Improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging

Super‐resolution fluorescence imaging provides critically improved information about the composition, organization, and dynamics of subcellular structures. Quantum dot triexciton imaging (QDTI) has been introduced as an easy‐to‐use sub‐diffraction imaging method that achieves an almost 2‐fold improv...

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Autores principales: Simon Hennig, Dietmar J. Manstein
Formato: article
Lenguaje:EN
Publicado: Wiley 2021
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Acceso en línea:https://doaj.org/article/4a82618becbc4a9ab6586414948d7b61
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spelling oai:doaj.org-article:4a82618becbc4a9ab6586414948d7b612021-12-01T13:36:13ZImprovement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging2211-546310.1002/2211-5463.13246https://doaj.org/article/4a82618becbc4a9ab6586414948d7b612021-12-01T00:00:00Zhttps://doi.org/10.1002/2211-5463.13246https://doaj.org/toc/2211-5463Super‐resolution fluorescence imaging provides critically improved information about the composition, organization, and dynamics of subcellular structures. Quantum dot triexciton imaging (QDTI) has been introduced as an easy‐to‐use sub‐diffraction imaging method that achieves an almost 2‐fold improvement in resolution when used with conventional confocal microscopes. Here, we report an overall 3‐fold increase in lateral and axial resolution compared to conventional confocal microscopes by combining QDTI with state‐of‐the‐art commercial laser scanning microscope systems.Simon HennigDietmar J. MansteinWileyarticlequantum dot triexciton imagingAiryscanSTELLARIS3D confocal imagingnanoscopysuper‐resolution fluorescence imagingBiology (General)QH301-705.5ENFEBS Open Bio, Vol 11, Iss 12, Pp 3324-3330 (2021)
institution DOAJ
collection DOAJ
language EN
topic quantum dot triexciton imaging
Airyscan
STELLARIS
3D confocal imaging
nanoscopy
super‐resolution fluorescence imaging
Biology (General)
QH301-705.5
spellingShingle quantum dot triexciton imaging
Airyscan
STELLARIS
3D confocal imaging
nanoscopy
super‐resolution fluorescence imaging
Biology (General)
QH301-705.5
Simon Hennig
Dietmar J. Manstein
Improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging
description Super‐resolution fluorescence imaging provides critically improved information about the composition, organization, and dynamics of subcellular structures. Quantum dot triexciton imaging (QDTI) has been introduced as an easy‐to‐use sub‐diffraction imaging method that achieves an almost 2‐fold improvement in resolution when used with conventional confocal microscopes. Here, we report an overall 3‐fold increase in lateral and axial resolution compared to conventional confocal microscopes by combining QDTI with state‐of‐the‐art commercial laser scanning microscope systems.
format article
author Simon Hennig
Dietmar J. Manstein
author_facet Simon Hennig
Dietmar J. Manstein
author_sort Simon Hennig
title Improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging
title_short Improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging
title_full Improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging
title_fullStr Improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging
title_full_unstemmed Improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging
title_sort improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging
publisher Wiley
publishDate 2021
url https://doaj.org/article/4a82618becbc4a9ab6586414948d7b61
work_keys_str_mv AT simonhennig improvementofimageresolutionbycombiningenhancedconfocalmicroscopyandquantumdottriexcitonimaging
AT dietmarjmanstein improvementofimageresolutionbycombiningenhancedconfocalmicroscopyandquantumdottriexcitonimaging
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