Extensive genome-wide variability of human cytomegalovirus in congenitally infected infants.

Research has shown that RNA virus populations are highly variable, most likely due to low fidelity replication of RNA genomes. It is generally assumed that populations of DNA viruses will be less complex and show reduced variability when compared to RNA viruses. Here, we describe the use of high thr...

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Autores principales:	Nicholas Renzette, Bornali Bhattacharjee, Jeffrey D Jensen, Laura Gibson, Timothy F Kowalik
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/4a88997ffaad481f8da0b957136ece39
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spelling	oai:doaj.org-article:4a88997ffaad481f8da0b957136ece392021-11-18T06:03:22ZExtensive genome-wide variability of human cytomegalovirus in congenitally infected infants.1553-73661553-737410.1371/journal.ppat.1001344https://doaj.org/article/4a88997ffaad481f8da0b957136ece392011-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21625576/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Research has shown that RNA virus populations are highly variable, most likely due to low fidelity replication of RNA genomes. It is generally assumed that populations of DNA viruses will be less complex and show reduced variability when compared to RNA viruses. Here, we describe the use of high throughput sequencing for a genome wide study of viral populations from urine samples of neonates with congenital human cytomegalovirus (HCMV) infections. We show that HCMV intrahost genomic variability, both at the nucleotide and amino acid level, is comparable to many RNA viruses, including HIV. Within intrahost populations, we find evidence of selective sweeps that may have resulted from immune-mediated mechanisms. Similarly, genome wide, population genetic analyses suggest that positive selection has contributed to the divergence of the HCMV species from its most recent ancestor. These data provide evidence that HCMV, a virus with a large dsDNA genome, exists as a complex mixture of genome types in humans and offer insights into the evolution of the virus.Nicholas RenzetteBornali BhattacharjeeJeffrey D JensenLaura GibsonTimothy F KowalikPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 7, Iss 5, p e1001344 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Nicholas Renzette Bornali Bhattacharjee Jeffrey D Jensen Laura Gibson Timothy F Kowalik Extensive genome-wide variability of human cytomegalovirus in congenitally infected infants.
description	Research has shown that RNA virus populations are highly variable, most likely due to low fidelity replication of RNA genomes. It is generally assumed that populations of DNA viruses will be less complex and show reduced variability when compared to RNA viruses. Here, we describe the use of high throughput sequencing for a genome wide study of viral populations from urine samples of neonates with congenital human cytomegalovirus (HCMV) infections. We show that HCMV intrahost genomic variability, both at the nucleotide and amino acid level, is comparable to many RNA viruses, including HIV. Within intrahost populations, we find evidence of selective sweeps that may have resulted from immune-mediated mechanisms. Similarly, genome wide, population genetic analyses suggest that positive selection has contributed to the divergence of the HCMV species from its most recent ancestor. These data provide evidence that HCMV, a virus with a large dsDNA genome, exists as a complex mixture of genome types in humans and offer insights into the evolution of the virus.
format	article
author	Nicholas Renzette Bornali Bhattacharjee Jeffrey D Jensen Laura Gibson Timothy F Kowalik
author_facet	Nicholas Renzette Bornali Bhattacharjee Jeffrey D Jensen Laura Gibson Timothy F Kowalik
author_sort	Nicholas Renzette
title	Extensive genome-wide variability of human cytomegalovirus in congenitally infected infants.
title_short	Extensive genome-wide variability of human cytomegalovirus in congenitally infected infants.
title_full	Extensive genome-wide variability of human cytomegalovirus in congenitally infected infants.
title_fullStr	Extensive genome-wide variability of human cytomegalovirus in congenitally infected infants.
title_full_unstemmed	Extensive genome-wide variability of human cytomegalovirus in congenitally infected infants.
title_sort	extensive genome-wide variability of human cytomegalovirus in congenitally infected infants.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/4a88997ffaad481f8da0b957136ece39
work_keys_str_mv	AT nicholasrenzette extensivegenomewidevariabilityofhumancytomegalovirusincongenitallyinfectedinfants AT bornalibhattacharjee extensivegenomewidevariabilityofhumancytomegalovirusincongenitallyinfectedinfants AT jeffreydjensen extensivegenomewidevariabilityofhumancytomegalovirusincongenitallyinfectedinfants AT lauragibson extensivegenomewidevariabilityofhumancytomegalovirusincongenitallyinfectedinfants AT timothyfkowalik extensivegenomewidevariabilityofhumancytomegalovirusincongenitallyinfectedinfants
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Extensive genome-wide variability of human cytomegalovirus in congenitally infected infants.

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