Improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging
Super‐resolution fluorescence imaging provides critically improved information about the composition, organization, and dynamics of subcellular structures. Quantum dot triexciton imaging (QDTI) has been introduced as an easy‐to‐use sub‐diffraction imaging method that achieves an almost 2‐fold improv...
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Wiley
2021
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oai:doaj.org-article:4a82618becbc4a9ab6586414948d7b612021-12-01T13:36:13ZImprovement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging2211-546310.1002/2211-5463.13246https://doaj.org/article/4a82618becbc4a9ab6586414948d7b612021-12-01T00:00:00Zhttps://doi.org/10.1002/2211-5463.13246https://doaj.org/toc/2211-5463Super‐resolution fluorescence imaging provides critically improved information about the composition, organization, and dynamics of subcellular structures. Quantum dot triexciton imaging (QDTI) has been introduced as an easy‐to‐use sub‐diffraction imaging method that achieves an almost 2‐fold improvement in resolution when used with conventional confocal microscopes. Here, we report an overall 3‐fold increase in lateral and axial resolution compared to conventional confocal microscopes by combining QDTI with state‐of‐the‐art commercial laser scanning microscope systems.Simon HennigDietmar J. MansteinWileyarticlequantum dot triexciton imagingAiryscanSTELLARIS3D confocal imagingnanoscopysuper‐resolution fluorescence imagingBiology (General)QH301-705.5ENFEBS Open Bio, Vol 11, Iss 12, Pp 3324-3330 (2021) |
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DOAJ |
language |
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topic |
quantum dot triexciton imaging Airyscan STELLARIS 3D confocal imaging nanoscopy super‐resolution fluorescence imaging Biology (General) QH301-705.5 |
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quantum dot triexciton imaging Airyscan STELLARIS 3D confocal imaging nanoscopy super‐resolution fluorescence imaging Biology (General) QH301-705.5 Simon Hennig Dietmar J. Manstein Improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging |
description |
Super‐resolution fluorescence imaging provides critically improved information about the composition, organization, and dynamics of subcellular structures. Quantum dot triexciton imaging (QDTI) has been introduced as an easy‐to‐use sub‐diffraction imaging method that achieves an almost 2‐fold improvement in resolution when used with conventional confocal microscopes. Here, we report an overall 3‐fold increase in lateral and axial resolution compared to conventional confocal microscopes by combining QDTI with state‐of‐the‐art commercial laser scanning microscope systems. |
format |
article |
author |
Simon Hennig Dietmar J. Manstein |
author_facet |
Simon Hennig Dietmar J. Manstein |
author_sort |
Simon Hennig |
title |
Improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging |
title_short |
Improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging |
title_full |
Improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging |
title_fullStr |
Improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging |
title_full_unstemmed |
Improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging |
title_sort |
improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging |
publisher |
Wiley |
publishDate |
2021 |
url |
https://doaj.org/article/4a82618becbc4a9ab6586414948d7b61 |
work_keys_str_mv |
AT simonhennig improvementofimageresolutionbycombiningenhancedconfocalmicroscopyandquantumdottriexcitonimaging AT dietmarjmanstein improvementofimageresolutionbycombiningenhancedconfocalmicroscopyandquantumdottriexcitonimaging |
_version_ |
1718405114080788480 |