Anna Hejmej Sylwia Marek Szczepan M. Bilinski Barbara Bilinska Flutamide treatment reveals a relationship between steroidogenic activity of Leydig cells and ultrastructure of their mitochondria
description	Abstract Our present knowledge on interrelation between morphology/ultrastructure of mitochondria of the Leydig cell and its steroidogenic function is far from satisfactory and needs additional studies. Here, we analyzed the effects of blockade of androgen receptor, triggered by exposure to flutamide, on the expression of steroidogenic proteins (1) and ultrastructure of Leydig cells’ constituents (2). We demonstrated that increase in the expression level of steroidogenic (StAR, CYP11A1, 3β-HSD, and CYP19A1) proteins (and respective mRNAs) in rat testicular tissue as well as elevation of intratesticular sex steroid hormone (testosterone and estradiol) levels observed in treated animals correspond well to morphological alterations of the Leydig cell ultrastructure. Most importantly, up-regulation of steroidogenic proteins’ expression apparently correlates with considerable multiplication of Leydig cell mitochondria and subsequent formation of local mitochondrial networks. Interestingly, we showed also that the above-mentioned processes were associated with elevated transcription of Drp1 and Mfn2 genes, encoding proteins implicated in mitochondrial dynamics. Collectively, our studies emphasize the importance of mitochondrial homeostasis to the steroidogenic function of Leydig cells.
format	article
author	Malgorzata Brzoskwinia Laura Pardyak Alicja Kaminska Wacław Tworzydlo Anna Hejmej Sylwia Marek Szczepan M. Bilinski Barbara Bilinska
author_facet	Malgorzata Brzoskwinia Laura Pardyak Alicja Kaminska Wacław Tworzydlo Anna Hejmej Sylwia Marek Szczepan M. Bilinski Barbara Bilinska
author_sort	Malgorzata Brzoskwinia
title	Flutamide treatment reveals a relationship between steroidogenic activity of Leydig cells and ultrastructure of their mitochondria
title_short	Flutamide treatment reveals a relationship between steroidogenic activity of Leydig cells and ultrastructure of their mitochondria
title_full	Flutamide treatment reveals a relationship between steroidogenic activity of Leydig cells and ultrastructure of their mitochondria
title_fullStr	Flutamide treatment reveals a relationship between steroidogenic activity of Leydig cells and ultrastructure of their mitochondria
title_full_unstemmed	Flutamide treatment reveals a relationship between steroidogenic activity of Leydig cells and ultrastructure of their mitochondria
title_sort	flutamide treatment reveals a relationship between steroidogenic activity of leydig cells and ultrastructure of their mitochondria
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4a71d2da881b43479f8ab911f66dd912
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Flutamide treatment reveals a relationship between steroidogenic activity of Leydig cells and ultrastructure of their mitochondria

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