A 3'-untranslated region (3'UTR) induces organ adhesion by regulating miR-199a* functions.

Mature microRNAs (miRNAs) are single-stranded RNAs of 18-24 nucleotides that repress post-transcriptional gene expression. However, it is unknown whether the functions of mature miRNAs can be regulated. Here we report that expression of versican 3'UTR induces organ adhesion in transgenic mice b...

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Autores principales:	Daniel Y Lee, Tatiana Shatseva, Zina Jeyapalan, William W Du, Zhaoqun Deng, Burton B Yang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4a835a8e4d3a4a4899ef8ba6c2884ca4
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spelling	oai:doaj.org-article:4a835a8e4d3a4a4899ef8ba6c2884ca42021-11-25T06:17:14ZA 3'-untranslated region (3'UTR) induces organ adhesion by regulating miR-199a* functions.1932-620310.1371/journal.pone.0004527https://doaj.org/article/4a835a8e4d3a4a4899ef8ba6c2884ca42009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19223980/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Mature microRNAs (miRNAs) are single-stranded RNAs of 18-24 nucleotides that repress post-transcriptional gene expression. However, it is unknown whether the functions of mature miRNAs can be regulated. Here we report that expression of versican 3'UTR induces organ adhesion in transgenic mice by modulating miR-199a* activities. The study was initiated by the hypothesis that the non-coding 3'UTR plays a role in the regulation of miRNA function. Transgenic mice expressing a construct harboring the 3'UTR of versican exhibits the adhesion of organs. Computational analysis indicated that a large number of microRNAs could bind to this fragment potentially including miR-199a. Expression of versican and fibronectin, two targets of miR-199a, are up-regulated in transgenic mice, suggesting that the 3'UTR binds and modulates miR-199a* activities, freeing mRNAs of versican and fibronectin from being repressed by miR-199a. Confirmation of the binding was performed by PCR using mature miR-199a as a primer and the targeting was performed by luciferase assays. Enhanced adhesion by expression of the 3'UTR was confirmed by in vitro assays. Our results demonstrated that upon arrival in cytoplasm, miRNA activities can be modulated locally by the 3'UTR. Our assay may be developed as sophisticated approaches for studying the mutual regulation of miRNAs and mRNAs in vitro and in vivo. We anticipate that expression of the 3'UTR may be an approach in the development of gene therapy.Daniel Y LeeTatiana ShatsevaZina JeyapalanWilliam W DuZhaoqun DengBurton B YangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 2, p e4527 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daniel Y Lee Tatiana Shatseva Zina Jeyapalan William W Du Zhaoqun Deng Burton B Yang A 3'-untranslated region (3'UTR) induces organ adhesion by regulating miR-199a* functions.
description	Mature microRNAs (miRNAs) are single-stranded RNAs of 18-24 nucleotides that repress post-transcriptional gene expression. However, it is unknown whether the functions of mature miRNAs can be regulated. Here we report that expression of versican 3'UTR induces organ adhesion in transgenic mice by modulating miR-199a* activities. The study was initiated by the hypothesis that the non-coding 3'UTR plays a role in the regulation of miRNA function. Transgenic mice expressing a construct harboring the 3'UTR of versican exhibits the adhesion of organs. Computational analysis indicated that a large number of microRNAs could bind to this fragment potentially including miR-199a. Expression of versican and fibronectin, two targets of miR-199a, are up-regulated in transgenic mice, suggesting that the 3'UTR binds and modulates miR-199a* activities, freeing mRNAs of versican and fibronectin from being repressed by miR-199a. Confirmation of the binding was performed by PCR using mature miR-199a as a primer and the targeting was performed by luciferase assays. Enhanced adhesion by expression of the 3'UTR was confirmed by in vitro assays. Our results demonstrated that upon arrival in cytoplasm, miRNA activities can be modulated locally by the 3'UTR. Our assay may be developed as sophisticated approaches for studying the mutual regulation of miRNAs and mRNAs in vitro and in vivo. We anticipate that expression of the 3'UTR may be an approach in the development of gene therapy.
format	article
author	Daniel Y Lee Tatiana Shatseva Zina Jeyapalan William W Du Zhaoqun Deng Burton B Yang
author_facet	Daniel Y Lee Tatiana Shatseva Zina Jeyapalan William W Du Zhaoqun Deng Burton B Yang
author_sort	Daniel Y Lee
title	A 3'-untranslated region (3'UTR) induces organ adhesion by regulating miR-199a* functions.
title_short	A 3'-untranslated region (3'UTR) induces organ adhesion by regulating miR-199a* functions.
title_full	A 3'-untranslated region (3'UTR) induces organ adhesion by regulating miR-199a* functions.
title_fullStr	A 3'-untranslated region (3'UTR) induces organ adhesion by regulating miR-199a* functions.
title_full_unstemmed	A 3'-untranslated region (3'UTR) induces organ adhesion by regulating miR-199a* functions.
title_sort	3'-untranslated region (3'utr) induces organ adhesion by regulating mir-199a* functions.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/4a835a8e4d3a4a4899ef8ba6c2884ca4
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A 3'-untranslated region (3'UTR) induces organ adhesion by regulating miR-199a* functions.

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